Q8D8N2 (BIOD_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	VV1_2940

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 227	227	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187995
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
	Nucleotide binding	116 – 119	4	ATP By similarity
	Nucleotide binding	176 – 177	2	ATP By similarity
	Nucleotide binding	205 – 207	3	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	55	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D8N2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 629A4A84ED74BFFD	FASTA	227	24,636
	10 20 30 40 50 60 MMKAFFIAGT DTDVGKTVAS KAILHALAAK DLRTIGYKPV AAGSDKTPEG WRNSDALHLQ 70 80 90 100 110 120 KAATVAVDYE DINPYALELP ASPHIAAKHE KVEIDYAVLS RKLAQHKENA DVVLVEGAGG 130 140 150 160 170 180 WRVPVSDTDS LSTWVKQEDL PVVLVVGIKL GCLSHALLTA EVIKADGLNL VGWVANRVNP 190 200 210 220 GTEHYAEIID MLEERLEVPK LGEIPYIPSA KRKDLGKYIN ADVLLEL

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016795 Genomic DNA. Translation: AAO11271.1.
RefSeq	NP_761744.1. NC_004459.3.
3D structure databases
ProteinModelPortal	Q8D8N2.
SMR	Q8D8N2. Positions 3-224.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1179774.
GenomeReviews	Gene locus VV1_2940 in contig AE016795_GR.
KEGG	vvu:VV1_2940.
PATRIC	20162855. VBIVibVul94426_2902.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	ALAAYWQ.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	VVUL216895:VV1_2940-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_VIBVU

Accession

Primary (citable) accession number: Q8D8N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program