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Protein

3,4-dihydroxy-2-butanone 4-phosphate synthase

Gene

ribB

Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.UniRule annotation

Catalytic activityi

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 2 divalent metal cations per subunit. Magnesium or manganese.UniRule annotation

Pathwayi: riboflavin biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB)
This subpathway is part of the pathway riboflavin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate, the pathway riboflavin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Magnesium or manganese 1UniRule annotation1
Metal bindingi38Magnesium or manganese 2UniRule annotation1
Binding sitei42SubstrateUniRule annotation1
Sitei136Essential for catalytic activityUniRule annotation1
Metal bindingi153Magnesium or manganese 2UniRule annotation1
Binding sitei174SubstrateUniRule annotation1
Sitei174Essential for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processRiboflavin biosynthesis
LigandMagnesium, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00275; UER00399.

Names & Taxonomyi

Protein namesi
Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthaseUniRule annotation (EC:4.1.99.12UniRule annotation)
Short name:
DHBP synthaseUniRule annotation
Gene namesi
Name:ribBUniRule annotation
Ordered Locus Names:WIGBR2370
OrganismiWigglesworthia glossinidia brevipalpis
Taxonomic identifieri36870 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeWigglesworthia
Proteomesi
  • UP000000562 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001518191 – 2133,4-dihydroxy-2-butanone 4-phosphate synthaseAdd BLAST213

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi36870.WGLp237.

Structurei

3D structure databases

ProteinModelPortaliQ8D2W5.
SMRiQ8D2W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 38Substrate bindingUniRule annotation2
Regioni150 – 154Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the DHBP synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C66. Bacteria.
COG0108. LUCA.
KOiK02858.
OMAiVVCEMLD.
OrthoDBiPOG091H008U.

Family and domain databases

Gene3Di3.90.870.10. 1 hit.
HAMAPiMF_00180. RibB. 1 hit.
InterProiView protein in InterPro
IPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
PfamiView protein in Pfam
PF00926. DHBP_synthase. 1 hit.
SUPFAMiSSF55821. SSF55821. 1 hit.
TIGRFAMsiTIGR00506. ribB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8D2W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKKLLSEFG DPVQRVKNAL QSLKRGNGIL VLDDENRENE GDMIFAAENM
60 70 80 90 100
TVNQMALAIR FGSGIVCICL TKEKCKILNL PMMVKKNNSK YKTAFTVSIE
110 120 130 140 150
SAYGITTGVS AYDRIITIKT AIKDNAKPDD LNRPGHVFPL QAHPGGILSR
160 170 180 190 200
AGHTEAAVDL TKLAGLKPAG VICEVMNDDG SMARMPEIIN FSKKYNMPVL
210
IIKDIIEYYN FIN
Length:213
Mass (Da):23,510
Last modified:March 1, 2003 - v1
Checksum:i5FA5F3B5E3D6C23E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000021 Genomic DNA. Translation: BAC24383.1.
RefSeqiWP_011070041.1. NC_004344.2.

Genome annotation databases

EnsemblBacteriaiBAC24383; BAC24383; BAC24383.
KEGGiwbr:ribB.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiRIBB_WIGBR
AccessioniPrimary (citable) accession number: Q8D2W5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: March 1, 2003
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families