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Protein

tRNA dimethylallyltransferase

Gene

miaA

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A).UniRule annotation

Catalytic activityi

Dimethylallyl diphosphate + adenine(37) in tRNA = diphosphate + N(6)-dimethylallyladenine(37) in tRNA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 218ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Magnesium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA dimethylallyltransferaseUniRule annotation (EC:2.5.1.75UniRule annotation)
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMAPP:tRNA dimethylallyltransferaseUniRule annotation
Short name:
DMATaseUniRule annotation
Isopentenyl-diphosphate:tRNA isopentenyltransferaseUniRule annotation
Short name:
IPP transferaseUniRule annotation
Short name:
IPPTUniRule annotation
Short name:
IPTaseUniRule annotation
Gene namesi
Name:miaAUniRule annotation
Ordered Locus Names:BR1390, BS1330_I1384
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310tRNA dimethylallyltransferasePRO_0000163889Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei105 – 1051Interaction with substrate tRNAUniRule annotation
Sitei127 – 1271Interaction with substrate tRNAUniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ8CY40.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni16 – 216Substrate bindingUniRule annotation
Regioni39 – 424Interaction with substrate tRNAUniRule annotation
Regioni163 – 1675Interaction with substrate tRNAUniRule annotation

Sequence similaritiesi

Belongs to the IPP transferase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000039996.
KOiK00791.
OMAiTWFRNQT.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CY40-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDAVKNAI LIAGPTASGK SALAIRMAKA TGGFIVNTDS MQVYGVLDLL
60 70 80 90 100
TARPSRADLA EAEHFLYGHV PPSSTYSTGK WFEDVEALLG RCGLQGRVPI
110 120 130 140 150
FVGGTGLYFR ALLGGLSQMP EVSAQVRDHW RGRMEAEGAK ALHAVLCVRD
160 170 180 190 200
PAIAAALQPS DSQRIVRALE VLESTGKSLL EWQKVKGRAL VDDQSAQKIV
210 220 230 240 250
LRPDRAWLGE RIARRFSAMW AEGAIDEVRA LLALDLDPAL PAMKAIGVRE
260 270 280 290 300
VSAFLAETMS REEAIERSVI ATRQYAKRQS TWFRNQLGED WRVYASGEEV
310
FQGGSFRDPQ
Length:310
Mass (Da):33,943
Last modified:March 1, 2003 - v1
Checksum:i8955CC4EDB0668DD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30303.1.
CP002997 Genomic DNA. Translation: AEM18719.1.
RefSeqiWP_006190580.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN30303; AAN30303; BR1390.
AEM18719; AEM18719; BS1330_I1384.
KEGGibms:BR1390.
bsi:BS1330_I1384.
PATRICi17791139. VBIBruSui107850_1416.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30303.1.
CP002997 Genomic DNA. Translation: AEM18719.1.
RefSeqiWP_006190580.1. NZ_KN046804.1.

3D structure databases

ProteinModelPortaliQ8CY40.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30303; AAN30303; BR1390.
AEM18719; AEM18719; BS1330_I1384.
KEGGibms:BR1390.
bsi:BS1330_I1384.
PATRICi17791139. VBIBruSui107850_1416.

Phylogenomic databases

HOGENOMiHOG000039996.
KOiK00791.
OMAiTWFRNQT.

Miscellaneous databases

PROiQ8CY40.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00185. IPP_trans. 1 hit.
InterProiIPR018022. IPP_trans.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01715. IPPT. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00174. miaA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMIAA_BRUSU
AccessioniPrimary (citable) accession number: Q8CY40
Secondary accession number(s): G0KB73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.