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Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
Active sitei114 – 1141NucleophileUniRule annotation
Binding sitei138 – 1381ATP; via amide nitrogenUniRule annotation
Sitei139 – 1391Interaction with tRNAUniRule annotation
Active sitei210 – 2101Cysteine persulfide intermediateUniRule annotation
Sitei352 – 3521Interaction with tRNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 278ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. sulfurtransferase activity Source: InterPro
  3. tRNA binding Source: UniProtKB-KW

GO - Biological processi

  1. tRNA modification Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.-UniRule annotation)
Gene namesi
Name:mnmAUniRule annotation
Synonyms:trmU
Ordered Locus Names:BR1591, BS1330_I1585
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398tRNA-specific 2-thiouridylase MnmAPRO_0000121614Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi114 ↔ 210AlternateUniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi204722.BR1591.

Structurei

3D structure databases

ProteinModelPortaliQ8CY38.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni160 – 1623Interaction with tRNAUniRule annotation

Sequence similaritiesi

Belongs to the MnmA/TRMU family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0482.
HOGENOMiHOG000218045.
KOiK00566.
OMAiEPLYVVH.
OrthoDBiEOG6RZB5H.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
PfamiPF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00420. trmU. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CY38-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLNSLDLPG KPEDTRVVVA MSGGVDSSVV AGILKREGYD VVGVTLQLYD
60 70 80 90 100
HGAAVHRAGS CCAGQDIEDA RRVSESLGIP HYVLDYEARF REAVIDPFAN
110 120 130 140 150
SYVSGETPIP CVSCNQTVKF ADLLQTARDL GADALATGHY IRSRANGAHR
160 170 180 190 200
ALYRPVDTDR DQSYFLFATT QEQIDYLRFP LGHLPKAQVR EIAEELGLTV
210 220 230 240 250
AKKQDSQDIC FVPQGKYSDI ISRLKPEAAN PGDIVHIDGR TLGRHDGIVH
260 270 280 290 300
YTVGQRRGIG VATGEALYVV HLDAANARVI VGPREALETH KVFLRDVNWL
310 320 330 340 350
GDTPIADLPK SGMEVFAKVR STRPPRPAVL RHADGQTWVE LVDGESGIAP
360 370 380 390
GQACVLYSDD SNAARVFGGG FIGRSEREPQ AEEMLRRLMA NADKASAA
Length:398
Mass (Da):43,229
Last modified:March 1, 2003 - v1
Checksum:i937367AC3BAB9ED3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30496.1.
CP002997 Genomic DNA. Translation: AEM18912.1.
RefSeqiNP_698581.1. NC_004310.3.
YP_005616404.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN30496; AAN30496; BR1591.
AEM18912; AEM18912; BS1330_I1585.
GeneIDi1167274.
12137345.
KEGGibms:BR1591.
bsi:BS1330_I1585.
PATRICi17791548. VBIBruSui107850_1618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN30496.1.
CP002997 Genomic DNA. Translation: AEM18912.1.
RefSeqiNP_698581.1. NC_004310.3.
YP_005616404.1. NC_017251.1.

3D structure databases

ProteinModelPortaliQ8CY38.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR1591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN30496; AAN30496; BR1591.
AEM18912; AEM18912; BS1330_I1585.
GeneIDi1167274.
12137345.
KEGGibms:BR1591.
bsi:BS1330_I1585.
PATRICi17791548. VBIBruSui107850_1618.

Phylogenomic databases

eggNOGiCOG0482.
HOGENOMiHOG000218045.
KOiK00566.
OMAiEPLYVVH.
OrthoDBiEOG6RZB5H.

Family and domain databases

Gene3Di2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
InterProiIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERiPTHR11933. PTHR11933. 1 hit.
PfamiPF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00420. trmU. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiMNMA_BRUSU
AccessioniPrimary (citable) accession number: Q8CY38
Secondary accession number(s): G0K6C8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.