Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi487MagnesiumUniRule annotation1
Metal bindingi493MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:VV1_1708
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003299321 – 708Polyribonucleotide nucleotidyltransferaseAdd BLAST708

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8DBU9.
SMRiQ8DBU9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini554 – 613KHUniRule annotationAdd BLAST60
Domaini623 – 691S1 motifUniRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C62. Bacteria.
COG1185. LUCA.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRYMHNYN.
OrthoDBiPOG091H00M0.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase. 1 hit.
InterProiView protein in InterPro
IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
IPR003029. S1_domain.
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiView protein in Pfam
PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 1 hit.
PF00575. S1. 1 hit.
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiView protein in SMART
SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiView protein in PROSITE
PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8DBU9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFEKPVVKTF QYGNHTVTLE TGVIARQATA AVMVTMDDTA VFVSVVGKKE
60 70 80 90 100
AVPGQDFFPL TVNYQERTYA AGKIPGGFFK REGRPSEGET LTARLIDRPI
110 120 130 140 150
RPLFPEGFNN EVQVIATVVS VNPDVQPDIP TMIGTSAALA ISGIPFNGPI
160 170 180 190 200
GAARVGHIDG QLVLNPSNTE LNASRLDLVV AGTESAVLMV ESEADNLTEE
210 220 230 240 250
EMLSAVVFGH DQQQAVIKAI NEFAAEVATP SWNWVAPEAN TALNEKVADL
260 270 280 290 300
AEAKLVEAYK ITEKMARYDR IHEIAAEVNA VILAEDPEAD AKEIHTIFHD
310 320 330 340 350
LEKTVVRRSI IAGNPRIDGR EKDMVRALDV RTGVLPRTHG SSLFTRGETQ
360 370 380 390 400
ALVTATLGTQ RDAQIIDELT GEKKDYFLLH YNFPPYCVGE TGFVGSPKRR
410 420 430 440 450
EIGHGKLAKR GIAAVMPSID EFPYTVRVVS EITESNGSSS MASVCGTSLA
460 470 480 490 500
LMDAGVPIKA SVAGIAMGLV KEGDDFVVLS DILGDEDHLG DMDFKVAGTS
510 520 530 540 550
TGITALQMDI KIEGITKEIM QIALNQAQGA RKHILSVMDQ AISGARDDIS
560 570 580 590 600
EFAPRIHTMK ISADKIKDVI GKGGAVIRAL TEETGTTIEI EDDGTIKIAA
610 620 630 640 650
TEGAAAKEAI RRIQEITAEV EVGVIYTGKV ARLADFGAFV TILPGKDGLV
660 670 680 690 700
HISQIADKRV EKVSDYLTEG QEVQVKVLEI DRQGRVRLSM KEAVEKPVEA

EAPAAEEE
Length:708
Mass (Da):76,308
Last modified:March 1, 2003 - v1
Checksum:i4A6E7A8A31FF24C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO10123.1.
RefSeqiWP_011079626.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO10123; AAO10123; VV1_1708.
GeneIDi2625505.
KEGGivvu:VV1_1708.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPNP_VIBVU
AccessioniPrimary (citable) accession number: Q8DBU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: March 1, 2003
Last modified: July 5, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families