Q8CYC9 (PFBA_STRR6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Plasmin and fibronectin-binding protein A

Gene names

Name:	pfbA
Ordered Locus Names:	spr1652

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Reference proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Streptococcus › Streptococcus pneumoniae

Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a fibronectin-dependent adhesin and invasin. Binds host (in this case human) fibronectin, plasmin, plasminogen, and human serum albumin. Where the bacteria adhere to human cells there is major recruitment of microvilli which seem to fuse to cover the streptococcal chains. Antibodies to this protein reduce bacterial growth in human blood.
Subcellular location	Secreted › cell wall; Peptidoglycan-anchor Probable Ref.2.
Disruption phenotype	Cells lacking this gene adhere to and invade human cell lines half as well as wild-type bacteria. They grow less well in human blood, perhaps because they are more resistant to phagocytosis. Ref.2
Biotechnological use	Has potential use as a vaccine component. Ref.2
Sequence similarities	Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Cell wall Secreted
Domain	Coiled coil Repeat Signal
PTM	Peptidoglycan-anchor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 45	45	Potential
	Chain	46 – 688	643	Plasmin and fibronectin-binding protein A		PRO_0000380122
	Propeptide	689 – 719	31	Removed by sortase Potential		PRO_0000380123
Regions
	Repeat	287 – 310	24	PbH1 1
	Repeat	362 – 384	23	PbH1 2
	Repeat	397 – 419	23	PbH1 3
	Repeat	497 – 523	27	PbH1 4
	Repeat	525 – 546	22	PbH1 5
	Coiled coil	601 – 658	58	Potential
	Motif	685 – 689	5	LPXTG sorting signal Potential
Amino acid modifications
	Modified residue	688	1	Pentaglycyl murein peptidoglycan amidated threonine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8CYC9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 5A4E3047262CBFBD	FASTA	719	79,016
	10 20 30 40 50 60 MLKIVKKLEV LMKYFVPNEV FSIRKLKVGT CSVLLAISIL GSQGILSDEV VTSSSPMATK 70 80 90 100 110 120 ESSNAITNDL DNSPTVNQNR SAEMIASNST TNGLDNSLSV NSISSNGTIR SNSQLDNRTV 130 140 150 160 170 180 ESTVTSTNEN KSYKEDVISD RIIKKEFEDT ALSVKDYGAV GDGIHDDRQA IQDAIDAAAQ 190 200 210 220 230 240 GLGGGNVYFP EGTYLVKEIV FLKSHTHLEL NEKATILNGI NIKNHPSIVF MTGLFTDDGA 250 260 270 280 290 300 QVEWGPTEDI SYSGGTIDMN GALNEEGTKA KNLPLINSSG AFAIGNSNNV TIKNVTFKDS 310 320 330 340 350 360 YQGHAIQIAG SKNVLVDNSR FLGQALPKTM KDGQIISKES IQIEPLTRKG FPYALNDDGK 370 380 390 400 410 420 KSENVTIQNS YFGKSDKSGE LVTAIGTHYQ TLSTQNPSNI KILNNHFDNM MYAGVRFTGF 430 440 450 460 470 480 TDVLIKGNRF DKKVKGESVH YRESGAALVN AYSYKNTKDL LDLNKQVVIA ENIFNIADPK 490 500 510 520 530 540 TKAIRVAKDS AEYLGKVSDI TVTKNVINNN SKETEQPNIE LLRVSDNLVV SENSIFGGKE 550 560 570 580 590 600 GIVIEDSKGK ITVLNNQFYN LSGKYISFIK SNANGKEPVI RDSDGNFNIV TENGLYKIVT 610 620 630 640 650 660 NNLSDKNEKE KNKEEKQSNS NNVIDSNQKN GEFNSSKDNR QMNDKIDNKQ DNKTEEVNYK 670 680 690 700 710 IVGDGRETEN HINKSKEIVD VKQKLPKTGS NKIMELFLTV TGIGLLLTLK GLKYYGKDK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-255 / R6.
[2]	"PfbA, a novel plasmin- and fibronectin-binding protein of Streptococcus pneumoniae, contributes to fibronectin-dependent adhesion and antiphagocytosis." Yamaguchi M., Terao Y., Mori Y., Hamada S., Kawabata S. J. Biol. Chem. 283:36272-36279(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, BINDING TO HOST EXTRACELLULAR MATRIX COMPONENTS, PROBABLE INVOLVEMENT IN VIRULENCE, BIOTECHNOLOGY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007317 Genomic DNA. Translation: AAL00455.1.
PIR	B98078.
RefSeq	NP_359244.1. NC_003098.1.
3D structure databases
ProteinModelPortal	Q8CYC9.
ModBase	Search...
Protein-protein interaction databases
STRING	171101.spr1652.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAL00455; AAL00455; spr1652.
GeneID	933108.
KEGG	spr:spr1652.
PATRIC	19703332. VBIStrPne107296_1781.
Phylogenomic databases
eggNOG	COG5434.
HOGENOM	HOG000285104.
KO	K13925.
OMA	TVESTVI.
ProtClustDB	CLSK560218.
Enzyme and pathway databases
BioCyc	SPNE171101:GJC8-1666-MONOMER.
Family and domain databases
Gene3D	2.160.20.10. 1 hit.
InterPro	IPR005877. Gpos_YSIRK. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Pfam	PF04650. YSIRK_signal. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
TIGRFAMs	TIGR01168. YSIRK_signal. 1 hit.
PROSITE	PS50847. GRAM_POS_ANCHORING. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFBA_STRR6

Accession

Primary (citable) accession number: Q8CYC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program