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Q8D623

- NAPA_VIBVU

UniProt

Q8D623 - NAPA_VIBVU

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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster.UniRule annotation
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi51 – 511Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi55 – 551Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi83 – 831Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. electron carrier activity Source: UniProtKB-HAMAP
  3. iron ion binding Source: UniProtKB-HAMAP
  4. molybdenum ion binding Source: InterPro
  5. nitrate reductase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-HAMAP
  2. nitrate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-3557-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:VV2_0721
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002275: Chromosome 2

Subcellular locationi

Periplasm UniRule annotation

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3030Tat-type signalUniRule annotationAdd
BLAST
Chaini31 – 829799Periplasmic nitrate reductasePRO_0000046012Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV2_0721.

Structurei

3D structure databases

ProteinModelPortaliQ8D623.
SMRiQ8D623. Positions 41-827.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini41 – 97574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
KOiK02567.
OMAiEYRGKTM.
OrthoDBiEOG6CVV7G.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8D623 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMTRRAFVK ANAAASAAAV AGITLPASAA NLIASSDQTK ITWDKAPCRF
60 70 80 90 100
CGTGCSVLVG TQNGKVVATQ GDPEAPVNKG LNCIKGYFLS KIMYGQDRLT
110 120 130 140 150
QPLLRMKDGK YDKEGDFTPV SWDVAFDTMA EKWKASLAKK GPTSIGMFGS
160 170 180 190 200
GQWTVMEGYA AAKMMKAGFR SNNIDPNARH CMASAVVGFM RTFGIDEPMG
210 220 230 240 250
CYDDFEHADS FVLWGSNMAE MHPVLWTRIT DRRLSHPHVK VNVLSTYYHR
260 270 280 290 300
SFELADSGYI FKPQSDLAIA NFIANYIIQN DAVNWDFVNK HTNFKQATTD
310 320 330 340 350
IGYGLRDDDP LQKQAKNPNS GNMTSISFEE YKKSVAPYTV EKASEMSGVA
360 370 380 390 400
QDKLIELAKQ YADPNTKVMS LWTMGMNQHT RGVWMNSLVY NIHLLTGKIS
410 420 430 440 450
TPGNSPFSLT GQPSACGTAR EVGTFAHRLP ADMVVANPKH RAIAEKIWKL
460 470 480 490 500
PEGTIPPKPG FHAVLQDRML HDGVLNCYWV QCNNNMQAGP NINGERLPGY
510 520 530 540 550
RNPENFIVVS DPYPTATAQA ADLILPTAMW IEKEGAYGNA ERRTQAWYQQ
560 570 580 590 600
VGTVGEAKSD LWQVMEFSKR FKMEEVWPEE LLAKAPQYRG KTMYDMLFAN
610 620 630 640 650
GQVDKFPLSE ARQLNDDSHH FGFYVQKGLF EEYAEFGRGH GHDLAPYDVY
660 670 680 690 700
HTVRGLRWPV VDGKETLWRY KEGSDPYAKK GSGWDFYGKP DGKALIISAP
710 720 730 740 750
YEAPPESPDA EYDMWLCTGR VLEHWHTGTM TRRVPELYKA VPDAVCYIHP
760 770 780 790 800
EDAKARGLRR GDEVLISNKR GEVRVRVETR GRNRPPQGLV FVPFFDARIL
810 820
INKLILDATD PLSKQTDFKK CPVKITKVA
Length:829
Mass (Da):92,816
Last modified:March 1, 2003 - v1
Checksum:i9AF0DD03181FA6E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016796 Genomic DNA. Translation: AAO07656.1.
RefSeqiNP_762666.1. NC_004460.2.

Genome annotation databases

EnsemblBacteriaiAAO07656; AAO07656; VV2_0721.
GeneIDi1180709.
KEGGivvu:VV2_0721.
PATRICi20164842. VBIVibVul94426_3881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016796 Genomic DNA. Translation: AAO07656.1 .
RefSeqi NP_762666.1. NC_004460.2.

3D structure databases

ProteinModelPortali Q8D623.
SMRi Q8D623. Positions 41-827.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216895.VV2_0721.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO07656 ; AAO07656 ; VV2_0721 .
GeneIDi 1180709.
KEGGi vvu:VV2_0721.
PATRICi 20164842. VBIVibVul94426_3881.

Phylogenomic databases

eggNOGi COG0243.
HOGENOMi HOG000031441.
KOi K02567.
OMAi EYRGKTM.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

BioCyci VVUL216895:GIYM-3557-MONOMER.

Family and domain databases

HAMAPi MF_01630. Nitrate_reduct.
InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
IPR006311. TAT_signal.
[Graphical view ]
Pfami PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01706. NAPA. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiNAPA_VIBVU
AccessioniPrimary (citable) accession number: Q8D623
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3