Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CY42 (RSH_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTP pyrophosphokinase rsh

EC=2.7.6.5
Alternative name(s):
(p)ppGpp synthase
ATP:GTP 3'-pyrophosphotransferase
Gene names
Name:rsh
Ordered Locus Names:BR0652, BS1330_I0648
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length750 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. Ref.3

Catalytic activity

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate.

Disruption phenotype

Cells show morphological abnormalities such as branching and swelling forms during vegetative growth. It is unable to persist during stationary phase, presumably because of nutrient limitation occurring during this growth phase. It shows an important growth defect in human HeLa cells and in ovine macrophages MOCL3. At four weeks post infection the number of viable bacteria (deletion mutant) in mouse spleen is markedly reduced compared to the wild-type. The deletion mutant shows very low levels or absence of VirB at all time points of growth. Ref.3

Sequence similarities

Belongs to the RelA/SpoT family.

Contains 1 ACT domain.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 750750GTP pyrophosphokinase rsh
PRO_0000322560

Regions

Domain45 – 144100HD
Domain676 – 75075ACT

Sequences

Sequence LengthMass (Da)Tools
Q8CY42 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 195B752C18594104

FASTA75083,860
        10         20         30         40         50         60 
MMRQYELVER VQRYKPDVNE ALLNKAYVYA MQKHGSQKRA SGDPYFSHPL EVAAILTDMH 

        70         80         90        100        110        120 
LDEATIAIAL LHDTIEDTTA TRQEIDQLFG PEIGKLVEGL TKLKKLDLVS KKAVQAENLR 

       130        140        150        160        170        180 
KLLLAISEDV RVLLVKLADR LHNMRTLGVM CEDKRLRIAE ETMDIYAPLA GRMGMQDMRE 

       190        200        210        220        230        240 
ELEELAFRYI NPDAWRAVTD RLAELLEKNR GLLQKIETDL SEIFEKNGIK ASVKSRQKKP 

       250        260        270        280        290        300 
WSVFRKMETK GLSFEQLSDI FGFRVMVDTV QDCYRALGLI HTTWSMVPGR FKDYISTPKQ 

       310        320        330        340        350        360 
NDYRSIHTTI IGPSRQRIEL QIRTREMDEI AEFGVAAHSI YKDRGSANNP HKISTETNAY 

       370        380        390        400        410        420 
AWLRQTIEQL SEGDNPEEFL EHTKLELFQD QVFCFTPKGR LIALPRGATP IDFAYAVHTD 

       430        440        450        460        470        480 
IGDSCVGAKV NGRIMPLMTE LKNGDEVDII RSKAQVPPAA WESLVATGKA RAAIRRATRS 

       490        500        510        520        530        540 
AVRKQYSGLG MRILERAFER AGKPFSKDIL KPGLPRLARK DVEDVLAAVG RGELPSTDVV 

       550        560        570        580        590        600 
KAVYPDYQDT RVTTQNNPAK AGEKGWFNIQ NAAGMIFKVP EGGEGAAAKV DPAATTPKPG 

       610        620        630        640        650        660 
KRALPIRGTN PDLPVRFAPE GAVPGDRIVG ILQPGAGITI YPIQSPALTA YDDQPERWID 

       670        680        690        700        710        720 
VRWDIDDQMS ERFPARISVS AINSPGSLAK IAQIAAANDA NIHNLSMVRT APDFTEMIID 

       730        740        750 
VEVWDLKHLN RIISQLKESA SVSSAKRVNG 

« Hide

References

« Hide 'large scale' references
[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[3]"The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the type IV secretion system virB."
Dozot M., Boigegrain R.-A., Delrue R.-M., Hallez R., Ouahrani-Bettache S., Danese I., Letesson J.-J., De Bolle X., Koehler S.
Cell. Microbiol. 8:1791-1802(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN STRINGENT RESPONSE, DISRUPTION PHENOTYPE.
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29581.1.
CP002997 Genomic DNA. Translation: AEM17998.1.
RefSeqNP_697666.1. NC_004310.3.
YP_005615490.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8CY42.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR0652.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN29581; AAN29581; BR0652.
AEM17998; AEM17998; BS1330_I0648.
GeneID1166315.
12136969.
KEGGbms:BR0652.
bsi:BS1330_I0648.
PATRIC17789597. VBIBruSui107850_0665.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0317.
HOGENOMHOG000018299.
KOK01139.
OMAESNAYSW.
OrthoDBEOG6SV551.
ProtClustDBCLSK2754108.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR026020. (p)ppGpp_Synthase.
IPR002912. ACT_dom.
IPR012675. Beta-grasp_dom.
IPR003607. HD/PDEase_dom.
IPR004811. RelA/Spo_fam.
IPR007685. RelA_SpoT.
IPR004095. TGS.
IPR012676. TGS-like.
[Graphical view]
PANTHERPTHR21262:SF1. PTHR21262:SF1. 1 hit.
PfamPF04607. RelA_SpoT. 1 hit.
PF02824. TGS. 1 hit.
[Graphical view]
SMARTSM00471. HDc. 1 hit.
SM00954. RelA_SpoT. 1 hit.
[Graphical view]
SUPFAMSSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00691. spoT_relA. 1 hit.
PROSITEPS51671. ACT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ8CY42.

Entry information

Entry nameRSH_BRUSU
AccessionPrimary (citable) accession number: Q8CY42
Secondary accession number(s): G0K7Z3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names