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Q8D4H3

- BGAL_VIBVU

UniProt

Q8D4H3 - BGAL_VIBVU

Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 2 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001SubstrateUniRule annotation
    Metal bindingi198 – 1981SodiumUniRule annotation
    Binding sitei198 – 1981SubstrateUniRule annotation
    Sitei354 – 3541Transition state stabilizerUniRule annotation
    Sitei388 – 3881Transition state stabilizerUniRule annotation
    Metal bindingi413 – 4131Magnesium 1UniRule annotation
    Metal bindingi415 – 4151Magnesium 1UniRule annotation
    Active sitei458 – 4581Proton donorUniRule annotation
    Metal bindingi458 – 4581Magnesium 1UniRule annotation
    Binding sitei458 – 4581SubstrateUniRule annotation
    Active sitei534 – 5341NucleophileUniRule annotation
    Metal bindingi594 – 5941Magnesium 2UniRule annotation
    Metal bindingi598 – 5981Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi601 – 6011SodiumUniRule annotation
    Binding sitei601 – 6011SubstrateUniRule annotation
    Binding sitei1006 – 10061SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Enzyme and pathway databases

    BioCyciVVUL216895:GIYM-4116-MONOMER.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:VV2_1327
    OrganismiVibrio vulnificus (strain CMCP6)
    Taxonomic identifieri216895 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
    ProteomesiUP000002275: Chromosome 2

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10321032Beta-galactosidasePRO_0000367012Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi216895.VV2_1327.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8D4H3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni534 – 5374Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiDPKKPYI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8D4H3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL     50
    NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL QGYDKPIYAN 100
    IKYPFDVNPP FVPSDNPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW 150
    CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW 200
    WLSGIFRDVT LLSKPQHCIE DVFITPELDA CYRDGSLSIV TTIAAPETYQ 250
    VQVQLFEGTQ AVTEPNIARP HNRRIDERGT WNDDVVFQTL HLREPKKWTA 300
    ETPNLYRLVV SLLDENGTHL ESEAYPVGFR KVEISEGQLK LNGKPLLIRG 350
    VNRHEHHPEL GHVMTEEDMI RDICLMKQYN FNAVRTAHYP NHPRWYELCD 400
    QYGLYVCDEA NIETHGMQPM SRLSSDPQWA HAYMSRYTQM VLRDKNHPSI 450
    IIWSLGNESG HGSNHNAMYA WSKNFDPSRP VQYEGGGSNT TATDIIAPMY 500
    ARVNTLVADE AVPKWPIKKW ISLPNETRPL ILCEYAHAMG NSLGSFDEYW 550
    AAFREFPRLQ GGFIWDWVDQ GLSQWDENGQ HFWAYGGDFG DEINDRQFCI 600
    NGLIFPDRTV HPTLQEAKYC QRMITVSLQE QTQKACTLLV TNENLFRTTD 650
    NEQLNWSLLE NGQVIQTGSQ VLSVEADSQT RLEIALNFTP KAQAQYYLNT 700
    DICLIEATSW APAGHVVATE QMALRNHAGL AMPTLRTQPA PKLTENGHAI 750
    VVSSLDEKHQ WRWDSQSGLL MEWNVDGKAQ MLAAPQDNFF RAPLDNDIGI 800
    SEVDNVDPNA WVCRWEMAGI GQWERHCVQC ESETLAHAVV VTTTFAYHFG 850
    GDVQAITQWT HTLSNDGEML LDVDVTLADA LPPMPRIGLE LQLPLHQADT 900
    PITWQGLGPF ENYPDRLAAA RFGLHTQTLA QMHTPYIFPT DSGLRCGTQW 950
    LQVNELAISG DFQFSVSQYA QQQLAEAKHT HDLLAQERIY LRLDHQHMGV 1000
    GGDDSWSPSV HKEFQLTEKH YRYQLRFSPA SR 1032
    Length:1,032
    Mass (Da):117,737
    Last modified:March 24, 2009 - v2
    Checksum:iC904A3944BDE0D8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti91 – 911Q → H in AAK15465. 1 PublicationCurated
    Sequence conflicti91 – 911Q → H in AAK29750. 1 PublicationCurated
    Sequence conflicti127 – 1271L → F in AAK15465. 1 PublicationCurated
    Sequence conflicti127 – 1271L → F in AAK29750. 1 PublicationCurated
    Sequence conflicti131 – 1333DLL → ELA in AAK15465. 1 PublicationCurated
    Sequence conflicti131 – 1333DLL → ELA in AAK29750. 1 PublicationCurated
    Sequence conflicti227 – 2271E → D in AAK15465. 1 PublicationCurated
    Sequence conflicti227 – 2271E → D in AAK29750. 1 PublicationCurated
    Sequence conflicti284 – 2841Missing in AAK15465. 1 PublicationCurated
    Sequence conflicti284 – 2841Missing in AAK29750. 1 PublicationCurated
    Sequence conflicti382 – 3821N → K in AAK15465. 1 PublicationCurated
    Sequence conflicti644 – 6441N → K in AAK15465. 1 PublicationCurated
    Sequence conflicti697 – 6971Y → H in AAK15465. 1 PublicationCurated
    Sequence conflicti697 – 6971Y → H in AAK29750. 1 PublicationCurated
    Sequence conflicti745 – 7451E → Q in AAK15465. 1 PublicationCurated
    Sequence conflicti745 – 7451E → Q in AAK29750. 1 PublicationCurated
    Sequence conflicti898 – 8981A → T in AAK15465. 1 PublicationCurated
    Sequence conflicti898 – 8981A → T in AAK29750. 1 PublicationCurated
    Sequence conflicti1028 – 10281S → K in AAK15465. 1 PublicationCurated
    Sequence conflicti1028 – 10281S → K in AAK29750. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305636 Genomic DNA. Translation: AAK15465.1.
    AY028965 Genomic DNA. Translation: AAK29750.1.
    AE016796 Genomic DNA. Translation: AAO08215.2.
    RefSeqiNP_763225.2. NC_004460.2.

