Q8D223 (ISPD_WIGBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	WIGBR5320

Organism

Wigglesworthia glossinidia brevipalpis [Complete proteome] [HAMAP]

Taxonomic identifier

36870 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Wigglesworthia

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Subunit structure	Homodimer By similarity. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075648
Sites
	Site	19	1	Transition state stabilizer By similarity
	Site	26	1	Transition state stabilizer By similarity
	Site	154	1	Positions MEP for the nucleophilic attack By similarity
	Site	210	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8D223 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9CA1F49717BB9FD1	FASTA	229	26,240
	10 20 30 40 50 60 MINHPEIIVI FPAAGIGKRM GYKYPKQYIK IKNKTILEHS ISLFIDKIYV KKILIAINKK 70 80 90 100 110 120 DYWFNKLSIL KNKKINIVIG GKSRTESVIS ALKFVSKVDW VLVHDAVRPC LHKNDLNKLL 130 140 150 160 170 180 KVINISPFGA ILAAPIYDTV KKSYGNFISH TIKRNKLWRA LTPQLFNLKI LINCLKIITS 190 200 210 220 KGEIITDESS ALEKCGYKLN LVHGRSDNIK ITYPEDLNFA NFFINNIKE

References

[1]	"Genome sequence of the endocellular obligate symbiont of tsetse flies, Wigglesworthia glossinidia." Akman L., Yamashita A., Watanabe H., Oshima K., Shiba T., Hattori M., Aksoy S. Nat. Genet. 32:402-407(2002) [PubMed: 12219091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000021 Genomic DNA. Translation: BAC24678.1.
RefSeq	NP_871535.1. NC_004344.2.
3D structure databases
ProteinModelPortal	Q8D223.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8D223.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1257348.
GenomeReviews	Gene locus WIGBR5320 in contig BA000021_GR.
KEGG	wbr:WGLp532.
PATRIC	24025304. VBIWigGlo15804_0573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1211.
HOGENOM	HBG672839.
OMA	SKVIVVC.
PhylomeDB	Q8D223.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_WIGBR

Accession

Primary (citable) accession number: Q8D223

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program