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Q8CY26 (TRMD_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228
Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase
Gene names
Name:trmD
Ordered Locus Names:CE1956
OrganismCorynebacterium efficiens [Complete proteome] [HAMAP]
Taxonomic identifier152794 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length291 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605

Catalytic activity

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605

Subunit structure

Homodimer By similarity. HAMAP MF_00605

Subcellular location

Cytoplasm Potential HAMAP MF_00605.

Sequence similarities

Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

tRNA (guanine-N1-)-methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 291291tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
PRO_0000060364

Regions

Region184 – 1896S-adenosyl-L-methionine binding By similarity

Sites

Binding site1601S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CY26 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 3AEF271293003E5B

FASTA29132,235
        10         20         30         40         50         60 
MNAVTSANNN TDNRRLRLDV VSIFPEYLDP LRHALLGKAI EDGYLEVGVH DLRDWATGGH 

        70         80         90        100        110        120 
KAVDDTPYGG GPGMVMKPEV WGPALDDVAA GRVAGWELES ATPHRNVARH DELAGVDKHA 

       130        140        150        160        170        180 
YEGEDADLPL LLVPTPAGKP FTQADAQAWS NENHIVFACG RYEGIDQRVI EDARNRYRVR 

       190        200        210        220        230        240 
EVSIGDYVLI GGEVAVLVIA EAVVRLIPGV LGNRRSHEED SFSDGLLEGP SYTKPRTWRG 

       250        260        270        280        290 
LDVPEVLFSG NHARVDRWRR DQALLRTQRV RPELLDAVEL TTEDRKVLGL D

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000035 Genomic DNA. Translation: BAC18766.1.
RefSeqNP_738566.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8CY26.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1034676.
GenomeReviewsGene locus CE1956 in contig BA000035_GR.
KEGGcef:CE1956.
NMPDRfig|196164.1.peg.1956.
PATRIC21490049. VBICorEff9312_1939.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG285805.
OMAVCGRFEG.
PhylomeDBQ8CY26.
ProtClustDBPRK00026.

Enzyme and pathway databases

BioCycCEFF196164:CE1956-MONOMER.

Family and domain databases

HAMAPMF_00605. TrmD.
[Tree]
InterProIPR016009. tRNA_m1G_MeTrfase.
IPR002649. tRNA_m1G_MeTrfase_bac.
IPR023148. tRNA_m1G_MeTrfase_C.
[Graphical view]
Gene3DG3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KOK00554.
PANTHERPTHR10056. PTHR10056. 1 hit.
PfamPF01746. tRNA_m1G_MT. 2 hits.
[Graphical view]
PIRSFPIRSF000386. tRNA_mtase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRMD_COREF
AccessionPrimary (citable) accession number: Q8CY26
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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