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Protein

Beta-galactosidase

Gene

lacZ

Organism
Vibrio vulnificus (strain CMCP6)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na+UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateUniRule annotation1
Metal bindingi198SodiumUniRule annotation1
Binding sitei198SubstrateUniRule annotation1
Sitei354Transition state stabilizerUniRule annotation1
Sitei388Transition state stabilizerUniRule annotation1
Metal bindingi413Magnesium 1UniRule annotation1
Metal bindingi415Magnesium 1UniRule annotation1
Active sitei458Proton donorUniRule annotation1
Metal bindingi458Magnesium 1UniRule annotation1
Binding sitei458SubstrateUniRule annotation1
Active sitei534NucleophileUniRule annotation1
Metal bindingi594Magnesium 2UniRule annotation1
Metal bindingi598Sodium; via carbonyl oxygenUniRule annotation1
Metal bindingi601SodiumUniRule annotation1
Binding sitei601SubstrateUniRule annotation1
Binding sitei1006SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:VV2_1327
OrganismiVibrio vulnificus (strain CMCP6)
Taxonomic identifieri216895 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003670121 – 1032Beta-galactosidaseAdd BLAST1032

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi216895.VV2_1327.

Structurei

3D structure databases

ProteinModelPortaliQ8D4H3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni534 – 537Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDIETRMY.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8D4H3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL
60 70 80 90 100
NGQWKFKLFD APEQVDGQFT QADFNDAEWD EIPVPSNWQL QGYDKPIYAN
110 120 130 140 150
IKYPFDVNPP FVPSDNPTGC YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW
160 170 180 190 200
CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT LAVMVMRWCD GSYLEDQDMW
210 220 230 240 250
WLSGIFRDVT LLSKPQHCIE DVFITPELDA CYRDGSLSIV TTIAAPETYQ
260 270 280 290 300
VQVQLFEGTQ AVTEPNIARP HNRRIDERGT WNDDVVFQTL HLREPKKWTA
310 320 330 340 350
ETPNLYRLVV SLLDENGTHL ESEAYPVGFR KVEISEGQLK LNGKPLLIRG
360 370 380 390 400
VNRHEHHPEL GHVMTEEDMI RDICLMKQYN FNAVRTAHYP NHPRWYELCD
410 420 430 440 450
QYGLYVCDEA NIETHGMQPM SRLSSDPQWA HAYMSRYTQM VLRDKNHPSI
460 470 480 490 500
IIWSLGNESG HGSNHNAMYA WSKNFDPSRP VQYEGGGSNT TATDIIAPMY
510 520 530 540 550
ARVNTLVADE AVPKWPIKKW ISLPNETRPL ILCEYAHAMG NSLGSFDEYW
560 570 580 590 600
AAFREFPRLQ GGFIWDWVDQ GLSQWDENGQ HFWAYGGDFG DEINDRQFCI
610 620 630 640 650
NGLIFPDRTV HPTLQEAKYC QRMITVSLQE QTQKACTLLV TNENLFRTTD
660 670 680 690 700
NEQLNWSLLE NGQVIQTGSQ VLSVEADSQT RLEIALNFTP KAQAQYYLNT
710 720 730 740 750
DICLIEATSW APAGHVVATE QMALRNHAGL AMPTLRTQPA PKLTENGHAI
760 770 780 790 800
VVSSLDEKHQ WRWDSQSGLL MEWNVDGKAQ MLAAPQDNFF RAPLDNDIGI
810 820 830 840 850
SEVDNVDPNA WVCRWEMAGI GQWERHCVQC ESETLAHAVV VTTTFAYHFG
860 870 880 890 900
GDVQAITQWT HTLSNDGEML LDVDVTLADA LPPMPRIGLE LQLPLHQADT
910 920 930 940 950
PITWQGLGPF ENYPDRLAAA RFGLHTQTLA QMHTPYIFPT DSGLRCGTQW
960 970 980 990 1000
LQVNELAISG DFQFSVSQYA QQQLAEAKHT HDLLAQERIY LRLDHQHMGV
1010 1020 1030
GGDDSWSPSV HKEFQLTEKH YRYQLRFSPA SR
Length:1,032
Mass (Da):117,737
Last modified:March 24, 2009 - v2
Checksum:iC904A3944BDE0D8E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti91Q → H in AAK15465 (Ref. 1) Curated1
Sequence conflicti91Q → H in AAK29750 (Ref. 2) Curated1
Sequence conflicti127L → F in AAK15465 (Ref. 1) Curated1
Sequence conflicti127L → F in AAK29750 (Ref. 2) Curated1
Sequence conflicti131 – 133DLL → ELA in AAK15465 (Ref. 1) Curated3
Sequence conflicti131 – 133DLL → ELA in AAK29750 (Ref. 2) Curated3
Sequence conflicti227E → D in AAK15465 (Ref. 1) Curated1
Sequence conflicti227E → D in AAK29750 (Ref. 2) Curated1
Sequence conflicti284Missing in AAK15465 (Ref. 1) Curated1
Sequence conflicti284Missing in AAK29750 (Ref. 2) Curated1
Sequence conflicti382N → K in AAK15465 (Ref. 1) Curated1
Sequence conflicti644N → K in AAK15465 (Ref. 1) Curated1
Sequence conflicti697Y → H in AAK15465 (Ref. 1) Curated1
Sequence conflicti697Y → H in AAK29750 (Ref. 2) Curated1
Sequence conflicti745E → Q in AAK15465 (Ref. 1) Curated1
Sequence conflicti745E → Q in AAK29750 (Ref. 2) Curated1
Sequence conflicti898A → T in AAK15465 (Ref. 1) Curated1
Sequence conflicti898A → T in AAK29750 (Ref. 2) Curated1
Sequence conflicti1028S → K in AAK15465 (Ref. 1) Curated1
Sequence conflicti1028S → K in AAK29750 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1.
AY028965 Genomic DNA. Translation: AAK29750.1.
AE016796 Genomic DNA. Translation: AAO08215.2.
RefSeqiWP_011082210.1. NC_004460.2.

Genome annotation databases

EnsemblBacteriaiAAO08215; AAO08215; VV2_1327.
KEGGivvu:VV2_1327.
PATRICi20166030. VBIVibVul94426_4472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF305636 Genomic DNA. Translation: AAK15465.1.
AY028965 Genomic DNA. Translation: AAK29750.1.
AE016796 Genomic DNA. Translation: AAO08215.2.
RefSeqiWP_011082210.1. NC_004460.2.

3D structure databases

ProteinModelPortaliQ8D4H3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi216895.VV2_1327.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO08215; AAO08215; VV2_1327.
KEGGivvu:VV2_1327.
PATRICi20166030. VBIVibVul94426_4472.

Phylogenomic databases

eggNOGiENOG4105CNT. Bacteria.
COG3250. LUCA.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDIETRMY.
OrthoDBiPOG091H07QB.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal. 1 hit.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. GH-type_carb-bd.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006103. Glyco_hydro_2_cat.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR032312. LacZ_4.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF16353. DUF4981. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_VIBVU
AccessioniPrimary (citable) accession number: Q8D4H3
Secondary accession number(s): Q9AEQ8, Q9AHK2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.