Q8CY38 (MNMA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA-specific 2-thiouridylase MnmA
EC=2.8.1.-

Gene names

Name:	mnmA
Synonyms:	trmU
Ordered Locus Names:	BR1591, BS1330_I1585

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34 By similarity. HAMAP-Rule MF_00144
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the MnmA/TRMU family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Transferase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW sulfurtransferase activity Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	tRNA-specific 2-thiouridylase MnmA HAMAP-Rule MF_00144		PRO_0000121614
Regions
	Nucleotide binding	20 – 27	8	ATP By similarity
	Region	160 – 162	3	Interaction with tRNA By similarity
Sites
	Active site	114	1	Nucleophile By similarity
	Active site	210	1	Cysteine persulfide intermediate By similarity
	Binding site	46	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	138	1	ATP; via amide nitrogen By similarity
	Site	139	1	Interaction with tRNA By similarity
	Site	352	1	Interaction with tRNA By similarity
Amino acid modifications
	Disulfide bond	114 ↔ 210		Alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CY38 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 937367AC3BAB9ED3	FASTA	398	43,229
	10 20 30 40 50 60 MSLNSLDLPG KPEDTRVVVA MSGGVDSSVV AGILKREGYD VVGVTLQLYD HGAAVHRAGS 70 80 90 100 110 120 CCAGQDIEDA RRVSESLGIP HYVLDYEARF REAVIDPFAN SYVSGETPIP CVSCNQTVKF 130 140 150 160 170 180 ADLLQTARDL GADALATGHY IRSRANGAHR ALYRPVDTDR DQSYFLFATT QEQIDYLRFP 190 200 210 220 230 240 LGHLPKAQVR EIAEELGLTV AKKQDSQDIC FVPQGKYSDI ISRLKPEAAN PGDIVHIDGR 250 260 270 280 290 300 TLGRHDGIVH YTVGQRRGIG VATGEALYVV HLDAANARVI VGPREALETH KVFLRDVNWL 310 320 330 340 350 360 GDTPIADLPK SGMEVFAKVR STRPPRPAVL RHADGQTWVE LVDGESGIAP GQACVLYSDD 370 380 390 SNAARVFGGG FIGRSEREPQ AEEMLRRLMA NADKASAA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014291 Genomic DNA. Translation: AAN30496.1. CP002997 Genomic DNA. Translation: AEM18912.1.
RefSeq	NP_698581.1. NC_004310.3. YP_005616404.1. NC_017251.1.
3D structure databases
ProteinModelPortal	Q8CY38.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BR1591.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN30496; AAN30496; BR1591. AEM18912; AEM18912; BS1330_I1585.
GeneID	1167274. 12137345.
KEGG	bms:BR1591. bsi:BS1330_I1585.
PATRIC	17791548. VBIBruSui107850_1618.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0482.
HOGENOM	HOG000218045.
KO	K00566.
OMA	HASEKKH.
ProtClustDB	PRK00143.
Family and domain databases
Gene3D	2.30.30.280. 1 hit. 3.40.50.620. 1 hit.
HAMAP	MF_00144. tRNA_thiouridyl_MnmA.
InterPro	IPR023382. Adenine_a_hdrlase_dom. IPR014729. Rossmann-like_a/b/a_fold. IPR004506. tRNA-specific_2-thiouridylase. [Graphical view]
PANTHER	PTHR11933. PTHR11933. 1 hit.
Pfam	PF03054. tRNA_Me_trans. 1 hit. [Graphical view]
TIGRFAMs	TIGR00420. trmU. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNMA_BRUSU

Accession

Primary (citable) accession number: Q8CY38
Secondary accession number(s): G0K6C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program