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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131PhosphateUniRule annotation
Sitei168 – 1681Important for substrate specificityUniRule annotation
Binding sitei186 – 1861Substrate; via amide nitrogenUniRule annotation
Binding sitei187 – 1871PhosphateUniRule annotation
Sitei223 – 2231Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciLINT189518:GJBB-3366-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:LA_4248
OrganismiLeptospira interrogans serogroup Icterohaemorrhagiae serovar Lai (strain 56601)
Taxonomic identifieri189518 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesLeptospiraceaeLeptospira
ProteomesiUP000001408 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287S-methyl-5'-thioadenosine phosphorylasePRO_0000184550Add
BLAST

Proteomic databases

PaxDbiQ8CXR2.

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi189518.LA4248.

Structurei

3D structure databases

ProteinModelPortaliQ8CXR2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate bindingUniRule annotation
Regioni210 – 2123Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
InParanoidiQ8CXR2.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG6KHFXC.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CXR2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYNVRAAII GGTGLYSLEG MELIEEIFPD TPWGKPSDKI KIGKYKGKLI
60 70 80 90 100
AFLPRHGIGH FLSPPEVPNH ANICALKQLG VEEIVAFSSV GSLREEIKPL
110 120 130 140 150
DFVLPSQIID RTRFRNSTYF GNGVVAHAPF AEPFSPNLSK RIAQTAKKIG
160 170 180 190 200
LEIHLDKTLV CMEGPLFSTK AESHLYRSWG ADIINMTVLP EAKLAREAEI
210 220 230 240 250
AYQMICMSTD YDCWREGEES VTVEMVIANL TKNAETAKKL LSELIHVLGN
260 270 280
GDDLSLKNST RYSIITAPEK RNSETVKKLR VLFPEYF
Length:287
Mass (Da):32,031
Last modified:February 28, 2003 - v1
Checksum:i2830C5FB62F3AE88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010300 Genomic DNA. Translation: AAN51446.1.
RefSeqiNP_714428.1. NC_004342.2.

Genome annotation databases

EnsemblBacteriaiAAN51446; AAN51446; LA_4248.
GeneIDi1153590.
KEGGilil:LA_4248.
PATRICi22389337. VBILepInt91350_4221.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010300 Genomic DNA. Translation: AAN51446.1.
RefSeqiNP_714428.1. NC_004342.2.

3D structure databases

ProteinModelPortaliQ8CXR2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi189518.LA4248.

Proteomic databases

PaxDbiQ8CXR2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN51446; AAN51446; LA_4248.
GeneIDi1153590.
KEGGilil:LA_4248.
PATRICi22389337. VBILepInt91350_4221.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
InParanoidiQ8CXR2.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG6KHFXC.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.
BioCyciLINT189518:GJBB-3366-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 56601.

Entry informationi

Entry nameiMTAP_LEPIN
AccessioniPrimary (citable) accession number: Q8CXR2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 18, 2004
Last sequence update: February 28, 2003
Last modified: January 6, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.