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Q8D335 (GSHB_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:WIGBR1660
OrganismWigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]
Taxonomic identifier36870 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence BAC24312.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197495

Regions

Domain123 – 309187ATP-grasp
Nucleotide binding149 – 20658ATP By similarity

Sites

Metal binding2801Magnesium or manganese By similarity
Metal binding2821Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D335 [UniParc].

Last modified January 16, 2004. Version 2.
Checksum: B54026AC2A0E7F44

FASTA31636,507
        10         20         30         40         50         60 
MIKLGIIMDP ISSIKIKKDT SFSIMLEAQK RNYFLYYIEM KDIYYEVGEV YANSYLISVK 

        70         80         90        100        110        120 
YDEKKWYSFK KKYTINLKEL DVILMRKDPP FDIEFLYITY ILEHIENFGV LIINKPKSLR 

       130        140        150        160        170        180 
DYNEKISTLS FKYSPKTLIS CSKKAIYSFQ EKFGDIILKP INKMGGDSVF YVKKNDPNVS 

       190        200        210        220        230        240 
VIIDQLTNYG NSFCLIQEYI KEILNGDRRI IMINGSPLPY CLVRIPNDKE IRGNLAAGAS 

       250        260        270        280        290        300 
FDILPLRKID YEISNNISSF LKDKGLIFVG LDIIGNYLTE INITSPTGIN EIESVYKVSI 

       310 
SGILLDSIEK LLNIKH 

« Hide

References

[1]"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000021 Genomic DNA. Translation: BAC24312.1. Different initiation.
RefSeqNP_871169.1. NC_004344.2.

3D structure databases

ProteinModelPortalQ8D335.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36870.WGLp166.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC24312; BAC24312; BAC24312.
GeneID1256961.
KEGGwbr:WGLp166.
PATRIC24024487. VBIWigGlo15804_0186.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMAPTCFQEI.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_WIGBR
AccessionPrimary (citable) accession number: Q8D335
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: January 16, 2004
Last modified: February 19, 2014
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways