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Q8D2W5 (RIBB_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase

Short name=DHBP synthase
EC=4.1.99.12
Gene names
Name:ribB
Ordered Locus Names:WIGBR2370
OrganismWigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]
Taxonomic identifier36870 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia

Protein attributes

Sequence length213 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP-Rule MF_00180

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_00180

Cofactor

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity.

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_00180

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00180

Sequence similarities

Belongs to the DHBP synthase family.

Ontologies

Keywords
   Biological processRiboflavin biosynthesis
   LigandMagnesium
Manganese
Metal-binding
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processriboflavin biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_function3,4-dihydroxy-2-butanone-4-phosphate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2132133,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP-Rule MF_00180
PRO_0000151819

Regions

Region37 – 382Substrate binding By similarity
Region150 – 1545Substrate binding By similarity

Sites

Metal binding381Magnesium or manganese 1 By similarity
Metal binding381Magnesium or manganese 2 By similarity
Metal binding1531Magnesium or manganese 2 By similarity
Binding site421Substrate By similarity
Binding site1741Substrate By similarity
Site1361Essential for catalytic activity By similarity
Site1741Essential for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D2W5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 5FA5F3B5E3D6C23E

FASTA21323,510
        10         20         30         40         50         60 
MNKKLLSEFG DPVQRVKNAL QSLKRGNGIL VLDDENRENE GDMIFAAENM TVNQMALAIR 

        70         80         90        100        110        120 
FGSGIVCICL TKEKCKILNL PMMVKKNNSK YKTAFTVSIE SAYGITTGVS AYDRIITIKT 

       130        140        150        160        170        180 
AIKDNAKPDD LNRPGHVFPL QAHPGGILSR AGHTEAAVDL TKLAGLKPAG VICEVMNDDG 

       190        200        210 
SMARMPEIIN FSKKYNMPVL IIKDIIEYYN FIN 

« Hide

References

[1]"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000021 Genomic DNA. Translation: BAC24383.1.
RefSeqNP_871240.1. NC_004344.2.

3D structure databases

ProteinModelPortalQ8D2W5.
SMRQ8D2W5. Positions 1-208.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36870.WGLp237.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC24383; BAC24383; BAC24383.
GeneID1257036.
KEGGwbr:WGLp237.
PATRIC24024639. VBIWigGlo15804_0258.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0108.
KOK02858.
OMAQMAKLIR.
OrthoDBEOG679TK8.

Enzyme and pathway databases

UniPathwayUPA00275; UER00399.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
HAMAPMF_00180. RibB.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
[Graphical view]
SUPFAMSSF55821. SSF55821. 1 hit.
TIGRFAMsTIGR00506. ribB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRIBB_WIGBR
AccessionPrimary (citable) accession number: Q8D2W5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways