Q8D3I2 (PDXA_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase
EC=1.1.1.262

Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase

Gene names

Name:	pdxA
Ordered Locus Names:	WIGBR0190

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia › Wigglesworthia glossinidia

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536
Catalytic activity	4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536
Cofactor	Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_00536
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00536.
Miscellaneous	The active site is located at the dimer interface By similarity. HAMAP-Rule MF_00536
Sequence similarities	Belongs to the PdxA family.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Magnesium Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	pyridoxal phosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4-hydroxythreonine-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD binding Inferred from electronic annotation. Source: InterPro cobalt ion binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 332	332	4-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536		PRO_1000051525
Sites
	Metal binding	166	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	211	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	266	1	Divalent metal cation; shared with dimeric partner By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	292	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D3I2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CF99243B415D3991	FASTA	332	36,925
	10 20 30 40 50 60 MNKISRIIIT PGEPAGIGFD LVVLISQKIW RSELVICCDP DILVKRAKDH GIKLKLHFYN 70 80 90 100 110 120 KNLSPKVREK GELTIIPIKV NSNVTRGILN INNSYYVINT LTRACLGCIN KEFSALVTGP 130 140 150 160 170 180 VHKGIIKDSG IKFIGHTEFL AGFSLCKNPV MMLMYNSLRI ALATNHIPIK DISKCINFNL 190 200 210 220 230 240 IRKKLIVIFN SLRNIFKINN PCIYVCGLNP HAGESGHIGT EEINIIQPAI SSLKYLPCKI 250 260 270 280 290 300 IGPISADSIF QQKYLKDADA ILAMYHDQGL PMIKYIGFRK SVNITLGLPF IRTSVDHGTA 310 320 330 LNIKNLNEIS SKSMEKAISL AIDISQDNSI KK

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24165.1.
RefSeq	NP_871022.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D3I2.
SMR	Q8D3I2. Positions 5-327.
ModBase	Search...
Protein-protein interaction databases
STRING	36870.WGLp019.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC24165; BAC24165; BAC24165.
GeneID	1257475.
KEGG	wbr:WGLp019.
PATRIC	24024153. VBIWigGlo15804_0029.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1995.
KO	K00097.
OMA	AYVAMYH.
Enzyme and pathway databases
UniPathway	UPA00244; UER00312.
Family and domain databases
Gene3D	3.40.718.10. 1 hit.
HAMAP	MF_00536. PdxA.
InterPro	IPR024084. IsoPropMal-DH-like_dom. IPR005255. PdxA. [Graphical view]
Pfam	PF04166. PdxA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00557. pdxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXA_WIGBR

Accession

Primary (citable) accession number: Q8D3I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program