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Q8DBG3

- GLND_VIBVU

UniProt

Q8DBG3 - GLND_VIBVU

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
    2. amino acid binding Source: InterPro
    3. metal ion binding Source: InterPro
    4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. nitrogen compound metabolic process Source: InterPro
    2. regulation of nitrogen utilization Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BioCyciVVUL216895:GIYM-1642-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
    Short name:
    UTase/URUniRule annotation
    Alternative name(s):
    Bifunctional [protein-PII] modification enzymeUniRule annotation
    Bifunctional nitrogen sensor proteinUniRule annotation
    Including the following 2 domains:
    [Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
    Short name:
    PII uridylyltransferaseUniRule annotation
    Short name:
    UTaseUniRule annotation
    [Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
    Short name:
    URUniRule annotation
    Gene namesi
    Name:glnDUniRule annotation
    Ordered Locus Names:VV1_1857
    OrganismiVibrio vulnificus (strain CMCP6)
    Taxonomic identifieri216895 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000002275: Chromosome 1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192774Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi216895.VV1_1857.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8DBG3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini452 – 584133HDUniRule annotationAdd
    BLAST
    Domaini693 – 77381ACT 1UniRule annotationAdd
    BLAST
    Domaini800 – 87374ACT 2UniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 332332UridylyltransferaseAdd
    BLAST
    Regioni333 – 692360Uridylyl-removingAdd
    BLAST

    Domaini

    Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

    Sequence similaritiesi

    Belongs to the GlnD family.UniRule annotation
    Contains 2 ACT domains.UniRule annotation
    Contains 1 HD domain.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000261778.
    KOiK00990.
    OMAiHHLLMSV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    HAMAPiMF_00277. PII_uridylyl_transf.
    InterProiIPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
    PROSITEiPS51671. ACT. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8DBG3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFQSPLTFN DEQLTVAQLK SQLDLFANAQ KQAFLNHHPV TDLVLSRAEY    50
    MDLLLTRLWR YYGFSEIHNI SLVAVGGYGR GELHPLSDID ILVLSKHKLP 100
    GELETKLSEF ITLLWDLRLE VGHAVRTVEE CAAIGREDLT VATNLQEARL 150
    LCGSENTFQD LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP 200
    DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD 250
    FLWRVRFALH IELKRYDNRL TFAHQIQVAE HLGFKGEGNR GIEMMMKEFY 300
    RTLRRVAELN KMLLKLFDQA IINGGETEPA VIINEDFQRR GRLIEARKPA 350
    LFQARPETIL DMFLHIANDS TIDSVSPPTL RQLRTARRRL NKFLHTIPEA 400
    REKFMELVRH PNALHRAFSL MHKLGVLAAY LPQWSQIVGQ MQFDLFHVYT 450
    VDEHSVRLLN HINTFSYAKN HDKHPICCEV YPRLQKKELL LLAAIFHDIG 500
    KGRGGDHSEI GEKEAYDFCI EHGLSKPEAK LVSWLVRHHL LMSVTAQRRD 550
    IYDPEVITEF AKQVRDEERL EYLVCLTVAD ICATNPELWN SWKRTLLAEL 600
    FYSTQRALRR GLENPVDVRE RIRHNQQLAS ALLRKEGFTA REIEVLWQRF 650
    KADYFLRHTH KQIAWHCEHI LRMDNPEQPL VLMSKKATRG GTEVFVYTKD 700
    QHALFATVVA ELDRRNFNVH DAQIMSSKDG YVLDTFMVLD QHGQAIDVDN 750
    HKAVIKHLMH VLADGRPTKV KTRRTPYKLQ HFKVKTKVDF LPTKSKKRTL 800
    MELVALDTPG LLAITGATFA DMGFNLHGAK ITTIGERAED LFILTSENGG 850
    RLSEEQELQL REKLIHNIAE LAP 873
    Length:873
    Mass (Da):101,030
    Last modified:March 1, 2003 - v1
    Checksum:iEF8625318CD2C16B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016795 Genomic DNA. Translation: AAO10260.1.
    RefSeqiNP_760733.1. NC_004459.3.

    Genome annotation databases

    EnsemblBacteriaiAAO10260; AAO10260; VV1_1857.
    GeneIDi1178759.
    KEGGivvu:VV1_1857.
    PATRICi20160496. VBIVibVul94426_1729.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016795 Genomic DNA. Translation: AAO10260.1 .
    RefSeqi NP_760733.1. NC_004459.3.

    3D structure databases

    ProteinModelPortali Q8DBG3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216895.VV1_1857.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO10260 ; AAO10260 ; VV1_1857 .
    GeneIDi 1178759.
    KEGGi vvu:VV1_1857.
    PATRICi 20160496. VBIVibVul94426_1729.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000261778.
    KOi K00990.
    OMAi HHLLMSV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BioCyci VVUL216895:GIYM-1642-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    HAMAPi MF_00277. PII_uridylyl_transf.
    InterProi IPR002912. ACT_dom.
    IPR010043. GlnD_Uridyltrans.
    IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF01842. ACT. 1 hit.
    PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
    PROSITEi PS51671. ACT. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Vibrio vulnificus CMCP6."
      Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CMCP6.

    Entry informationi

    Entry nameiGLND_VIBVU
    AccessioniPrimary (citable) accession number: Q8DBG3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 23, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3