Q8D5N2 (SYY1_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine--tRNA ligase 1
EC=6.1.1.1

Alternative name(s):
Tyrosyl-tRNA synthetase 1
Short name=TyrRS 1

Gene names

Name:	tyrS1
Ordered Locus Names:	VV2_0878

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity. HAMAP MF_02006
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity. HAMAP MF_02006
Subcellular location	Cytoplasm By similarity HAMAP MF_02006.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Tyrosine--tRNA ligase 1 HAMAP MF_02006		PRO_0000234811
Regions
	Domain	355 – 419	65	S4 RNA-binding
	Motif	41 – 50	10	"HIGH" region HAMAP MF_02006
	Motif	233 – 237	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	36	1	Tyrosine By similarity
	Binding site	173	1	Tyrosine By similarity
	Binding site	177	1	Tyrosine By similarity
	Binding site	236	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D5N2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0C65F0586119F26B	FASTA	422	47,174
	10 20 30 40 50 60 MNSQLLFDDL AQRGLIAQTT DLEQLIALFR QPQTLYCGFD PTAGSLHIGH LVPLIMLKRF 70 80 90 100 110 120 QDAGHQGIAL IGGATGMIGD PSFKASERSL NSAETVAAWV NALATQIQQL MTPHLTQPLV 130 140 150 160 170 180 MVNNADWMQS IGVIAFFRDI GKHFSVNAMI QRESVKQRLA RPDQGISFTE FSYSLLQSYD 190 200 210 220 230 240 FAQLNQTHQC ALQIGGNDQW GNIVSGIDLT RRLNGTNVHG LTLPLITKSD GTKFGKTEGG 250 260 270 280 290 300 AIWLDAAKTS PYMFYQFWLN CDDADVYRFL RYYTFLSVEQ IEQLEATDKA QVGKPSAQRI 310 320 330 340 350 360 LAEEMTRFVH GQAGLESAQR ISQALFSGQL NQLNLAELKQ LEQDGLPCRQ LAHVSDVVTL 370 380 390 400 410 420 LLETGLASSK RQAREWLENG AIRINGERWH ELTLAQTFAL YDQYYIVQRG KKQFAMVKLA QV

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016796 Genomic DNA. Translation: AAO07799.1.
RefSeq	NP_762809.1. NC_004460.2.
3D structure databases
ProteinModelPortal	Q8D5N2.
SMR	Q8D5N2. Positions 1-320.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1180855.
GenomeReviews	Gene locus VV2_0878 in contig AE016796_GR.
KEGG	vvu:VV2_0878.
PATRIC	20165156. VBIVibVul94426_4035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288125.
OMA	TFYIGFD.
ProtClustDB	PRK13354.
Enzyme and pathway databases
BioCyc	VVUL216895:VV2_0878-MONOMER.
Family and domain databases
HAMAP	MF_02006. Tyr_tRNA_synth_type1. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ic. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA-bd. IPR002307. Tyr-tRNA-synth. IPR024088. Tyr-tRNA-synth_bac-type. IPR024107. Tyr-tRNA-synth_bac_1. [Graphical view]
Gene3D	G3DSA:3.10.290.10. G3DSA:3.10.290.10. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01866.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. TyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY1_VIBVU

Accession

Primary (citable) accession number: Q8D5N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program