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Protein

UDP-2,3-diacylglucosamine hydrolase

Gene

lpxH

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of the pyrophosphate bond of UDP-2,3-diacylglucosamine to yield 2,3-diacylglucosamine 1-phosphate (lipid X) and UMP.UniRule annotation

Catalytic activityi

UDP-2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-alpha-D-glucosamine + H2O = 2-N,3-O-bis((3R)-3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UMP.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. UDP-2,3-diacylglucosamine hydrolase (lpxH)
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-2,3-diacylglucosamine hydrolaseUniRule annotation (EC:3.6.1.54UniRule annotation)
Alternative name(s):
UDP-2,3-diacylglucosamine diphosphataseUniRule annotation
Gene namesi
Name:lpxHUniRule annotation
Ordered Locus Names:VV1_2910
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002141271 – 243UDP-2,3-diacylglucosamine hydrolaseAdd BLAST243

Interactioni

Protein-protein interaction databases

STRINGi216895.VV1_2910.

Structurei

3D structure databases

ProteinModelPortaliQ8D8Q9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LpxH family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F10. Bacteria.
COG2908. LUCA.
HOGENOMiHOG000261930.
KOiK03269.
OMAiFMHGNRD.
OrthoDBiPOG091H064W.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00575. LpxH. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8D8Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTLFISDLH LTPSRTDITE CFVQFMRNEA VNAEALYVLG DLFEFWIGDE
60 70 80 90 100
DCTPFAERIR NEFKALTTSG VPVYFIQGNR DFLLGQRFCR ETGITLLDDV
110 120 130 140 150
CTIDLYGEKV VILHGDTLCI DDLKYQEFRK TVHQRWLQWI FKRIPWFIKK
160 170 180 190 200
RIVAKVQSGV RDDKQHKSLE IMDVNQQEVA QVMSQFCVKL MIHGHTHRPN
210 220 230 240
IHHFEHDNLP LTRIVLGDWY SQGSVLKVTA DGYSLEQRPF FTE
Length:243
Mass (Da):28,461
Last modified:May 24, 2004 - v2
Checksum:i7DE3AE02E760234F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO11243.2.
RefSeqiWP_011080730.1. NC_004459.3.

Genome annotation databases

EnsemblBacteriaiAAO11243; AAO11243; VV1_2910.
KEGGivvu:VV1_2910.
PATRICi20162797. VBIVibVul94426_2873.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016795 Genomic DNA. Translation: AAO11243.2.
RefSeqiWP_011080730.1. NC_004459.3.

3D structure databases

ProteinModelPortaliQ8D8Q9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV1_2910.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO11243; AAO11243; VV1_2910.
KEGGivvu:VV1_2910.
PATRICi20162797. VBIVibVul94426_2873.

Phylogenomic databases

eggNOGiENOG4105F10. Bacteria.
COG2908. LUCA.
HOGENOMiHOG000261930.
KOiK03269.
OMAiFMHGNRD.
OrthoDBiPOG091H064W.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00480.

Family and domain databases

Gene3Di3.60.21.10. 2 hits.
HAMAPiMF_00575. LpxH. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR010138. UDP-diacylglucosamine_Hdrlase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
TIGRFAMsiTIGR01854. lipid_A_lpxH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiLPXH_VIBVU
AccessioniPrimary (citable) accession number: Q8D8Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 24, 2004
Last modified: November 30, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.