Reviewed, UniProtKB/Swiss-Prot Q8DBX3 (SYY2_VIBVU)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 2
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 2
      Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	VV1_1683

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Tyrosyl-tRNA synthetase 2 HAMAP MF_02007		PRO_0000236778
Regions
	Domain	334 – 395	62	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02007
	Motif	226 – 230	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8DBX3-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7A9CCBC085158A22	FASTA	396	43,969
	10 20 30 40 50 60 MASIEAALAE IKRGVEELIP EEELIAKLKE GRPLRIKLGA DPTAPDIHLG HTVILNKLRA 70 80 90 100 110 120 FQDLGHDVTF LIGDFTGMVG DPTGKNSTRP PLTREDVLRN AETYKQQVFK ILDPAKTKIQ 130 140 150 160 170 180 FNSEWLSELG AEGMIRLAAN QTVARMLERD DFKKRYAGGQ PIAIHEFMYP LLQGYDSVAM 190 200 210 220 230 240 ETDVELGGTD QKFNLLMGRE LQKANGQKPQ VVLMMPLLVG LDGEKKMSKS ANNYIGVSEA 250 260 270 280 290 300 PSEMFGKIMS ISDDLMWSYY ELLSFRPLEE LAQFKADVAA GKNPRDIKVL LAKEIIARFH 310 320 330 340 350 360 SEADADAAEQ EFVNRFAKNQ IPDEMPEFTF AAGTPMANLL KEAELCSSTS EAMRMVKQGA 370 380 390 AKMDGEKVED AKSRTSPLAP IVFQVGKRKF ARITIA

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016795 Genomic DNA. Translation: AAO10099.1.
RefSeq	NP_760572.1.
3D structure databases
HSSP	HSSP built from PDB template 1H3F based on UniProtKB P83453.
ModBase	Search...
Genome annotation databases
GeneID	1178596.
GenomeReviews	Gene locus VV1_1683 in contig AE016795_GR.
KEGG	vvu:VV1_1683.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8DBX3.
OMA	YVVQVGK.
Enzyme and pathway databases
BioCyc	VVUL216895:VV1_1683-MON.
BRENDA	6.1.1.1. 277169.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_VIBVU

Accession

Primary (citable) accession number: Q8DBX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)