Q8D325 (LIPA_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase
EC=2.8.1.8

Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA

Gene names

Name:	lipA
Ordered Locus Names:	WIGBR1760

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia › Wigglesworthia glossinidia

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00206.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Lipoyl synthase HAMAP-Rule MF_00206		PRO_0000102381
Sites
	Metal binding	66	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	71	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	77	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	92	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	96	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	99	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D325 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1EDB67EE0C274D23	FASTA	316	36,200
	10 20 30 40 50 60 MKSISYLNTK EKNKKKLSIP SKTIFAKENE YGLKKPNWLK IKLPLNNKKI NKIKLIMRKN 70 80 90 100 110 120 NLHTVCEEAA CPNLAECFNR GTATFMILGS ICTRRCPFCN VSSGRPSLVD TKEPENLSKA 130 140 150 160 170 180 AIKMKLKHIV ITSVDRDDLK DGGSEHFSNC IRFIRKKNPD IKIEILVPDF RGCTELALNN 190 200 210 220 230 240 ISTYPPDIFN HNLESIPRLY SKVRPGANYK RSLELLEKFN LINPNIPTKS GLMLGLGETK 250 260 270 280 290 300 EEIIEVMKDL RKSYVSMITI GQYLRPTKNH LTVNRYVHPK EFRELNLIAF DLGFKHAMCG 310 PLVRSSYHAE NQINCY

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24322.1.
RefSeq	NP_871179.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D325.
ModBase	Search...
Protein-protein interaction databases
STRING	36870.WGLp176.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC24322; BAC24322; BAC24322.
GeneID	1256971.
KEGG	wbr:WGLp176.
PATRIC	24024507. VBIWigGlo15804_0196.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0320.
KO	K03644.
OMA	EEYVTPE.
ProtClustDB	PRK05481.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00206. Lipoyl_synth.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIPA_WIGBR

Accession

Primary (citable) accession number: Q8D325

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program