    Genome annotation databases

    EnsemblBacteriaiAAO08215; AAO08215; VV2_1327.
    GeneIDi1181271.
    KEGGivvu:VV2_1327.
    PATRICi20166030. VBIVibVul94426_4472.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF305636 Genomic DNA. Translation: AAK15465.1 .
    AY028965 Genomic DNA. Translation: AAK29750.1 .
    AE016796 Genomic DNA. Translation: AAO08215.2 .
    RefSeqi NP_763225.2. NC_004460.2.

    3D structure databases

    ProteinModelPortali Q8D4H3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 216895.VV2_1327.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO08215 ; AAO08215 ; VV2_1327 .
    GeneIDi 1181271.
    KEGGi vvu:VV2_1327.
    PATRICi 20166030. VBIVibVul94426_4472.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi DPKKPYI.
    OrthoDBi EOG6XWV0T.

    Enzyme and pathway databases

    BioCyci VVUL216895:GIYM-4116-MONOMER.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-galactosidase of Vibrio vulnificus."
      Kim S.Y., Lee S.E., Morris J.G. Jr., Chung S.S., Rhee J.H.
      Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Beta-galactosidase of Vibrio vulnificus ATCC 29307."
      Baek C.H., Park D.K., Lee K.E., Kim Y.T., You K.H., Hwang W., Kim K.S.
      Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29307 / CDC A8694.
    3. "Complete genome sequence of Vibrio vulnificus CMCP6."
      Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
      Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CMCP6.

    Entry informationi

    Entry nameiBGAL_VIBVU
    AccessioniPrimary (citable) accession number: Q8D4H3
    Secondary accession number(s): Q9AEQ8, Q9AHK2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 75 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3