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Q8CY38 (MNMA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-specific 2-thiouridylase MnmA

EC=2.8.1.-
Gene names
Name:mnmA
Synonyms:trmU
Ordered Locus Names:BR1591, BS1330_I1585
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34 By similarity. HAMAP-Rule MF_00144

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00144.

Sequence similarities

Belongs to the MnmA/TRMU family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
tRNA-binding
   Molecular functionTransferase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

sulfurtransferase activity

Inferred from electronic annotation. Source: InterPro

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398tRNA-specific 2-thiouridylase MnmA HAMAP-Rule MF_00144
PRO_0000121614

Regions

Nucleotide binding20 – 278ATP By similarity
Region160 – 1623Interaction with tRNA By similarity

Sites

Active site1141Nucleophile By similarity
Active site2101Cysteine persulfide intermediate By similarity
Binding site461ATP; via amide nitrogen and carbonyl oxygen By similarity
Binding site1381ATP; via amide nitrogen By similarity
Site1391Interaction with tRNA By similarity
Site3521Interaction with tRNA By similarity

Amino acid modifications

Disulfide bond114 ↔ 210Alternate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CY38 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 937367AC3BAB9ED3

FASTA39843,229
        10         20         30         40         50         60 
MSLNSLDLPG KPEDTRVVVA MSGGVDSSVV AGILKREGYD VVGVTLQLYD HGAAVHRAGS 

        70         80         90        100        110        120 
CCAGQDIEDA RRVSESLGIP HYVLDYEARF REAVIDPFAN SYVSGETPIP CVSCNQTVKF 

       130        140        150        160        170        180 
ADLLQTARDL GADALATGHY IRSRANGAHR ALYRPVDTDR DQSYFLFATT QEQIDYLRFP 

       190        200        210        220        230        240 
LGHLPKAQVR EIAEELGLTV AKKQDSQDIC FVPQGKYSDI ISRLKPEAAN PGDIVHIDGR 

       250        260        270        280        290        300 
TLGRHDGIVH YTVGQRRGIG VATGEALYVV HLDAANARVI VGPREALETH KVFLRDVNWL 

       310        320        330        340        350        360 
GDTPIADLPK SGMEVFAKVR STRPPRPAVL RHADGQTWVE LVDGESGIAP GQACVLYSDD 

       370        380        390 
SNAARVFGGG FIGRSEREPQ AEEMLRRLMA NADKASAA 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN30496.1.
CP002997 Genomic DNA. Translation: AEM18912.1.
RefSeqNP_698581.1. NC_004310.3.
YP_005616404.1. NC_017251.1.

3D structure databases

ProteinModelPortalQ8CY38.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR1591.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN30496; AAN30496; BR1591.
AEM18912; AEM18912; BS1330_I1585.
GeneID1167274.
12137345.
KEGGbms:BR1591.
bsi:BS1330_I1585.
PATRIC17791548. VBIBruSui107850_1618.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0482.
HOGENOMHOG000218045.
KOK00566.
OMAMQYTIGK.
OrthoDBEOG6RZB5H.
ProtClustDBPRK00143.

Family and domain databases

Gene3D2.30.30.280. 1 hit.
3.40.50.620. 1 hit.
HAMAPMF_00144. tRNA_thiouridyl_MnmA.
InterProIPR023382. Adenine_a_hdrlase_dom.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004506. tRNA-specific_2-thiouridylase.
[Graphical view]
PANTHERPTHR11933. PTHR11933. 1 hit.
PfamPF03054. tRNA_Me_trans. 1 hit.
[Graphical view]
TIGRFAMsTIGR00420. trmU. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMNMA_BRUSU
AccessionPrimary (citable) accession number: Q8CY38
Secondary accession number(s): G0K6C8
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names