Q8D8E2 (Q8D8E2_VIBVU) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A HAMAP MF_01897
EC=5.99.1.3 HAMAP MF_01897

Gene names

Name:	gyrA HAMAP MF_01897 EMBL AAO11362.2
Ordered Locus Names:	VV1_3038

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	903 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings By similarity. SAAS SAAS006691 HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. SAAS SAAS006691 HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer By similarity. HAMAP MF_01897
Subcellular location	Cytoplasm By similarity SAAS SAAS006691 HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family. HAMAP MF_01897

Ontologies

Keywords
Cellular component	Cytoplasm SAAS SAAS006691 HAMAP MF_01897
Ligand	ATP-binding SAAS SAAS006691 HAMAP MF_01897 DNA-binding SAAS SAAS006691 HAMAP MF_01897 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS006691 HAMAP MF_01897
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q8D8E2 [UniParc]. Last modified April 5, 2011. Version 2. Checksum: CF02E0D859CCC9C9	FASTA	903	99,820
	10 20 30 40 50 60 MSDLAKEITP VNIEDELRGS YLDYAMSVIV GRALPDVRDG LKPVHRRVLY AMNVLGNDWN 70 80 90 100 110 120 KPYKKSARVV GDVIGKYHPH GDSAVYDTIV RMAQPFSLRY MLVDGQGNFG SIDGDSAAAM 130 140 150 160 170 180 RYTEVRMAKI AHELLADLDK ETVDYVPNYD GTEQIPAVLP TKIPNLLVNG SSGIAVGMAT 190 200 210 220 230 240 NIPPHNLTEV IDGCLAYINN EAITIDELMD YIPGPDFPTA ALISGRKGII DAYKTGRGKI 250 260 270 280 290 300 YMRSKAEIEV EKNGKETIIV TEIPYQVNKA RLIEKIAELV KDKKVEGISA LRDESDKDGM 310 320 330 340 350 360 RIVIECKRDA VGEVVLNNLY AQTQLQTTFG INMVALDNGQ PKLFNLKEML KCFVDHRREV 370 380 390 400 410 420 VTRRTIFELK KARDRAHILE ALALALANID EIIELIRNAA TPAEAKAGLV ARGWDLGNVA 430 440 450 460 470 480 AMLESAGTDA ARPDWLEPQY GIRDGQYFLT ETQAQAILDL RLHKLTGLEH EKILDEYKAL 490 500 510 520 530 540 LDEIKELMHI LASTERLMEV IREELEMVRD AFGDVRRTEI TAASHDIDLE ELIAREDVVV 550 560 570 580 590 600 TLSHEGYVKY QILSDYEAQR RGGKGKSATK MKDEDYIERL LVANTHDNIL LFSTRGKTYR 610 620 630 640 650 660 LKVYQLPLAS RTARGKPIVN LLPLEESERI TAILPVTEFS ADKFIFMATG DGTVKKTSLD 670 680 690 700 710 720 QFSNVRANGL IAVNLRDEDS LIGVDITDGE SEIMLFSKFG KVVRFKEAEE SPVLDENGNP 730 740 750 760 770 780 ALDENGQPEI KFKGVRPMGR TAAGVRGMKL ADGDQVVSLI VPKNDGDVLT VTENGYGKRT 790 800 810 820 830 840 VLSEYPTKGR GTQGVVSIKV SERNGSVVGA VQVEDGDEFM MITDAGTLVR TRVAEVSQVG 850 860 870 880 890 900 RNTQGVTLIR TAEDESVVGL QRIDEVEETE LPEGEEADAT EATEQAPQAQ DPQADNDDAA DQE

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016795 Genomic DNA. Translation: AAO11362.2.
RefSeq	NP_761835.2. NC_004459.3.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1179865.
GenomeReviews	Gene locus VV1_3038 in contig AE016795_GR.
KEGG	vvu:VV1_3038.
PATRIC	20163049. VBIVibVul94426_2999.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BioCyc	VVUL216895:VV1_3038-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q8D8E2_VIBVU

Accession

Primary (citable) accession number: Q8D8E2

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	April 5, 2011
Last modified:	January 25, 2012
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)