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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei622 – 6221ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:WIGBR1680
OrganismiWigglesworthia glossinidia brevipalpis
Taxonomic identifieri36870 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia
ProteomesiUP000000562 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 864864Leucine--tRNA ligasePRO_0000152117Add
BLAST

Proteomic databases

PRIDEiQ8D333.

Interactioni

Protein-protein interaction databases

STRINGi36870.WGLp168.

Structurei

3D structure databases

ProteinModelPortaliQ8D333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi619 – 6235"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8D333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKEYSCKEI ERFVQKHWEI NDTFKVLEDS KKDKYYCVSM MPYPSGKLHM
60 70 80 90 100
GHVRNYVLGD VIARYQRMLG KNVLHPIGWD AFGLPAETAA INNKISPEKW
110 120 130 140 150
TISSIEYMKN QLKLLGCSYD WSREIITCDP KYYKWEQLLF TKLYNKNKAY
160 170 180 190 200
KKKSIVNWCP KDKTVLANEQ VIDNLCWRCS SNIEMKKIFQ WFIKITDYAD
210 220 230 240 250
ELLNDLNDLK LWPKKVKVMQ RNWIGKSKGI DVLFHIKDTN EKILIYVSKL
260 270 280 290 300
EIFMGITFIV ISKEHKLIKF IENKLPSIAK LIKNYNNEKT LELNLRKKTK
310 320 330 340 350
DGIFTSLFAI HPISKKILPI WISNFFFTNN DIYQSIAAIP AYNKNELDFA
360 370 380 390 400
KKYNLPIRYV IKDYFEKIID FKKYNNLKEG ILFNSNIFNG LNLKNGYDRI
410 420 430 440 450
SKFLISNKIG KRSTHYKLRD WCISRQRYWG APIPVLITKE NKILVVSENE
460 470 480 490 500
LPVILPKAKN NNIIHSLNSY KDWIYVLNDN KLVKREVDTF DTFMESSWYL
510 520 530 540 550
HRYTCTKYTK DILDPNATNY WFPVDQYIGG IEHATMHLLY LRFYHKILRD
560 570 580 590 600
MNFVKCDEPV NRLLCQGMVL SDTFYYFSKS GNKIWTSPKN KNFERNKDNK
610 620 630 640 650
IINAIDSLGN KLTHIGMSKM SKSKNNGVDP QGIINKYGSD TLRLFIMFAA
660 670 680 690 700
PPELSLEWSD KGIIGANRFI KKLWKITYNY LNLKKNNNYV FYKKLKEQDN
710 720 730 740 750
ILLEKSFYVI NKVTKDIDKN QTFNTAIAEI MKLTNHLNSY INKNEYNNIS
760 770 780 790 800
IIKKVLMIII RLLYPFIPHV CFVLWNEINK NNDIDKTKWP KIKMPFIKNK
810 820 830 840 850
KKNIVVQING KLKTVISFDI SCNEFLIKKC VIENNKIKNL LNKKKIKNII
860
YVKNKIINIV LDDK
Length:864
Mass (Da):102,169
Last modified:March 1, 2003 - v1
Checksum:i62C8A9BD84031F3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000021 Genomic DNA. Translation: BAC24314.1.
RefSeqiNP_871171.1. NC_004344.2.

Genome annotation databases

EnsemblBacteriaiBAC24314; BAC24314; BAC24314.
KEGGiwbr:WGLp168.
PATRICi24024491. VBIWigGlo15804_0188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000021 Genomic DNA. Translation: BAC24314.1.
RefSeqiNP_871171.1. NC_004344.2.

3D structure databases

ProteinModelPortaliQ8D333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi36870.WGLp168.

Proteomic databases

PRIDEiQ8D333.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC24314; BAC24314; BAC24314.
KEGGiwbr:WGLp168.
PATRICi24024491. VBIWigGlo15804_0188.

Phylogenomic databases

eggNOGiCOG0495.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
    Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
    Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYL_WIGBR
AccessioniPrimary (citable) accession number: Q8D333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: April 1, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.