Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8D4H3

- BGAL_VIBVU

UniProt

Q8D4H3 - BGAL_VIBVU

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateUniRule annotation
Metal bindingi198 – 1981SodiumUniRule annotation
Binding sitei198 – 1981SubstrateUniRule annotation
Sitei354 – 3541Transition state stabilizerUniRule annotation
Sitei388 – 3881Transition state stabilizerUniRule annotation
Metal bindingi413 – 4131Magnesium 1UniRule annotation
Metal bindingi415 – 4151Magnesium 1UniRule annotation
Active sitei458 – 4581Proton donorUniRule annotation
Metal bindingi458 – 4581Magnesium 1UniRule annotation
Binding sitei458 – 4581SubstrateUniRule annotation
Active sitei534 – 5341NucleophileUniRule annotation
Metal bindingi594 – 5941Magnesium 2UniRule annotation
Metal bindingi598 – 5981Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi601 – 6011SodiumUniRule annotation
Binding sitei601 – 6011SubstrateUniRule annotation
Binding sitei1006 – 10061SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Enzyme and pathway databases

BioCyciVVUL216895:GIYM-4116-MONOMER.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VV2_1327
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
ProteomesiUP000002275: Chromosome 2

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10321032Beta-galactosidasePRO_0000367012Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV2_1327.

Structurei

3D structure databases

ProteinModelPortaliQ8D4H3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni534 – 5374Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8D4H3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL
60 70 80 90 100
NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL QGYDKPIYAN
110 120 130 140 150
IKYPFDVNPP FVPSDNPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW
160 170 180 190 200
CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW
210 220 230 240 250
WLSGIFRDVT LLSKPQHCIE DVFITPELDA CYRDGSLSIV TTIAAPETYQ
260 270 280 290 300
VQVQLFEGTQ AVTEPNIARP HNRRIDERGT WNDDVVFQTL HLREPKKWTA
310 320 330 340 350
ETPNLYRLVV SLLDENGTHL ESEAYPVGFR KVEISEGQLK LNGKPLLIRG
360 370 380 390 400
VNRHEHHPEL GHVMTEEDMI RDICLMKQYN FNAVRTAHYP NHPRWYELCD
410 420 430 440 450
QYGLYVCDEA NIETHGMQPM SRLSSDPQWA HAYMSRYTQM VLRDKNHPSI
460 470 480 490 500
IIWSLGNESG HGSNHNAMYA WSKNFDPSRP VQYEGGGSNT TATDIIAPMY
510 520 530 540 550
ARVNTLVADE AVPKWPIKKW ISLPNETRPL ILCEYAHAMG NSLGSFDEYW
560 570 580 590 600
AAFREFPRLQ GGFIWDWVDQ GLSQWDENGQ HFWAYGGDFG DEINDRQFCI
610 620 630 640 650
NGLIFPDRTV HPTLQEAKYC QRMITVSLQE QTQKACTLLV TNENLFRTTD
660 670 680 690 700
NEQLNWSLLE NGQVIQTGSQ VLSVEADSQT RLEIALNFTP KAQAQYYLNT
710 720 730 740 750
DICLIEATSW APAGHVVATE QMALRNHAGL AMPTLRTQPA PKLTENGHAI
760 770 780 790 800
VVSSLDEKHQ WRWDSQSGLL MEWNVDGKAQ MLAAPQDNFF RAPLDNDIGI
810 820 830 840 850
SEVDNVDPNA WVCRWEMAGI GQWERHCVQC ESETLAHAVV VTTTFAYHFG
860 870 880 890 900
GDVQAITQWT HTLSNDGEML LDVDVTLADA LPPMPRIGLE LQLPLHQADT
910 920 930 940 950
PITWQGLGPF ENYPDRLAAA RFGLHTQTLA QMHTPYIFPT DSGLRCGTQW
960 970 980 990 1000
LQVNELAISG DFQFSVSQYA QQQLAEAKHT HDLLAQERIY LRLDHQHMGV
1010 1020 1030
GGDDSWSPSV HKEFQLTEKH YRYQLRFSPA SR
Length:1,032
Mass (Da):117,737
Last modified:March 24, 2009 - v2
Checksum:iC904A3944BDE0D8E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti91 – 911Q → H in AAK15465. 1 PublicationCurated
Sequence conflicti91 – 911Q → H in AAK29750. 1 PublicationCurated
Sequence conflicti127 – 1271L → F in AAK15465. 1 PublicationCurated
Sequence conflicti127 – 1271L → F in AAK29750. 1 PublicationCurated
Sequence conflicti131 – 1333DLL → ELA in AAK15465. 1 PublicationCurated
Sequence conflicti131 – 1333DLL → ELA in AAK29750. 1 PublicationCurated
Sequence conflicti227 – 2271E → D in AAK15465. 1 PublicationCurated
Sequence conflicti227 – 2271E → D in AAK29750. 1 PublicationCurated
Sequence conflicti284 – 2841Missing in AAK15465. 1 PublicationCurated
Sequence conflicti284 – 2841Missing in AAK29750. 1 PublicationCurated
Sequence conflicti382 – 3821N → K in AAK15465. 1 PublicationCurated
Sequence conflicti644 – 6441N → K in AAK15465. 1 PublicationCurated
Sequence conflicti697 – 6971Y → H in AAK15465. 1 PublicationCurated
Sequence conflicti697 – 6971Y → H in AAK29750. 1 PublicationCurated
Sequence conflicti745 – 7451E → Q in AAK15465. 1 PublicationCurated
Sequence conflicti745 – 7451E → Q in AAK29750. 1 PublicationCurated
Sequence conflicti898 – 8981A → T in AAK15465. 1 PublicationCurated
Sequence conflicti898 – 8981A → T in AAK29750. 1 PublicationCurated
Sequence conflicti1028 – 10281S → K in AAK15465. 1 PublicationCurated
Sequence conflicti1028 – 10281S → K in AAK29750. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1.
AY028965 Genomic DNA. Translation: AAK29750.1.
AE016796 Genomic DNA. Translation: AAO08215.2.
RefSeqiNP_763225.2. NC_004460.2.
WP_011082210.1. NC_004460.2.

Genome annotation databases

EnsemblBacteriaiAAO08215; AAO08215; VV2_1327.
GeneIDi1181271.
KEGGivvu:VV2_1327.
PATRICi20166030. VBIVibVul94426_4472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1 .
AY028965 Genomic DNA. Translation: AAK29750.1 .
AE016796 Genomic DNA. Translation: AAO08215.2 .
RefSeqi NP_763225.2. NC_004460.2.
WP_011082210.1. NC_004460.2.

3D structure databases

ProteinModelPortali Q8D4H3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 216895.VV2_1327.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO08215 ; AAO08215 ; VV2_1327 .
GeneIDi 1181271.
KEGGi vvu:VV2_1327.
PATRICi 20166030. VBIVibVul94426_4472.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Enzyme and pathway databases

BioCyci VVUL216895:GIYM-4116-MONOMER.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Beta-galactosidase of Vibrio vulnificus."
    Kim S.Y., Lee S.E., Morris J.G. Jr., Chung S.S., Rhee J.H.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Beta-galactosidase of Vibrio vulnificus ATCC 29307."
    Baek C.H., Park D.K., Lee K.E., Kim Y.T., You K.H., Hwang W., Kim K.S.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 29307 / CDC A8694.
  3. "Complete genome sequence of Vibrio vulnificus CMCP6."
    Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CMCP6.

Entry informationi

Entry nameiBGAL_VIBVU
AccessioniPrimary (citable) accession number: Q8D4H3
Secondary accession number(s): Q9AEQ8, Q9AHK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: November 26, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3