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Q8D2Y5 (DDL_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Synonyms:ddlA
Ordered Locus Names:WIGBR2170
OrganismWigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]
Taxonomic identifier36870 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177905

Regions

Domain143 – 344202ATP-grasp
Nucleotide binding172 – 22756ATP By similarity

Sites

Metal binding2981Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 1 By similarity
Metal binding3111Magnesium or manganese 2 By similarity
Metal binding3131Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D2Y5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 4EF5BEC332975F10

FASTA35039,675
        10         20         30         40         50         60 
MKKTVAVLFG GESKEHEISL QSSISIINAI DKKKYNIILI GVEKNGKIGI RSINNYILFK 

        70         80         90        100        110        120 
YNINYIKLAP AVSYLYIIPG KNNYQFYSLK NKKMLKIDVI FSILHGSNGE NGAFQGLFNT 

       130        140        150        160        170        180 
IYTPFVGSNV LSSSICMDKD ISKRILSTFG ISVVPSITLY YENYKKKINK IINNIKFPCC 

       190        200        210        220        230        240 
IKPSNQGSSF GVNVANDFIS LKESIDVAFL YSKKILIEPF IQGREIEVGV LGNRNVISSV 

       250        260        270        280        290        300 
CGEIKFKKIF YDYKEKYISK KTKIIIPAKI SNEISNKIKK IAKLAFISLE CSIMARVDFF 

       310        320        330        340        350 
LTKNKKIFLN EINTIPGFTK NSIYPKLWSK SGLDFKSLIN KLILLTIYKK 

« Hide

References

[1]"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000021 Genomic DNA. Translation: BAC24363.1.
RefSeqNP_871220.1. NC_004344.2.

3D structure databases

ProteinModelPortalQ8D2Y5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36870.WGLp217.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC24363; BAC24363; BAC24363.
GeneID1257012.
KEGGwbr:WGLp217.
PATRIC24024589. VBIWigGlo15804_0237.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
KOK01921.
OMAQIDVIFP.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_WIGBR
AccessionPrimary (citable) accession number: Q8D2Y5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways