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Q8D2A1

- BIOB_WIGBR

UniProt

Q8D2A1 - BIOB_WIGBR

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Protein
Biotin synthase
Gene
bioB, WIGBR4530
Organism
Wigglesworthia glossinidia brevipalpis
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi57 – 571Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi60 – 601Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi97 – 971Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi128 – 1281Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi188 – 1881Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi260 – 2601Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:WIGBR4530
OrganismiWigglesworthia glossinidia brevipalpis
Taxonomic identifieri36870 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia
ProteomesiUP000000562: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 341341Biotin synthaseUniRule annotation
PRO_0000381704Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi36870.WGLp453.

Structurei

3D structure databases

ProteinModelPortaliQ8D2A1.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8D2A1-1 [UniParc]FASTAAdd to Basket

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MKNKSIWNLK NTKKLFYKPF FDLMFKAQKI HRKNFSSNKI QISTLLSIKT    50
GLCPEDCKYC SQSSRYKTGL VSEKLIKIEK VINAAKKAKK SGVTRFCMGA 100
AWKNPKDKDI PLLEEMIKSV KKLGLETCMT LGMLNDYQAN RLHNAGLDYY 150
NHNLDTSKEF YKKIITTRKY EDRISTIQNV RNAGIKVCSG GILGLGESDK 200
DRIKLLIQLA NLPKPPESVP INMLVRIKGT PMEKNKDVDA FDFIRIIALA 250
RIMMPKSRIR LSAGRENMNE QTQALCFMAG VNSIFYGCKL LTTTNSSSKR 300
DALLFKKLNM KMEKIKNKEK YFNKKQNSDL YENKNYYNAV C 341
Length:341
Mass (Da):39,157
Last modified:July 28, 2009 - v2
Checksum:i542B929B6152ECDB
GO

Sequence cautioni

The sequence BAC24599.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000021 Genomic DNA. Translation: BAC24599.1. Different initiation.
RefSeqiNP_871456.1. NC_004344.2.

Genome annotation databases

EnsemblBacteriaiBAC24599; BAC24599; BAC24599.
GeneIDi1257263.
KEGGiwbr:WGLp453.
PATRICi24025124. VBIWigGlo15804_0489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000021 Genomic DNA. Translation: BAC24599.1 . Different initiation.
RefSeqi NP_871456.1. NC_004344.2.

3D structure databases

ProteinModelPortali Q8D2A1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 36870.WGLp453.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC24599 ; BAC24599 ; BAC24599 .
GeneIDi 1257263.
KEGGi wbr:WGLp453.
PATRICi 24025124. VBIWigGlo15804_0489.

Phylogenomic databases

eggNOGi COG0502.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
    Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
    Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiBIOB_WIGBR
AccessioniPrimary (citable) accession number: Q8D2A1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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