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Q8D245 (PUR2_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:WIGBR5100
OrganismWigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]
Taxonomic identifier36870 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeWigglesworthia

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151501

Regions

Domain109 – 316208ATP-grasp
Nucleotide binding135 – 19662ATP By similarity

Sites

Metal binding2861Magnesium or manganese By similarity
Metal binding2881Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8D245 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 216E313BDE34FC53

FASTA43448,909
        10         20         30         40         50         60 
MNILVVGNGG REHAISWKIA QSIFSKKIYV APGNAGTEID YKLKNIDISS NDINKLVKFA 

        70         80         90        100        110        120 
KEKKIDLTIV GPEEPLSNGI SDVFSYYKLP IFGPKKFSAK LESSKIFAKN FLNKYNIPTA 

       130        140        150        160        170        180 
RHKEFSDQKL AIKYLNRVKF PIVIKVDGLA FGKGVKIIKN IKESEKFILD VLVKKKFGMS 

       190        200        210        220        230        240 
GSKILIEDML HGEEISFTAI INNNGFLPMA ISQDHKKSGD KDTGLNTGGM GAYSPVSFVT 

       250        260        270        280        290        300 
SSIYNNIIKD ILLPTLYGMK KENLIYTGIL YIGIMIDYNN NVNVIEFNCR FGDPEAQSIL 

       310        320        330        340        350        360 
FRMKSDFLLH ILSAMYGNLD SQIIELDNRY SLGIVLATKS YPKKYPIGLN ILGNFENELK 

       370        380        390        400        410        420 
DTKIFHSSTK NINKNLTTNG GRVLCITSLG KSIEDAQKKS KIVANSINWQ GKFFRNDIGN 

       430 
REIFYLKKYK NYKF 

« Hide

References

[1]"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia."
Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S.
Nat. Genet. 32:402-407(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000021 Genomic DNA. Translation: BAC24656.1.
RefSeqNP_871513.1. NC_004344.2.

3D structure databases

ProteinModelPortalQ8D245.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING36870.WGLp510.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC24656; BAC24656; BAC24656.
GeneID1257321.
KEGGwbr:WGLp510.
PATRIC24025248. VBIWigGlo15804_0550.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_WIGBR
AccessionPrimary (citable) accession number: Q8D245
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways