ID   FTHS_VIBVU              Reviewed;         582 AA.
AC   Q8D7D8; Q8D7D9;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-DEC-2005, sequence version 2.
DT   24-JAN-2024, entry version 107.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=VV2_0216; ORFNames=VV2_0217;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=CMCP6;
RX   PubMed=21245845; DOI=10.1038/msb.2010.115;
RA   Kim H.U., Kim S.Y., Jeong H., Kim T.Y., Kim J.J., Choy H.E., Yi K.Y.,
RA   Rhee J.H., Lee S.Y.;
RT   "Integrative genome-scale metabolic analysis of Vibrio vulnificus for drug
RT   targeting and discovery.";
RL   Mol. Syst. Biol. 7:460-460(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; AE016796; AAO07187.2; -; Genomic_DNA.
DR   RefSeq; WP_011081194.1; NC_004460.2.
DR   AlphaFoldDB; Q8D7D8; -.
DR   SMR; Q8D7D8; -.
DR   KEGG; vvu:VV2_0216; -.
DR   HOGENOM; CLU_003601_3_3_6; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000002275; Chromosome 2.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..582
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_0000199410"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   582 AA;  62201 MW;  25EDDDE568C8D299 CRC64;
     MLSDIDICRT TPLKPIEQIA AKAGLLADEF ETHGLYKAKV SARCVKRLAE QQDGKLVLVT
     AITPTPLGEG KTVTTIGLAQ GLQSLNQSVM ACIRQPSMGP VFGVKGGAAG GGYSQVAPMD
     ELNLHLTGDI HAVTAAHNLA AAAIDARIYH EQRAGYQAFE ARTGLPALRI DPDRVVWKRV
     MDHNDRALRK VRVGINDEGK TINGFEREEG FDISAASELM AILALSKNLQ DMRERIGKVV
     LAYNLDSEPV SAEQLQVAGA MTVTLKEAIK PTLMQTLEGV PTLIHAGPFA NIAHGNSSII
     ADEIALKLSS YVVTEAGFGS DMGLEKACNI KSSTSHKAPD CVVIVATLRG LKANSGLYDL
     KPGMPLPDAL FQPDRAALES GFSNLKWHID NAQKYGLPVV VAINQFPQDS AEELGQLKLW
     IQQLPLNVRV AISEGFAKGG KGMEAVAREV IEACDAPANF RPLYRAEQTL EEKIITVCTK
     GYGCGEVQFS DTAKQQLALY QTLGFGELAV CMAKTPLSIS SDSSLKGAPS GFTIMVREVR
     LCAGAGFVYA LTGNVMTMPG LPDIPAFMAL DIDENGDIVG LS
//