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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi51Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi55Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi83Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei85Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei152Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei177Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei181Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei378Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei484Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei533Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei560Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei794Substrate; via amide nitrogenUniRule annotation1
Binding sitei802Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei819Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciVVUL216895:G1GJ4-3915-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:VV2_0721
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Tat-type signalUniRule annotationAdd BLAST30
ChainiPRO_000004601231 – 829Periplasmic nitrate reductaseUniRule annotationAdd BLAST799

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8D623
SMRiQ8D623
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 974Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni214 – 221Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni245 – 249Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni264 – 266Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni510 – 511Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni718 – 727Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
KOiK02567
OMAiANSMGTR
OrthoDBiPOG091H060P

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
IPR006311 TAT_signal
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS51318 TAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8D623-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMTRRAFVK ANAAASAAAV AGITLPASAA NLIASSDQTK ITWDKAPCRF
60 70 80 90 100
CGTGCSVLVG TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGQDRLT
110 120 130 140 150
QPLLRMKDGK YDKEGDFTPV SWDVAFDTMA EKWKASLAKK GPTSIGMFGS
160 170 180 190 200
GQWTVMEGYA AAKMMKAGFR SNNIDPNARH CMASAVVGFM RTFGIDEPMG
210 220 230 240 250
CYDDFEHADS FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK VNVLSTYYHR
260 270 280 290 300
SFELADSGYI FKPQSDLAIA NFIANYIIQN DAVNWDFVNK HTNFKQATTD
310 320 330 340 350
IGYGLRDDDP LQKQAKNPNS GNMTSISFEE YKKSVAPYTV EKASEMSGVA
360 370 380 390 400
QDKLIELAKQ YADPNTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS
410 420 430 440 450
TPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADMVVANPKH RAIAEKIWKL
460 470 480 490 500
PEGTIPPKPG FHAVLQDRML HDGVLNCYWV QCNNNMQAGP NINGERLPGY
510 520 530 540 550
RNPENFIVVS DPYPTATAQA ADLILPTAMW IEKEGAYGNA ERRTQAWYQQ
560 570 580 590 600
VGTVGEAKSD LWQVMEFSKR FKMEEVWPEE LLAKAPQYRG KTMYDMLFAN
610 620 630 640 650
GQVDKFPLSE ARQLNDDSHH FGFYVQKGLF EEYAEFGRGH GHDLAPYDVY
660 670 680 690 700
HTVRGLRWPV VDGKETLWRY KEGSDPYAKK GSGWDFYGKP DGKALIISAP
710 720 730 740 750
YEAPPESPDA EYDMWLCTGR VLEHWHTGTM TRRVPELYKA VPDAVCYIHP
760 770 780 790 800
EDAKARGLRR GDEVLISNKR GEVRVRVETR GRNRPPQGLV FVPFFDARIL
810 820
INKLILDATD PLSKQTDFKK CPVKITKVA
Length:829
Mass (Da):92,816
Last modified:March 1, 2003 - v1
Checksum:i9AF0DD03181FA6E3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016796 Genomic DNA Translation: AAO07656.1
RefSeqiWP_011081654.1, NC_004460.2

Genome annotation databases

EnsemblBacteriaiAAO07656; AAO07656; VV2_0721
KEGGivvu:VV2_0721

Similar proteinsi

Entry informationi

Entry nameiNAPA_VIBVU
AccessioniPrimary (citable) accession number: Q8D623
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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