Q8D335 (GSHB_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	WIGBR1660

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia › Wigglesworthia glossinidia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence BAC24312.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197495
Regions
	Domain	123 – 309	187	ATP-grasp
	Nucleotide binding	149 – 206	58	ATP By similarity
Sites
	Metal binding	280	1	Magnesium or manganese By similarity
	Metal binding	282	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D335 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: B54026AC2A0E7F44	FASTA	316	36,507
	10 20 30 40 50 60 MIKLGIIMDP ISSIKIKKDT SFSIMLEAQK RNYFLYYIEM KDIYYEVGEV YANSYLISVK 70 80 90 100 110 120 YDEKKWYSFK KKYTINLKEL DVILMRKDPP FDIEFLYITY ILEHIENFGV LIINKPKSLR 130 140 150 160 170 180 DYNEKISTLS FKYSPKTLIS CSKKAIYSFQ EKFGDIILKP INKMGGDSVF YVKKNDPNVS 190 200 210 220 230 240 VIIDQLTNYG NSFCLIQEYI KEILNGDRRI IMINGSPLPY CLVRIPNDKE IRGNLAAGAS 250 260 270 280 290 300 FDILPLRKID YEISNNISSF LKDKGLIFVG LDIIGNYLTE INITSPTGIN EIESVYKVSI 310 SGILLDSIEK LLNIKH

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24312.1. Different initiation.
RefSeq	NP_871169.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D335.
ModBase	Search...
Protein-protein interaction databases
STRING	36870.WGLp166.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC24312; BAC24312; BAC24312.
GeneID	1256961.
KEGG	wbr:WGLp166.
PATRIC	24024487. VBIWigGlo15804_0186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0189.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_WIGBR

Accession

Primary (citable) accession number: Q8D335

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	January 16, 2004
Last modified:	May 1, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program