Q8D2Y0 (PNP_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyribonucleotide nucleotidyltransferase
EC=2.7.7.8

Alternative name(s):
Polynucleotide phosphorylase
Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	WIGBR2220

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia › Wigglesworthia glossinidia

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity. HAMAP-Rule MF_01595
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01595.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: HAMAP polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	Polyribonucleotide nucleotidyltransferase HAMAP-Rule MF_01595		PRO_0000329934
Regions
	Domain	553 – 612	60	KH
	Domain	622 – 690	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q8D2Y0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9EC1E1941F7407DF	FASTA	705	79,404
	10 20 30 40 50 60 MLKATIQKFQ YGKNIVTIET GMIARQATSA VMVRMDDTSV FVTVVADKKE KLGQKFFPLT 70 80 90 100 110 120 VNYQERTYSI GRFPGGFFRR EGRPNENEVL VSRLIDRSVR PLFPKNFFND IQIIATVMSV 130 140 150 160 170 180 NPQVNPDIVS IIGASAALSL SGLPFKCPIG SSRIGYIDNK YILNPTTSEL IDSKLDMIVS 190 200 210 220 230 240 GSSNAVLMVE SSSNILQEKN ILEAILFGNE QNKIVIENID KLKKKFKKEN LEYDLKSLNN 250 260 270 280 290 300 NDIQFVIEDL FFDRIKDSYK IPEKKKRLEK IENIKNDILN NFINNNNTNE EEILYIIQNI 310 320 330 340 350 360 ERKIVRNRII SGKPRIDGRT EDMVRNLDIH LGILPRTHGS SLFTRGETQA LVTTTLGTER 370 380 390 400 410 420 DAQNIDDLVG DKIDRFLFHY NFPPYCVGEV GIISSPKRRE IGHGKLAKRG MLAVMPDIDK 430 440 450 460 470 480 FPYTIRIVSE ITESNGSSSM ASVCGASLSL MDAGVPISSS VAGIAMGLIK EKDKFVVLTD 490 500 510 520 530 540 ILGDEDYLGD MDFKVMGTRE GVTALQMDIK IEGITSEILQ VSLDKAKNAR INILNEMDKV 550 560 570 580 590 600 IKKPKNEISI FAPRIYKIKI NPEKIKDVIG KGGSVIRMLT EKTKSSIEIE DDGTVKVIST 610 620 630 640 650 660 DIKNAQCALK KIKDITHEIK INKIYVAKIT RISEFGIFAC LINNKEGLIH VSKIPYKKFS 670 680 690 700 DINNNFKIGQ IISVKVIEID RYGRIRLSFT GTDKNKNKKF FNKNN

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24368.1.
RefSeq	NP_871225.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D2Y0.
ModBase	Search...
Protein-protein interaction databases
STRING	36870.WGLp222.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC24368; BAC24368; BAC24368.
GeneID	1257017.
KEGG	wbr:WGLp222.
PATRIC	24024599. VBIWigGlo15804_0242.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1185.
KO	K00962.
OMA	YTMRVVS.
ProtClustDB	PRK11824.
Family and domain databases
Gene3D	1.10.10.400. 1 hit. 2.40.50.140. 1 hit. 3.30.230.70. 2 hits.
HAMAP	MF_01595. PNPase.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH_dom. IPR004088. KH_dom_type_1. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR027408. PNPase/RNase_PH_dom. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR020568. Ribosomal_S5_D2-typ_fold. IPR022967. RNA-binding_domain_S1. [Graphical view]
PANTHER	PTHR11252. PTHR11252. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF46915. 3_ExoRNase. 1 hit. SSF55666. 3_ExoRNase. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 2 hits.
TIGRFAMs	TIGR03591. polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_WIGBR

Accession

Primary (citable) accession number: Q8D2Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program