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Q8D4H3 (BGAL_VIBVU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:VV2_1327
OrganismVibrio vulnificus (strain CMCP6) [Complete proteome] [HAMAP]
Taxonomic identifier216895 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio

Protein attributes

Sequence length1032 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10321032Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367012

Regions

Region534 – 5374Substrate binding By similarity

Sites

Active site4581Proton donor By similarity
Active site5341Nucleophile By similarity
Metal binding1981Sodium By similarity
Metal binding4131Magnesium 1 By similarity
Metal binding4151Magnesium 1 By similarity
Metal binding4581Magnesium 1 By similarity
Metal binding5941Magnesium 2 By similarity
Metal binding5981Sodium; via carbonyl oxygen By similarity
Metal binding6011Sodium By similarity
Binding site1001Substrate By similarity
Binding site1981Substrate By similarity
Binding site4581Substrate By similarity
Binding site6011Substrate By similarity
Binding site10061Substrate By similarity
Site3541Transition state stabilizer By similarity
Site3881Transition state stabilizer By similarity

Experimental info

Sequence conflict911Q → H in AAK15465. Ref.1
Sequence conflict911Q → H in AAK29750. Ref.2
Sequence conflict1271L → F in AAK15465. Ref.1
Sequence conflict1271L → F in AAK29750. Ref.2
Sequence conflict131 – 1333DLL → ELA in AAK15465. Ref.1
Sequence conflict131 – 1333DLL → ELA in AAK29750. Ref.2
Sequence conflict2271E → D in AAK15465. Ref.1
Sequence conflict2271E → D in AAK29750. Ref.2
Sequence conflict2841Missing in AAK15465. Ref.1
Sequence conflict2841Missing in AAK29750. Ref.2
Sequence conflict3821N → K in AAK15465. Ref.1
Sequence conflict6441N → K in AAK15465. Ref.1
Sequence conflict6971Y → H in AAK15465. Ref.1
Sequence conflict6971Y → H in AAK29750. Ref.2
Sequence conflict7451E → Q in AAK15465. Ref.1
Sequence conflict7451E → Q in AAK29750. Ref.2
Sequence conflict8981A → T in AAK15465. Ref.1
Sequence conflict8981A → T in AAK29750. Ref.2
Sequence conflict10281S → K in AAK15465. Ref.1
Sequence conflict10281S → K in AAK29750. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q8D4H3 [UniParc].

Last modified March 24, 2009. Version 2.
Checksum: C904A3944BDE0D8E

FASTA1,032117,737
        10         20         30         40         50         60 
MTAFSEILQR RDWENPQSVN IHCLKAHSPL ASFRDMAHAR DGIHAQRQSL NGQWKFKLFD 

        70         80         90        100        110        120 
APEQVDGQFT QADFNDAEWD EIPVPSNWQL QGYDKPIYAN IKYPFDVNPP FVPSDNPTGC 

       130        140        150        160        170        180 
YRTRVSLSPE DLLNTQRIIF DGVNSAFHLW CNGTWVGYSQ DSRLPAEFDL TSHLVAGENT 

       190        200        210        220        230        240 
LAVMVMRWCD GSYLEDQDMW WLSGIFRDVT LLSKPQHCIE DVFITPELDA CYRDGSLSIV 

       250        260        270        280        290        300 
TTIAAPETYQ VQVQLFEGTQ AVTEPNIARP HNRRIDERGT WNDDVVFQTL HLREPKKWTA 

       310        320        330        340        350        360 
ETPNLYRLVV SLLDENGTHL ESEAYPVGFR KVEISEGQLK LNGKPLLIRG VNRHEHHPEL 

       370        380        390        400        410        420 
GHVMTEEDMI RDICLMKQYN FNAVRTAHYP NHPRWYELCD QYGLYVCDEA NIETHGMQPM 

       430        440        450        460        470        480 
SRLSSDPQWA HAYMSRYTQM VLRDKNHPSI IIWSLGNESG HGSNHNAMYA WSKNFDPSRP 

       490        500        510        520        530        540 
VQYEGGGSNT TATDIIAPMY ARVNTLVADE AVPKWPIKKW ISLPNETRPL ILCEYAHAMG 

       550        560        570        580        590        600 
NSLGSFDEYW AAFREFPRLQ GGFIWDWVDQ GLSQWDENGQ HFWAYGGDFG DEINDRQFCI 

       610        620        630        640        650        660 
NGLIFPDRTV HPTLQEAKYC QRMITVSLQE QTQKACTLLV TNENLFRTTD NEQLNWSLLE 

       670        680        690        700        710        720 
NGQVIQTGSQ VLSVEADSQT RLEIALNFTP KAQAQYYLNT DICLIEATSW APAGHVVATE 

       730        740        750        760        770        780 
QMALRNHAGL AMPTLRTQPA PKLTENGHAI VVSSLDEKHQ WRWDSQSGLL MEWNVDGKAQ 

       790        800        810        820        830        840 
MLAAPQDNFF RAPLDNDIGI SEVDNVDPNA WVCRWEMAGI GQWERHCVQC ESETLAHAVV 

       850        860        870        880        890        900 
VTTTFAYHFG GDVQAITQWT HTLSNDGEML LDVDVTLADA LPPMPRIGLE LQLPLHQADT 

       910        920        930        940        950        960 
PITWQGLGPF ENYPDRLAAA RFGLHTQTLA QMHTPYIFPT DSGLRCGTQW LQVNELAISG 

       970        980        990       1000       1010       1020 
DFQFSVSQYA QQQLAEAKHT HDLLAQERIY LRLDHQHMGV GGDDSWSPSV HKEFQLTEKH 

      1030 
YRYQLRFSPA SR 

« Hide

References

« Hide 'large scale' references
[1]"Beta-galactosidase of Vibrio vulnificus."
Kim S.Y., Lee S.E., Morris J.G. Jr., Chung S.S., Rhee J.H.
Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Beta-galactosidase of Vibrio vulnificus ATCC 29307."
Baek C.H., Park D.K., Lee K.E., Kim Y.T., You K.H., Hwang W., Kim K.S.
Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29307 / CDC A8694.
[3]"Complete genome sequence of Vibrio vulnificus CMCP6."
Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.
Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CMCP6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF305636 Genomic DNA. Translation: AAK15465.1.
AY028965 Genomic DNA. Translation: AAK29750.1.
AE016796 Genomic DNA. Translation: AAO08215.2.
RefSeqNP_763225.2. NC_004460.2.

3D structure databases

ProteinModelPortalQ8D4H3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING216895.VV2_1327.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO08215; AAO08215; VV2_1327.
GeneID1181271.
KEGGvvu:VV2_1327.
PATRIC20166030. VBIVibVul94426_4472.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMADPKKPYI.
OrthoDBEOG6XWV0T.

Enzyme and pathway databases

BioCycVVUL216895:GIYM-4116-MONOMER.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_VIBVU
AccessionPrimary (citable) accession number: Q8D4H3
Secondary accession number(s): Q9AEQ8, Q9AHK2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 14, 2014
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries