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Q8CY42

- RSH_BRUSU

UniProt

Q8CY42 - RSH_BRUSU

Protein

GTP pyrophosphokinase rsh

Gene

rsh

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment.1 Publication

    Catalytic activityi

    ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. GTP binding Source: UniProtKB-KW
    4. GTP diphosphokinase activity Source: UniProtKB-EC
    5. kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. guanosine tetraphosphate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTP pyrophosphokinase rsh (EC:2.7.6.5)
    Alternative name(s):
    (p)ppGpp synthase
    ATP:GTP 3'-pyrophosphotransferase
    Gene namesi
    Name:rsh
    Ordered Locus Names:BR0652, BS1330_I0648
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

    Pathology & Biotechi

    Disruption phenotypei

    Cells show morphological abnormalities such as branching and swelling forms during vegetative growth. It is unable to persist during stationary phase, presumably because of nutrient limitation occurring during this growth phase. It shows an important growth defect in human HeLa cells and in ovine macrophages MOCL3. At four weeks post infection the number of viable bacteria (deletion mutant) in mouse spleen is markedly reduced compared to the wild-type. The deletion mutant shows very low levels or absence of VirB at all time points of growth.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 750750GTP pyrophosphokinase rshPRO_0000322560Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi204722.BR0652.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CY42.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini45 – 144100HDAdd
    BLAST
    Domaini676 – 75075ACTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RelA/SpoT family.Curated
    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG0317.
    HOGENOMiHOG000018299.
    KOiK01139.
    OMAiESNAYSW.
    OrthoDBiEOG6SV551.

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view]
    PfamiPF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view]
    SUPFAMiSSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00691. spoT_relA. 1 hit.
    PROSITEiPS51671. ACT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CY42-1 [UniParc]FASTAAdd to Basket

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    MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL    50
    EVAAILTDMH LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL 100
    TKLKKLDLVS KKAVQAENLR KLLLAISEDV RVLLVKLADR LHNMRTLGVM 150
    CEDKRLRIAE ETMDIYAPLA GRMGMQDMRE ELEELAFRYI NPDAWRAVTD 200
    RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP WSVFRKMETK 250
    GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 300
    NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP 350
    HKISTETNAY AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR 400
    LIALPRGATP IDFAYAVHTD IGDSCVGAKV NGRIMPLMTE LKNGDEVDII 450
    RSKAQVPPAA WESLVATGKA RAAIRRATRS AVRKQYSGLG MRILERAFER 500
    AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSTDVV KAVYPDYQDT 550
    RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 600
    KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA 650
    YDDQPERWID VRWDIDDQMS ERFPARISVS AINSPGSLAK IAQIAAANDA 700
    NIHNLSMVRT APDFTEMIID VEVWDLKHLN RIISQLKESA SVSSAKRVNG 750
    Length:750
    Mass (Da):83,860
    Last modified:March 1, 2003 - v1
    Checksum:i195B752C18594104
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29581.1.
    CP002997 Genomic DNA. Translation: AEM17998.1.
    RefSeqiNP_697666.1. NC_004310.3.
    YP_005615490.1. NC_017251.1.

    Genome annotation databases

    EnsemblBacteriaiAAN29581; AAN29581; BR0652.
    AEM17998; AEM17998; BS1330_I0648.
    GeneIDi1166315.
    12136969.
    KEGGibms:BR0652.
    bsi:BS1330_I0648.
    PATRICi17789597. VBIBruSui107850_0665.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN29581.1 .
    CP002997 Genomic DNA. Translation: AEM17998.1 .
    RefSeqi NP_697666.1. NC_004310.3.
    YP_005615490.1. NC_017251.1.

    3D structure databases

    ProteinModelPortali Q8CY42.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204722.BR0652.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN29581 ; AAN29581 ; BR0652 .
    AEM17998 ; AEM17998 ; BS1330_I0648 .
    GeneIDi 1166315.
    12136969.
    KEGGi bms:BR0652.
    bsi:BS1330_I0648.
    PATRICi 17789597. VBIBruSui107850_0665.

    Phylogenomic databases

    eggNOGi COG0317.
    HOGENOMi HOG000018299.
    KOi K01139.
    OMAi ESNAYSW.
    OrthoDBi EOG6SV551.

    Miscellaneous databases

    PROi Q8CY42.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR002912. ACT_dom.
    IPR012675. Beta-grasp_dom.
    IPR003607. HD/PDEase_dom.
    IPR004811. RelA/Spo_fam.
    IPR007685. RelA_SpoT.
    IPR004095. TGS.
    IPR012676. TGS-like.
    [Graphical view ]
    Pfami PF04607. RelA_SpoT. 1 hit.
    PF02824. TGS. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    SM00954. RelA_SpoT. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00691. spoT_relA. 1 hit.
    PROSITEi PS51671. ACT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    3. "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB."
      Dozot M., Boigegrain R.-A., Delrue R.-M., Hallez R., Ouahrani-Bettache S., Danese I., Letesson J.-J., De Bolle X., Koehler S.
      Cell. Microbiol. 8:1791-1802(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN STRINGENT RESPONSE, DISRUPTION PHENOTYPE.
      Strain: 1330.

    Entry informationi

    Entry nameiRSH_BRUSU
    AccessioniPrimary (citable) accession number: Q8CY42
    Secondary accession number(s): G0K7Z3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3