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Q8CY38

- MNMA_BRUSU

UniProt

Q8CY38 - MNMA_BRUSU

Protein

tRNA-specific 2-thiouridylase MnmA

Gene

mnmA

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 69 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei46 – 461ATP; via amide nitrogen and carbonyl oxygenUniRule annotation
    Active sitei114 – 1141NucleophileUniRule annotation
    Binding sitei138 – 1381ATP; via amide nitrogenUniRule annotation
    Sitei139 – 1391Interaction with tRNAUniRule annotation
    Active sitei210 – 2101Cysteine persulfide intermediateUniRule annotation
    Sitei352 – 3521Interaction with tRNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi20 – 278ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. sulfurtransferase activity Source: InterPro
    3. tRNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding, RNA-binding, tRNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-specific 2-thiouridylase MnmAUniRule annotation (EC:2.8.1.-UniRule annotation)
    Gene namesi
    Name:mnmAUniRule annotation
    Synonyms:trmU
    Ordered Locus Names:BR1591, BS1330_I1585
    OrganismiBrucella suis biovar 1 (strain 1330)
    Taxonomic identifieri204722 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
    ProteomesiUP000000824: Chromosome I, UP000007104: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 398398tRNA-specific 2-thiouridylase MnmAPRO_0000121614Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi114 ↔ 210AlternateUniRule annotation

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi204722.BR1591.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CY38.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni160 – 1623Interaction with tRNAUniRule annotation

    Sequence similaritiesi

    Belongs to the MnmA/TRMU family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0482.
    HOGENOMiHOG000218045.
    KOiK00566.
    OMAiYISHNEN.
    OrthoDBiEOG6RZB5H.

    Family and domain databases

    Gene3Di2.30.30.280. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00144. tRNA_thiouridyl_MnmA.
    InterProiIPR023382. Adenine_a_hdrlase_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view]
    PANTHERiPTHR11933. PTHR11933. 1 hit.
    PfamiPF03054. tRNA_Me_trans. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00420. trmU. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8CY38-1 [UniParc]FASTAAdd to Basket

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    MSLNSLDLPG KPEDTRVVVA MSGGVDSSVV AGILKREGYD VVGVTLQLYD    50
    HGAAVHRAGS CCAGQDIEDA RRVSESLGIP HYVLDYEARF REAVIDPFAN 100
    SYVSGETPIP CVSCNQTVKF ADLLQTARDL GADALATGHY IRSRANGAHR 150
    ALYRPVDTDR DQSYFLFATT QEQIDYLRFP LGHLPKAQVR EIAEELGLTV 200
    AKKQDSQDIC FVPQGKYSDI ISRLKPEAAN PGDIVHIDGR TLGRHDGIVH 250
    YTVGQRRGIG VATGEALYVV HLDAANARVI VGPREALETH KVFLRDVNWL 300
    GDTPIADLPK SGMEVFAKVR STRPPRPAVL RHADGQTWVE LVDGESGIAP 350
    GQACVLYSDD SNAARVFGGG FIGRSEREPQ AEEMLRRLMA NADKASAA 398
    Length:398
    Mass (Da):43,229
    Last modified:March 1, 2003 - v1
    Checksum:i937367AC3BAB9ED3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN30496.1.
    CP002997 Genomic DNA. Translation: AEM18912.1.
    RefSeqiNP_698581.1. NC_004310.3.
    YP_005616404.1. NC_017251.1.

    Genome annotation databases

    EnsemblBacteriaiAAN30496; AAN30496; BR1591.
    AEM18912; AEM18912; BS1330_I1585.
    GeneIDi1167274.
    12137345.
    KEGGibms:BR1591.
    bsi:BS1330_I1585.
    PATRICi17791548. VBIBruSui107850_1618.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014291 Genomic DNA. Translation: AAN30496.1 .
    CP002997 Genomic DNA. Translation: AEM18912.1 .
    RefSeqi NP_698581.1. NC_004310.3.
    YP_005616404.1. NC_017251.1.

    3D structure databases

    ProteinModelPortali Q8CY38.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 204722.BR1591.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN30496 ; AAN30496 ; BR1591 .
    AEM18912 ; AEM18912 ; BS1330_I1585 .
    GeneIDi 1167274.
    12137345.
    KEGGi bms:BR1591.
    bsi:BS1330_I1585.
    PATRICi 17791548. VBIBruSui107850_1618.

    Phylogenomic databases

    eggNOGi COG0482.
    HOGENOMi HOG000218045.
    KOi K00566.
    OMAi YISHNEN.
    OrthoDBi EOG6RZB5H.

    Family and domain databases

    Gene3Di 2.30.30.280. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00144. tRNA_thiouridyl_MnmA.
    InterProi IPR023382. Adenine_a_hdrlase_dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR004506. tRNA-specific_2-thiouridylase.
    [Graphical view ]
    PANTHERi PTHR11933. PTHR11933. 1 hit.
    Pfami PF03054. tRNA_Me_trans. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00420. trmU. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 1330.

    Entry informationi

    Entry nameiMNMA_BRUSU
    AccessioniPrimary (citable) accession number: Q8CY38
    Secondary accession number(s): G0K6C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 69 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Brucella suis
      Brucella suis (strain 1330): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3