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Q8D2R0

- SYI_WIGBR

UniProt

Q8D2R0 - SYI_WIGBR

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
    Binding sitei607 – 6071ATPUniRule annotation
    Metal bindingi903 – 9031ZincUniRule annotation
    Metal bindingi906 – 9061ZincUniRule annotation
    Metal bindingi923 – 9231ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:WIGBR2940
    OrganismiWigglesworthia glossinidia brevipalpis
    Taxonomic identifieri36870 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia
    ProteomesiUP000000562: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 940940Isoleucine--tRNA ligasePRO_0000098502Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi36870.WGLp294.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8D2R0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi604 – 6085"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8D2R0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNDYKNTLN LPKTKFPMKA NLLISELKIL ECWKKNNLYS IIRENKKGKK    50
    TFFLHDGPPY ANGDIHIGHA VNKILKDIII KFKSLFGFDA PYMPGWDCHG 100
    LPIELQVEKK INQNINKINH KIFREKCRNY ALDQVNKQKK EFIRLGVIGD 150
    WNNPYLTMDF HTEANILRTF KNLIKKKYLY RGIKPINWCI DCKSSLSDSE 200
    IDYNIKESNS IDVSFSACDN NKILKIFNSE KKFDKIKAII WTTTPWTIIA 250
    NRAISVNPNF IYLLVKCNKE IFIIAESLLD KTLARLNIKK SKILGSVYGK 300
    KLKFLYFKHP LNKIHVPMIL NEYVDFKSGS GIVHVAPNYG EEDYIIGKKY 350
    KLNMYDPIDS NGNYLPGTFP GLDGLNIFKS EEVVLNLLKN NHSLYSKKII 400
    NHSYPHCWRH KSPTFFRATN QWFFNIDNNN LRNKTISEIK KILWIPKWGM 450
    NQIIDLLSNR PDWCISRQRV WGVPIAVFIN KKNKEIHPNT VELIEKVSKK 500
    IEKKGVQAWW DINKSEILDK DSDKYKKVLD TLDVWFDSGS TYYSILIKKW 550
    NSLNKNKVDL YLEGSDQYRG WFMSSIILSI AITGGIPCKK ILAHGFVVDS 600
    NGKKMSKSIG NVISPKDIIN EFGADILRLW VASSDYKSDI SISKEILLRT 650
    VDIYRRIRNT SRFLLSNLND FNPEFNSINL ENMLSIDQWA IIKTNNRQKK 700
    IKNAYEEYNF HKVVSNIVDF CSLDMGSFYL DIIKDRQYTN YIDSLARRSC 750
    QTAILYIIEC LVRWIMPILS FTSHEIWKYI PGKREKYVFL SEWVKEIPCE 800
    NINSYISKSL WKILIKIKNE INKIIEIKKL EDKIKSSLEL KITLYAESFI 850
    FEELILLKKE LNFAFLVSYV SIKKYENSKE NAFKSKSIKN LKILAEKFDG 900
    KKCLRCWNYT NDIVNDVKYN KICNRCINNI NGVEEKRKYF 940
    Length:940
    Mass (Da):110,118
    Last modified:March 1, 2003 - v1
    Checksum:iF7443E32F0FC4BFE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000021 Genomic DNA. Translation: BAC24440.1.
    RefSeqiNP_871297.1. NC_004344.2.

    Genome annotation databases

    EnsemblBacteriaiBAC24440; BAC24440; BAC24440.
    GeneIDi1257094.
    KEGGiwbr:WGLp294.
    PATRICi24024772. VBIWigGlo15804_0323.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000021 Genomic DNA. Translation: BAC24440.1 .
    RefSeqi NP_871297.1. NC_004344.2.

    3D structure databases

    ProteinModelPortali Q8D2R0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 36870.WGLp294.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC24440 ; BAC24440 ; BAC24440 .
    GeneIDi 1257094.
    KEGGi wbr:WGLp294.
    PATRICi 24024772. VBIWigGlo15804_0323.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
      Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
      Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_WIGBR
    AccessioniPrimary (citable) accession number: Q8D2R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3