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Q8D2R0

- SYI_WIGBR

UniProt

Q8D2R0 - SYI_WIGBR

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei563 – 5631Aminoacyl-adenylateUniRule annotation
Binding sitei607 – 6071ATPUniRule annotation
Metal bindingi903 – 9031ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi923 – 9231ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:WIGBR2940
OrganismiWigglesworthia glossinidia brevipalpis
Taxonomic identifieri36870 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia
ProteomesiUP000000562: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 940940Isoleucine--tRNA ligasePRO_0000098502Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi36870.WGLp294.

Structurei

3D structure databases

ProteinModelPortaliQ8D2R0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi604 – 6085"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8D2R0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMNDYKNTLN LPKTKFPMKA NLLISELKIL ECWKKNNLYS IIRENKKGKK
60 70 80 90 100
TFFLHDGPPY ANGDIHIGHA VNKILKDIII KFKSLFGFDA PYMPGWDCHG
110 120 130 140 150
LPIELQVEKK INQNINKINH KIFREKCRNY ALDQVNKQKK EFIRLGVIGD
160 170 180 190 200
WNNPYLTMDF HTEANILRTF KNLIKKKYLY RGIKPINWCI DCKSSLSDSE
210 220 230 240 250
IDYNIKESNS IDVSFSACDN NKILKIFNSE KKFDKIKAII WTTTPWTIIA
260 270 280 290 300
NRAISVNPNF IYLLVKCNKE IFIIAESLLD KTLARLNIKK SKILGSVYGK
310 320 330 340 350
KLKFLYFKHP LNKIHVPMIL NEYVDFKSGS GIVHVAPNYG EEDYIIGKKY
360 370 380 390 400
KLNMYDPIDS NGNYLPGTFP GLDGLNIFKS EEVVLNLLKN NHSLYSKKII
410 420 430 440 450
NHSYPHCWRH KSPTFFRATN QWFFNIDNNN LRNKTISEIK KILWIPKWGM
460 470 480 490 500
NQIIDLLSNR PDWCISRQRV WGVPIAVFIN KKNKEIHPNT VELIEKVSKK
510 520 530 540 550
IEKKGVQAWW DINKSEILDK DSDKYKKVLD TLDVWFDSGS TYYSILIKKW
560 570 580 590 600
NSLNKNKVDL YLEGSDQYRG WFMSSIILSI AITGGIPCKK ILAHGFVVDS
610 620 630 640 650
NGKKMSKSIG NVISPKDIIN EFGADILRLW VASSDYKSDI SISKEILLRT
660 670 680 690 700
VDIYRRIRNT SRFLLSNLND FNPEFNSINL ENMLSIDQWA IIKTNNRQKK
710 720 730 740 750
IKNAYEEYNF HKVVSNIVDF CSLDMGSFYL DIIKDRQYTN YIDSLARRSC
760 770 780 790 800
QTAILYIIEC LVRWIMPILS FTSHEIWKYI PGKREKYVFL SEWVKEIPCE
810 820 830 840 850
NINSYISKSL WKILIKIKNE INKIIEIKKL EDKIKSSLEL KITLYAESFI
860 870 880 890 900
FEELILLKKE LNFAFLVSYV SIKKYENSKE NAFKSKSIKN LKILAEKFDG
910 920 930 940
KKCLRCWNYT NDIVNDVKYN KICNRCINNI NGVEEKRKYF
Length:940
Mass (Da):110,118
Last modified:March 1, 2003 - v1
Checksum:iF7443E32F0FC4BFE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000021 Genomic DNA. Translation: BAC24440.1.
RefSeqiNP_871297.1. NC_004344.2.

Genome annotation databases

EnsemblBacteriaiBAC24440; BAC24440; BAC24440.
GeneIDi1257094.
KEGGiwbr:WGLp294.
PATRICi24024772. VBIWigGlo15804_0323.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000021 Genomic DNA. Translation: BAC24440.1 .
RefSeqi NP_871297.1. NC_004344.2.

3D structure databases

ProteinModelPortali Q8D2R0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36870.WGLp294.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC24440 ; BAC24440 ; BAC24440 .
GeneIDi 1257094.
KEGGi wbr:WGLp294.
PATRICi 24024772. VBIWigGlo15804_0323.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
    Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
    Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_WIGBR
AccessioniPrimary (citable) accession number: Q8D2R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2003
Last modified: October 29, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3