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Q8DBG3

- GLND_VIBVU

UniProt

Q8DBG3 - GLND_VIBVU

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, VV1_1857
Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-1642-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:VV1_1857
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002275: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_0000192774Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi216895.VV1_1857.

Structurei

3D structure databases

ProteinModelPortaliQ8DBG3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini452 – 584133HD
Add
BLAST
Domaini693 – 77381ACT 1
Add
BLAST
Domaini800 – 87374ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseUniRule annotation
Add
BLAST
Regioni333 – 692360Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8DBG3-1 [UniParc]FASTAAdd to Basket

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MAFQSPLTFN DEQLTVAQLK SQLDLFANAQ KQAFLNHHPV TDLVLSRAEY    50
MDLLLTRLWR YYGFSEIHNI SLVAVGGYGR GELHPLSDID ILVLSKHKLP 100
GELETKLSEF ITLLWDLRLE VGHAVRTVEE CAAIGREDLT VATNLQEARL 150
LCGSENTFQD LKKVVLSDSF WPSETFYRAK IQEQRERHAR YHDTTYNLEP 200
DIKSTPGGLR DIHTLSWVAR RHFGATSLLE MSRYGFLTDA EYRELVECQD 250
FLWRVRFALH IELKRYDNRL TFAHQIQVAE HLGFKGEGNR GIEMMMKEFY 300
RTLRRVAELN KMLLKLFDQA IINGGETEPA VIINEDFQRR GRLIEARKPA 350
LFQARPETIL DMFLHIANDS TIDSVSPPTL RQLRTARRRL NKFLHTIPEA 400
REKFMELVRH PNALHRAFSL MHKLGVLAAY LPQWSQIVGQ MQFDLFHVYT 450
VDEHSVRLLN HINTFSYAKN HDKHPICCEV YPRLQKKELL LLAAIFHDIG 500
KGRGGDHSEI GEKEAYDFCI EHGLSKPEAK LVSWLVRHHL LMSVTAQRRD 550
IYDPEVITEF AKQVRDEERL EYLVCLTVAD ICATNPELWN SWKRTLLAEL 600
FYSTQRALRR GLENPVDVRE RIRHNQQLAS ALLRKEGFTA REIEVLWQRF 650
KADYFLRHTH KQIAWHCEHI LRMDNPEQPL VLMSKKATRG GTEVFVYTKD 700
QHALFATVVA ELDRRNFNVH DAQIMSSKDG YVLDTFMVLD QHGQAIDVDN 750
HKAVIKHLMH VLADGRPTKV KTRRTPYKLQ HFKVKTKVDF LPTKSKKRTL 800
MELVALDTPG LLAITGATFA DMGFNLHGAK ITTIGERAED LFILTSENGG 850
RLSEEQELQL REKLIHNIAE LAP 873
Length:873
Mass (Da):101,030
Last modified:March 1, 2003 - v1
Checksum:iEF8625318CD2C16B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016795 Genomic DNA. Translation: AAO10260.1.
RefSeqiNP_760733.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO10260; AAO10260; VV1_1857.
GeneIDi1178759.
KEGGivvu:VV1_1857.
PATRICi20160496. VBIVibVul94426_1729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016795 Genomic DNA. Translation: AAO10260.1 .
RefSeqi NP_760733.1. NC_004459.3.

3D structure databases

ProteinModelPortali Q8DBG3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216895.VV1_1857.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO10260 ; AAO10260 ; VV1_1857 .
GeneIDi 1178759.
KEGGi vvu:VV1_1857.
PATRICi 20160496. VBIVibVul94426_1729.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci VVUL216895:GIYM-1642-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiGLND_VIBVU
AccessioniPrimary (citable) accession number: Q8DBG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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