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Q8D2N4 (TYSY_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:WIGBR3200
OrganismWigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]
Taxonomic identifier36870 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia

Protein attributes

Sequence length264 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 264264Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141044

Sites

Active site1461 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D2N4 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7D679A07D5EDBEA6

FASTA26430,804
        10         20         30         40         50         60 
MKEYLDLLNL ILKNGYPKID RTKTGTLSMF GYQIRINLNE GFPLLTTKYC HFKSIVYELL 

        70         80         90        100        110        120 
WFLRGDTNIS FLKKNNISIW NKWADKNGNL GPIYGKQWRA WEDKKNNTID QIEIALNKLK 

       130        140        150        160        170        180 
KEPSSRRILV SSWNVGELDL MSIPPCHVLF QLYVINNKLS CQVYQRSCDI FLGLPFNIGS 

       190        200        210        220        230        240 
YALLTHIFAN QCDLLVEDLI WTGGDIHLYK NHLNQAKLQL TRSPLPLPKI FIKKKPKNLF 

       250        260 
NYAFNDFLLI DYNHHPKIKA PISI 

« Hide

References

[1]"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000021 Genomic DNA. Translation: BAC24466.1.
RefSeqNP_871323.1. NC_004344.2.

3D structure databases

ProteinModelPortalQ8D2N4.
SMRQ8D2N4. Positions 1-264.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36870.WGLp320.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC24466; BAC24466; BAC24466.
GeneID1257126.
KEGGwbr:WGLp320.
PATRIC24024838. VBIWigGlo15804_0350.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
KOK00560.
OMADVHLYSN.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_WIGBR
AccessionPrimary (citable) accession number: Q8D2N4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways