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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei563Aminoacyl-adenylateUniRule annotation1
Binding sitei607ATPUniRule annotation1
Metal bindingi903ZincUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi923ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:WIGBR2940
OrganismiWigglesworthia glossinidia brevipalpis
Taxonomic identifieri36870 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeWigglesworthia
Proteomesi
  • UP000000562 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985021 – 940Isoleucine--tRNA ligaseAdd BLAST940

Proteomic databases

PRIDEiQ8D2R0

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi36870.WGLp294

Structurei

3D structure databases

ProteinModelPortaliQ8D2R0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi604 – 608"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8D2R0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNDYKNTLN LPKTKFPMKA NLLISELKIL ECWKKNNLYS IIRENKKGKK
60 70 80 90 100
TFFLHDGPPY ANGDIHIGHA VNKILKDIII KFKSLFGFDA PYMPGWDCHG
110 120 130 140 150
LPIELQVEKK INQNINKINH KIFREKCRNY ALDQVNKQKK EFIRLGVIGD
160 170 180 190 200
WNNPYLTMDF HTEANILRTF KNLIKKKYLY RGIKPINWCI DCKSSLSDSE
210 220 230 240 250
IDYNIKESNS IDVSFSACDN NKILKIFNSE KKFDKIKAII WTTTPWTIIA
260 270 280 290 300
NRAISVNPNF IYLLVKCNKE IFIIAESLLD KTLARLNIKK SKILGSVYGK
310 320 330 340 350
KLKFLYFKHP LNKIHVPMIL NEYVDFKSGS GIVHVAPNYG EEDYIIGKKY
360 370 380 390 400
KLNMYDPIDS NGNYLPGTFP GLDGLNIFKS EEVVLNLLKN NHSLYSKKII
410 420 430 440 450
NHSYPHCWRH KSPTFFRATN QWFFNIDNNN LRNKTISEIK KILWIPKWGM
460 470 480 490 500
NQIIDLLSNR PDWCISRQRV WGVPIAVFIN KKNKEIHPNT VELIEKVSKK
510 520 530 540 550
IEKKGVQAWW DINKSEILDK DSDKYKKVLD TLDVWFDSGS TYYSILIKKW
560 570 580 590 600
NSLNKNKVDL YLEGSDQYRG WFMSSIILSI AITGGIPCKK ILAHGFVVDS
610 620 630 640 650
NGKKMSKSIG NVISPKDIIN EFGADILRLW VASSDYKSDI SISKEILLRT
660 670 680 690 700
VDIYRRIRNT SRFLLSNLND FNPEFNSINL ENMLSIDQWA IIKTNNRQKK
710 720 730 740 750
IKNAYEEYNF HKVVSNIVDF CSLDMGSFYL DIIKDRQYTN YIDSLARRSC
760 770 780 790 800
QTAILYIIEC LVRWIMPILS FTSHEIWKYI PGKREKYVFL SEWVKEIPCE
810 820 830 840 850
NINSYISKSL WKILIKIKNE INKIIEIKKL EDKIKSSLEL KITLYAESFI
860 870 880 890 900
FEELILLKKE LNFAFLVSYV SIKKYENSKE NAFKSKSIKN LKILAEKFDG
910 920 930 940
KKCLRCWNYT NDIVNDVKYN KICNRCINNI NGVEEKRKYF
Length:940
Mass (Da):110,118
Last modified:March 1, 2003 - v1
Checksum:iF7443E32F0FC4BFE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000021 Genomic DNA Translation: BAC24440.1

Genome annotation databases

EnsemblBacteriaiBAC24440; BAC24440; BAC24440
KEGGiwbr:ileS

Similar proteinsi

Entry informationi

Entry nameiSYI_WIGBR
AccessioniPrimary (citable) accession number: Q8D2R0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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