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Q8D4H3

- BGAL_VIBVU

UniProt

Q8D4H3 - BGAL_VIBVU

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Protein
Beta-galactosidase
Gene
lacZ, VV2_1327
Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Binds 2 magnesium ions per monomer By similarity.UniRule annotation
Binds 1 sodium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001Substrate By similarity
Metal bindingi198 – 1981Sodium By similarity
Binding sitei198 – 1981Substrate By similarity
Sitei354 – 3541Transition state stabilizer By similarity
Sitei388 – 3881Transition state stabilizer By similarity
Metal bindingi413 – 4131Magnesium 1 By similarity
Metal bindingi415 – 4151Magnesium 1 By similarity
Active sitei458 – 4581Proton donor By similarity
Metal bindingi458 – 4581Magnesium 1 By similarity
Binding sitei458 – 4581Substrate By similarity
Active sitei534 – 5341Nucleophile By similarity
Metal bindingi594 – 5941Magnesium 2 By similarity
Metal bindingi598 – 5981Sodium; via carbonyl oxygen By similarity
Metal bindingi601 – 6011Sodium By similarity
Binding sitei601 – 6011Substrate By similarity
Binding sitei1006 – 10061Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-4116-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Short name:
Beta-gal
Alternative name(s):
Lactase
Gene namesi
Name:lacZ
Ordered Locus Names:VV2_1327
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002275: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10321032Beta-galactosidaseUniRule annotation
PRO_0000367012Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV2_1327.

Structurei

3D structure databases

ProteinModelPortaliQ8D4H3.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni534 – 5374Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8D4H3-1 [UniParc]FASTAAdd to Basket

« Hide

MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL     50
NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL QGYDKPIYAN 100
IKYPFDVNPP FVPSDNPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW 150
CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW 200
WLSGIFRDVT LLSKPQHCIE DVFITPELDA CYRDGSLSIV TTIAAPETYQ 250
VQVQLFEGTQ AVTEPNIARP HNRRIDERGT WNDDVVFQTL HLREPKKWTA 300
ETPNLYRLVV SLLDENGTHL ESEAYPVGFR KVEISEGQLK LNGKPLLIRG 350
VNRHEHHPEL GHVMTEEDMI RDICLMKQYN FNAVRTAHYP NHPRWYELCD 400
QYGLYVCDEA NIETHGMQPM SRLSSDPQWA HAYMSRYTQM VLRDKNHPSI 450
IIWSLGNESG HGSNHNAMYA WSKNFDPSRP VQYEGGGSNT TATDIIAPMY 500
ARVNTLVADE AVPKWPIKKW ISLPNETRPL ILCEYAHAMG NSLGSFDEYW 550
AAFREFPRLQ GGFIWDWVDQ GLSQWDENGQ HFWAYGGDFG DEINDRQFCI 600
NGLIFPDRTV HPTLQEAKYC QRMITVSLQE QTQKACTLLV TNENLFRTTD 650
NEQLNWSLLE NGQVIQTGSQ VLSVEADSQT RLEIALNFTP KAQAQYYLNT 700
DICLIEATSW APAGHVVATE QMALRNHAGL AMPTLRTQPA PKLTENGHAI 750
VVSSLDEKHQ WRWDSQSGLL MEWNVDGKAQ MLAAPQDNFF RAPLDNDIGI 800
SEVDNVDPNA WVCRWEMAGI GQWERHCVQC ESETLAHAVV VTTTFAYHFG 850
GDVQAITQWT HTLSNDGEML LDVDVTLADA LPPMPRIGLE LQLPLHQADT 900
PITWQGLGPF ENYPDRLAAA RFGLHTQTLA QMHTPYIFPT DSGLRCGTQW 950
LQVNELAISG DFQFSVSQYA QQQLAEAKHT HDLLAQERIY LRLDHQHMGV 1000
GGDDSWSPSV HKEFQLTEKH YRYQLRFSPA SR 1032
Length:1,032
Mass (Da):117,737
Last modified:March 24, 2009 - v2
Checksum:iC904A3944BDE0D8E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911Q → H in AAK15465. 1 Publication
Sequence conflicti91 – 911Q → H in AAK29750. 1 Publication
Sequence conflicti127 – 1271L → F in AAK15465. 1 Publication
Sequence conflicti127 – 1271L → F in AAK29750. 1 Publication
Sequence conflicti131 – 1333DLL → ELA in AAK15465. 1 Publication
Sequence conflicti131 – 1333DLL → ELA in AAK29750. 1 Publication
Sequence conflicti227 – 2271E → D in AAK15465. 1 Publication
Sequence conflicti227 – 2271E → D in AAK29750. 1 Publication
Sequence conflicti284 – 2841Missing in AAK15465. 1 Publication
Sequence conflicti284 – 2841Missing in AAK29750. 1 Publication
Sequence conflicti382 – 3821N → K in AAK15465. 1 Publication
Sequence conflicti644 – 6441N → K in AAK15465. 1 Publication
Sequence conflicti697 – 6971Y → H in AAK15465. 1 Publication
Sequence conflicti697 – 6971Y → H in AAK29750. 1 Publication
Sequence conflicti745 – 7451E → Q in AAK15465. 1 Publication
Sequence conflicti745 – 7451E → Q in AAK29750. 1 Publication
Sequence conflicti898 – 8981A → T in AAK15465. 1 Publication
Sequence conflicti898 – 8981A → T in AAK29750. 1 Publication
Sequence conflicti1028 – 10281S → K in AAK15465. 1 Publication
Sequence conflicti1028 – 10281S → K in AAK29750. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1.
AY028965 Genomic DNA. Translation: AAK29750.1.
AE016796 Genomic DNA. Translation: AAO08215.2.
RefSeqiNP_763225.2. NC_004460.2.

Genome annotation databases

EnsemblBacteriaiAAO08215; AAO08215; VV2_1327.
GeneIDi1181271.
KEGGivvu:VV2_1327.
PATRICi20166030. VBIVibVul94426_4472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1 .
AY028965 Genomic DNA. Translation: AAK29750.1 .
AE016796 Genomic DNA. Translation: AAO08215.2 .
RefSeqi NP_763225.2. NC_004460.2.

3D structure databases

ProteinModelPortali Q8D4H3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216895.VV2_1327.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO08215 ; AAO08215 ; VV2_1327 .
GeneIDi 1181271.
KEGGi vvu:VV2_1327.
PATRICi 20166030. VBIVibVul94426_4472.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci VVUL216895:GIYM-4116-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-galactosidase of Vibrio vulnificus."
    Kim S.Y., Lee S.E., Morris J.G. Jr., Chung S.S., Rhee J.H.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Beta-galactosidase of Vibrio vulnificus ATCC 29307."
    Baek C.H., Park D.K., Lee K.E., Kim Y.T., You K.H., Hwang W., Kim K.S.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29307 / CDC A8694.
  3. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiBGAL_VIBVU
AccessioniPrimary (citable) accession number: Q8D4H3
Secondary accession number(s): Q9AEQ8, Q9AHK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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