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Q8D4P0 (GLGB_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:VV2_1252
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length715 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7157151,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188762

Sites

Active site3961Nucleophile By similarity
Active site4491Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D4P0 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 304C61BECF5A5F43

FASTA71581,927
        10         20         30         40         50         60 
MFEKLSQAAC SEPFAFLGPF IDPTQGALRV WMPGATGVAL VLEGQPRIAL EREKESAFIL 

        70         80         90        100        110        120 
KADLNLHLTH YQLAIDWNGV EQLVDDPYQY HGIYAEYDDL HTPKTMYQHM GSQFMTLERD 

       130        140        150        160        170        180 
GKSISGIRFL VYAPHATAVS LVGSFNDWDG RRHPMQRLDY GIWGLFIPDL AEGVSYKFEM 

       190        200        210        220        230        240 
KGPKGEGLPH KADPWGFYAE QYPSFASVTY DHARYQWQDA QWQTRPVTEK RKEALSFYEL 

       250        260        270        280        290        300 
HAGSWKRNEQ GEFLNYRELA AELVPYLVDM GYTHVELMPV SEHPFYGSWG YQPVGLFAPT 

       310        320        330        340        350        360 
SRYGSPDDFK FFVDACHQAG IGVVLDWVPA HFPSDDHGLA NFDGTPLFHD PDPRRGWHQD 

       370        380        390        400        410        420 
WNSFIYDLGR EQVRRFLVSN ALYWFEQFHI DGIRVDAVAS MLYLDYSRSH GQWIPNMDGG 

       430        440        450        460        470        480 
NENYDAIATL KWMNEEVYKY FPNAMTIAEE STAFPGVSAP TFMGGLGFGF KWNMGWMHDS 

       490        500        510        520        530        540 
LSYIKEEPVH RKYHHNTLTF PLVYAHSENY VLSLSHDEVV YGKGSIHNKM PGDEWQQTAN 

       550        560        570        580        590        600 
LRAYFGYMYG QPGKKLNFMG AEIGQTAEWN HDDQLQWFLL DFPRHQGVQA LTRDLNHLYR 

       610        620        630        640        650        660 
NQAALHDQDC IPAGFEWRLQ DAAEQSIIAH ERISEAGERI LVVSNFTPVP RDEFRLGVPN 

       670        680        690        700        710 
KGRYQLLLNT DDSKYAGSGY EVVVDAKSEA VVSEDLAQSI VLRLPPLSTL FYKLV 

« Hide

References

[1]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016796 Genomic DNA. Translation: AAO08146.1.
RefSeqNP_763156.1. NC_004460.2.

3D structure databases

ProteinModelPortalQ8D4P0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216895.VV2_1252.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO08146; AAO08146; VV2_1252.
GeneID1181202.
KEGGvvu:VV2_1252.
PATRIC20165886. VBIVibVul94426_4400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAPIGVAYK.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-4047-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_VIBVU
AccessionPrimary (citable) accession number: Q8D4P0
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries