Q8D9M7 (NAGK_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-D-glucosamine kinase
EC=2.7.1.59

Alternative name(s):
GlcNAc kinase

Gene names

Name:	nagK
Ordered Locus Names:	VV1_2570

Organism

Vibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]

Taxonomic identifier

216895 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio › Vibrio vulnificus

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the phosphorylation of N-acetyl-D-glucosamine (GlcNAc) derived from cell-wall degradation, yielding GlcNAc-6-P By similarity. HAMAP-Rule MF_01271
Catalytic activity	ATP + N-acetyl-D-glucosamine = ADP + N-acetyl-D-glucosamine 6-phosphate. HAMAP-Rule MF_01271
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_01271
Sequence similarities	Belongs to the ROK (NagC/XylR) family. NagK subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological_process	N-acetylglucosamine metabolic process Inferred from electronic annotation. Source: HAMAP peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP N-acetylglucosamine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 303

303

N-acetyl-D-glucosamine kinase HAMAP-Rule MF_01271

PRO_0000270122

Regions

Nucleotide binding

4 – 11

8

ATP Potential

Nucleotide binding

133 – 140

8

ATP Potential

Sites

Metal binding

157

1

Zinc By similarity

Metal binding

177

1

Zinc By similarity

Metal binding

179

1

Zinc By similarity

Metal binding

184

1

Zinc By similarity

Secondary structure

1

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303

Helix Strand Turn

Details...

Beta strand

2 – 7

6

Beta strand

9 – 17

9

Beta strand

23 – 30

8

Helix

36 – 54

19

Beta strand

59 – 68

10

Turn

70 – 72

3

Beta strand

75 – 80

6

Helix

81 – 83

3

Helix

88 – 96

9

Beta strand

101 – 104

4

Helix

105 – 114

10

Turn

117 – 121

5

Beta strand

123 – 140

18

Helix

155 – 157

3

Helix

162 – 167

6

Beta strand

182 – 184

3

Helix

185 – 187

3

Helix

191 – 202

12

Helix

208 – 217

10

Helix

220 – 244

25

Beta strand

247 – 253

7

Helix

254 – 257

4

Helix

260 – 268

9

Helix

269 – 272

4

Beta strand

281 – 284

4

Helix

288 – 290

3

Helix

291 – 298

8

Sequences

	Sequence		Length	Mass (Da)
	Q8D9M7 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: F1804F008B123B61	FASTA	303	32,570
	10 20 30 40 50 60 MYYGFDVGGT KIEFGAFNEK LERVATERVP TPTDDYPLLL ETIAGLVAKY DQEFACEGKI 70 80 90 100 110 120 GLGLPGMEDA DDATVLTVNV PAAKGKPLRA DLEAKIGRSV KIENDANCFA LSEAWDEELQ 130 140 150 160 170 180 DAPSVMGLIL GTGFGGGLIY EGKVFSGRNN VAGELGHMRL PLDAWFHLGD NAPLLGCGCG 190 200 210 220 230 240 KKGCLDSYLS GRGFELLYAH YYGEEKKAID IIKANAAGDE KAAEHVERFM ELLAICFGNI 250 260 270 280 290 300 FTANDPHVVA LGGGLSNFEL IYEEMPKRVP KYLLSVAKCP KIIKAKHGDS GGVRGAAFLN IKG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.
[2]	"Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug targeting and discovery." Kim H.U., Kim S.Y., Jeong H., Kim T.Y., Kim J.J., Choy H.E., Yi K.Y., Rhee J.H., Lee S.Y. Mol. Syst. Biol. 7:460-460(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 297.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE016795 Genomic DNA. Translation: AAO10921.2.

RefSeq

NP_761394.2. NC_004459.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4DB3	X-ray	1.95	A	1-303	[»]

ProteinModelPortal

Q8D9M7.

SMR

Q8D9M7. Positions 1-302.

ModBase

Search...

Protein-protein interaction databases

STRING

216895.VV1_2570.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAO10921; AAO10921; VV1_2570.

GeneID

1179422.

KEGG

vvu:VV1_2570.

PATRIC

20162134. VBIVibVul94426_2545.

Organism-specific databases

CMR

Search...

Phylogenomic databases

eggNOG

COG1940.

HOGENOM

HOG000150087.

KO

K00884.

ProtClustDB

PRK13310.

Enzyme and pathway databases

UniPathway

UPA00544.

Family and domain databases

HAMAP

MF_01271. GlcNAc_kinase.

InterPro

IPR023505. N-acetyl-D-glucosamine_kinase.
IPR000600. ROK.
[Graphical view]

Pfam

PF00480. ROK. 1 hit.
[Graphical view]

PROSITE

PS01125. ROK. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

NAGK_VIBVU

Accession

Primary (citable) accession number: Q8D9M7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program