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Q8D623

- NAPA_VIBVU

UniProt

Q8D623 - NAPA_VIBVU

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Protein
Periplasmic nitrate reductase
Gene
napA, VV2_0721
Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster By similarity.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S) By similarity
Metal bindingi51 – 511Iron-sulfur (4Fe-4S) By similarity
Metal bindingi55 – 551Iron-sulfur (4Fe-4S) By similarity
Metal bindingi83 – 831Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-3557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase (EC:1.7.99.4)
Gene namesi
Name:napA
Ordered Locus Names:VV2_0721
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002275: Chromosome 2

Subcellular locationi

Periplasm By similarity UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signal Reviewed prediction
Add
BLAST
Chaini31 – 829799Periplasmic nitrate reductaseUniRule annotation
PRO_0000046012Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.UniRule annotation

Interactioni

Subunit structurei

Interacts with NapB By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV2_0721.

Structurei

3D structure databases

ProteinModelPortaliQ8D623.
SMRiQ8D623. Positions 41-827.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiEYRGKTM.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8D623-1 [UniParc]FASTAAdd to Basket

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MKMTRRAFVK ANAAASAAAV AGITLPASAA NLIASSDQTK ITWDKAPCRF    50
CGTGCSVLVG TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGQDRLT 100
QPLLRMKDGK YDKEGDFTPV SWDVAFDTMA EKWKASLAKK GPTSIGMFGS 150
GQWTVMEGYA AAKMMKAGFR SNNIDPNARH CMASAVVGFM RTFGIDEPMG 200
CYDDFEHADS FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK VNVLSTYYHR 250
SFELADSGYI FKPQSDLAIA NFIANYIIQN DAVNWDFVNK HTNFKQATTD 300
IGYGLRDDDP LQKQAKNPNS GNMTSISFEE YKKSVAPYTV EKASEMSGVA 350
QDKLIELAKQ YADPNTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS 400
TPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADMVVANPKH RAIAEKIWKL 450
PEGTIPPKPG FHAVLQDRML HDGVLNCYWV QCNNNMQAGP NINGERLPGY 500
RNPENFIVVS DPYPTATAQA ADLILPTAMW IEKEGAYGNA ERRTQAWYQQ 550
VGTVGEAKSD LWQVMEFSKR FKMEEVWPEE LLAKAPQYRG KTMYDMLFAN 600
GQVDKFPLSE ARQLNDDSHH FGFYVQKGLF EEYAEFGRGH GHDLAPYDVY 650
HTVRGLRWPV VDGKETLWRY KEGSDPYAKK GSGWDFYGKP DGKALIISAP 700
YEAPPESPDA EYDMWLCTGR VLEHWHTGTM TRRVPELYKA VPDAVCYIHP 750
EDAKARGLRR GDEVLISNKR GEVRVRVETR GRNRPPQGLV FVPFFDARIL 800
INKLILDATD PLSKQTDFKK CPVKITKVA 829
Length:829
Mass (Da):92,816
Last modified:March 1, 2003 - v1
Checksum:i9AF0DD03181FA6E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016796 Genomic DNA. Translation: AAO07656.1.
RefSeqiNP_762666.1. NC_004460.2.
WP_011081654.1. NC_004460.2.

Genome annotation databases

EnsemblBacteriaiAAO07656; AAO07656; VV2_0721.
GeneIDi1180709.
KEGGivvu:VV2_0721.
PATRICi20164842. VBIVibVul94426_3881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016796 Genomic DNA. Translation: AAO07656.1 .
RefSeqi NP_762666.1. NC_004460.2.
WP_011081654.1. NC_004460.2.

3D structure databases

ProteinModelPortali Q8D623.
SMRi Q8D623. Positions 41-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216895.VV2_0721.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO07656 ; AAO07656 ; VV2_0721 .
GeneIDi 1180709.
KEGGi vvu:VV2_0721.
PATRICi 20164842. VBIVibVul94426_3881.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi EYRGKTM.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci VVUL216895:GIYM-3557-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiNAPA_VIBVU
AccessioniPrimary (citable) accession number: Q8D623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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