Q8D3C3 (NTPA_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

WIGBR0780

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia

Protein attributes

Sequence length	199 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 199	199	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178264
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	68 – 69	2	Substrate binding By similarity
Sites
	Metal binding	40	1	Magnesium or manganese By similarity
	Metal binding	68	1	Magnesium or manganese By similarity
	Binding site	157	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Binding site	183	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D3C3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B97FCA79A52E5AA5	FASTA	199	22,534
	10 20 30 40 50 60 MKKIILATSN KNKIIEFKKI LSELNINTIS QKDLGICSIE ENKSTFLENA LIKARNASKY 70 80 90 100 110 120 GFPALSDDSG LIIKTLNGEP GVYSSRFSGN QSNDIKNINM VLKKMLPFKK MDRQACMHCV 130 140 150 160 170 180 LIYIRNPNDP IPIISSGTIY GKISNSISKI NFGFGYDSIF FLPKKKKTIS ELTLEEKIKI 190 SHRGIAMKKM IKFLKNEKY

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed: 12219091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24224.1.
RefSeq	NP_871081.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D3C3.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8D3C3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1257512.
GenomeReviews	Gene locus WIGBR0780 in contig BA000021_GR.
KEGG	wbr:WGLp078.
PATRIC	24024277. VBIWigGlo15804_0091.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	ESSTRLT.
PhylomeDB	Q8D3C3.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_WIGBR

Accession

Primary (citable) accession number: Q8D3C3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program