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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Oceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei307NucleophileUniRule annotation1
Active sitei361Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
Alpha-(1->4)-glucan branching enzymeUniRule annotation
Glycogen branching enzymeUniRule annotation
Short name:
BEUniRule annotation
Gene namesi
Name:glgBUniRule annotation
Ordered Locus Names:OB0406
OrganismiOceanobacillus iheyensis (strain DSM 14371 / CIP 107618 / JCM 11309 / KCTC 3954 / HTE831)
Taxonomic identifieri221109 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeOceanobacillus
Proteomesi
  • UP000000822 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001887221 – 6371,4-alpha-glucan branching enzyme GlgBAdd BLAST637

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi221109.OB0406.

Structurei

3D structure databases

ProteinModelPortaliQ8CZE8.
SMRiQ8CZE8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000283037.
KOiK00700.
OMAiEVVHGKS.
OrthoDBiPOG091H08D1.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
HAMAPiMF_00685. GlgB. 1 hit.
InterProiView protein in InterPro
IPR006048. A-amylase/branching_C.
IPR006407. GlgB.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
PfamiView protein in Pfam
PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
PIRSFiPIRSF000463. GlgB. 1 hit.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CZE8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYHISAEDIY LFHQGTNFES HQFLGCHEIN WKGKRGYRFA VWAPNALKVC
60 70 80 90 100
VVGDFNNWEE NSHPLEKFTD EGLWGGFIAD IPPATSYKYH ICSSEDTSIL
110 120 130 140 150
KADPFATQAE RRPKTASVIP AANGYQWSDD QWIEQRNTYD PYSSPISIYE
160 170 180 190 200
VHLGTWKKTL KKQFLSYREL ATQLIPYVKS LGYTHIELLP INEHPFDRSW
210 220 230 240 250
GYQITGYFAV TSRYGNPSDF KYFIDQCHQH QIGVILDWVP GHFCKDDFGL
260 270 280 290 300
RQFDGAPLYE YRDPKKSEKK SWGTLAFDYG RPEVQSFLIS NAIYWLKEFH
310 320 330 340 350
IDGLRVDAVA SMLYLNFDRY DEEEKIYNTY GGEENLEAFA FLRKLNKVVF
360 370 380 390 400
SYIPGALMMA EDSSDLPLVT APVNKGGLGF NYKWNMGWMN DLLSFMEKES
410 420 430 440 450
IHRKWHHNRL TFSFMYTYSE NYLLPLSHDE VVHGKKSLLD KMPGDQWQQF
460 470 480 490 500
ANLRLLYGYM YTHPGKKLVF MGGELAQYAE WKDTEELDWH LLEYPLHKGI
510 520 530 540 550
YHYIKNLNEL YQQHPELYEL DHLSEGFEWI DPHNIDQSVI AFRRKANKPN
560 570 580 590 600
QELIIICNFT PQVHFDYKIG VPESGRYKEI FNSDSVRFGG SGQINEGEHF
610 620 630
SFPEKWHGLS QHIKIKVPPL AISVFQIEVE RERLPRE
Length:637
Mass (Da):74,651
Last modified:March 1, 2003 - v1
Checksum:i2A3165A2556A269D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000028 Genomic DNA. Translation: BAC12362.1.
RefSeqiWP_011064812.1. NC_004193.1.

Genome annotation databases

EnsemblBacteriaiBAC12362; BAC12362; BAC12362.
KEGGioih:OB0406.

Similar proteinsi

Entry informationi

Entry nameiGLGB_OCEIH
AccessioniPrimary (citable) accession number: Q8CZE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2003
Last modified: September 27, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families