ID   MAO1_VIBVU              Reviewed;         562 AA.
AC   Q8D911;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   16-JUN-2009, entry version 44.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=VV1_2801;
OS   Vibrio vulnificus.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H.,
RA   Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; AE016795; AAO11140.1; -; Genomic_DNA.
DR   RefSeq; NP_761613.1; -.
DR   HSSP; P40927; 1GQ2.
DR   GeneID; 1179643; -.
DR   GenomeReviews; AE016795_GR; VV1_2801.
DR   KEGG; vvu:VV1_2801; -.
DR   HOGENOM; Q8D911; -.
DR   OMA; Q8D911; IQDNNET.
DR   BioCyc; VVUL216895:VV1_2801-MON; -.
DR   BRENDA; 1.1.1.38; 277169.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    562       NAD-dependent malic enzyme.
FT                                /FTId=PRO_0000160237.
FT   ACT_SITE    101    101       Proton donor (By similarity).
FT   ACT_SITE    172    172       Proton acceptor (By similarity).
FT   METAL       243    243       Divalent metal cation (By similarity).
FT   METAL       244    244       Divalent metal cation (By similarity).
FT   METAL       267    267       Divalent metal cation (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     267    267       NAD (By similarity).
FT   BINDING     415    415       NAD (By similarity).
FT   SITE        267    267       Important for activity (By similarity).
SQ   SEQUENCE   562 AA;  62088 MW;  F05F1BC579ABBAB1 CRC64;
     MNNDKRPLYI PFAGPALLST PLLNKGSAFS AEERISFNLE GLLPETTETI QEQVERAYMQ
     YKAFESDMDK HIYLRNIQDT NETLFYRLVQ NHITEMMPII YTPTVGAACE NFSNIYRRGR
     GLFVSYANRD RIDDILNNAS NHNVKVIVVT DGERILGLGD QGIGGMGIPI GKLSLYTACG
     GISPAYTLPI VLDVGTNNPQ RLADPMYMGW RHPRITGADY DAFVEEFIQA VQRRWPDALI
     QFEDFAQKNA MPLLERYKNR ICCFNDDIQG TAAVTVGSLM AACQAAGSKL SEQRITFLGA
     GSAGCGIAEA IIAQMVSEGI SDKKARSQVF MVDRWGLLQE GMPNLLDFQQ RLVQKHSVTA
     KWETEANGFS LLDVVKNAKP TVLIGVSGAP GLFTQEVIQE MHKHCPRPIV FPLSNPTSRV
     EAVPADIIRW TNGDALVATG SPFDPVIHEG KTYPIVQCNN SYIFPGIGLG VLAVNAKRVT
     DEMLMESSRA LATCSPLAIN GKGALLPPLE EIHTVSKRIA YAVAKKAIEQ GVALEIADDA
     LQVAIEQHFW QPVYRRYKRT AF
//