Q8D2W5 (RIBB_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase
Short name=DHBP synthase
EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	WIGBR2370

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia › Wigglesworthia glossinidia

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP-Rule MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_00180
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHBP synthase family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP-Rule MF_00180		PRO_0000151819
Regions
	Region	37 – 38	2	Substrate binding By similarity
	Region	150 – 154	5	Substrate binding By similarity
Sites
	Metal binding	38	1	Magnesium or manganese 1 By similarity
	Metal binding	38	1	Magnesium or manganese 2 By similarity
	Metal binding	153	1	Magnesium or manganese 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	174	1	Substrate By similarity
	Site	136	1	Essential for catalytic activity By similarity
	Site	174	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D2W5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5FA5F3B5E3D6C23E	FASTA	213	23,510
	10 20 30 40 50 60 MNKKLLSEFG DPVQRVKNAL QSLKRGNGIL VLDDENRENE GDMIFAAENM TVNQMALAIR 70 80 90 100 110 120 FGSGIVCICL TKEKCKILNL PMMVKKNNSK YKTAFTVSIE SAYGITTGVS AYDRIITIKT 130 140 150 160 170 180 AIKDNAKPDD LNRPGHVFPL QAHPGGILSR AGHTEAAVDL TKLAGLKPAG VICEVMNDDG 190 200 210 SMARMPEIIN FSKKYNMPVL IIKDIIEYYN FIN

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24383.1.
RefSeq	NP_871240.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D2W5.
SMR	Q8D2W5. Positions 1-208.
ModBase	Search...
Protein-protein interaction databases
STRING	36870.WGLp237.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC24383; BAC24383; BAC24383.
GeneID	1257036.
KEGG	wbr:WGLp237.
PATRIC	24024639. VBIWigGlo15804_0258.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0108.
KO	K02858.
OMA	QMAKLIR.
Enzyme and pathway databases
UniPathway	UPA00275; UER00399.
Family and domain databases
Gene3D	3.90.870.10. 1 hit.
HAMAP	MF_00180. RibB.
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_WIGBR

Accession

Primary (citable) accession number: Q8D2W5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program