Reviewed, UniProtKB/Swiss-Prot Q8D2P7 (SURE_WIGBR)

Names and origin

Protein names

Recommended name:
    Multifunctional protein surE
Including the following 2 domains:
    1- Recommended name:
            5'/3'-nucleotidase
              EC=3.1.3.5
              EC=3.1.3.6
        Alternative name(s):
            Nucleoside monophosphate phosphohydrolase
    2- Recommended name:
            Exopolyphosphatase
              EC=3.6.1.11

Gene names

Name:	surE
Ordered Locus Names:	WIGBR3070

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nucleotidase with a broad substrate specificity as it can dephosphorylate various ribo- and deoxyribonucleoside 5'-monophosphates and ribonucleoside 3'-monophosphates with highest affinity to 3'-AMP. Also hydrolyzes polyphosphate (exopolyphosphatase activity) with the preference for short-chain-length substrates (P20-25). Might be involved in the regulation of dNTP and NTP pools, and in the turnover of 3'-mononucleotides produced by numerous intracellular RNases (T1, T2, and F) during the degradation of various RNAs By similarity.
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060 A 3'-ribonucleotide + H2O = a ribonucleoside + phosphate. HAMAP MF_00060 (Polyphosphate)(n) + H2O = (polyphosphate)(n-1) + phosphate. HAMAP MF_00060
Cofactor	Binds 1 divalent metal cation per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the surE nucleotidase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-nucleotidase activity Inferred from electronic annotation. Source: EC 5'-nucleotidase activity Inferred from electronic annotation. Source: HAMAP exopolyphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: HAMAP nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Multifunctional protein surE HAMAP MF_00060		PRO_0000111853
Sites
	Metal binding	8	1	Divalent metal cation By similarity
	Metal binding	9	1	Divalent metal cation By similarity
	Metal binding	39	1	Divalent metal cation By similarity
	Metal binding	92	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D2P7-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: D503F7A056024EA9	FASTA	250	27,998
	10 20 30 40 50 60 MNILLSNDDG IYSPGIQKLS KKLKKFLNVQ VIAPSCDKSG SSSSLTINNP LKVHKFSNGD 70 80 90 100 110 120 ITVYSGTPID CVYLGINFFM KPKPDFVVSG INLGANLGDD VFYSGTVGAA MEGRYLKYSS 130 140 150 160 170 180 LAISLDGNKH LDVAVEIVYK FLKFLLNNPF RKKYILNINI PDSPLKYIKG FKITKCGRKN 190 200 210 220 230 240 FKNTVIKSKD SENKNIFWIG PKTNCYNESI GTDFHAIKNN YISVTPLLSN LTNNKEINSI 250 SNWFENFYKS

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed: 12219091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000021 Genomic DNA. Translation: BAC24453.1.
RefSeq	NP_871310.1.
3D structure databases
HSSP	HSSP built from PDB template 1J9L based on UniProtKB P96112.
ModBase	Search...
Genome annotation databases
GeneID	1257110.
GenomeReviews	Gene locus WIGBR3070 in contig BA000021_GR.
KEGG	wbr:WGLp307.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8D2P7.
OMA	Q8D2P7. SINIPIK.
Family and domain databases
HAMAP	MF_00060. [Tree]
InterPro	IPR002828. SurE-like_Pase/nucleotidase. [Graphical view]
Gene3D	G3DSA:3.40.1210.10. SurE-like_Pase/nucleotidase. 1 hit.
Pfam	PF01975. SurE. 1 hit. [Graphical view]
ProDom	PD005378. SurE. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00087. surE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SURE_WIGBR

Accession

Primary (citable) accession number: Q8D2P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)