Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot Q8CXH8 (CARA_OCEIH)

Last modified June 16, 2009. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name: carA
Synonyms: pyrAA
Ordered Locus Names: OB1490
OrganismOceanobacillus iheyensis [Complete proteome] [HAMAP]
Taxonomic identifier182710 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Hide | Top

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Sequence similarities

Belongs to the carA family.

Contains 1 glutamine amidotransferase type-1 domain.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_0000112300

Regions

Domain172 – 359188Glutamine amidotransferase type-1
Region1 – 168168CPSase HAMAP MF_01209

Sites

Active site2471Nucleophile By similarity
Active site3321 By similarity
Active site3341 By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Q8CXH8-1 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 10C38A4340928299

FASTA36640,740
        10         20         30         40         50         60 
MKKRKLILED GTVFNGTAFG SDAESSGEIV FNTGMTGYQE VITDPSYCGQ FVTLTYPLIG 

        70         80         90        100        110        120 
NYGINRDDFE TVTPFIHGLV VKEYSEFPSN FRNEETLDEF LQAHNIPGIA NIDTRKLTRI 

       130        140        150        160        170        180 
IRKHGTMRAV MVDEQKNEQH VIEQLRLAEM PRDQVKRTST IKPYVVPGRG LRVVMVDFGA 

       190        200        210        220        230        240 
KHGILRELTR RDCHITVVPH NYSAEAILRL KPDGIMLTNG PGDPKDVPEA IEMIKQLLGQ 

       250        260        270        280        290        300 
IPIFGICLGH QLLALACGAD TEKMKFGHRG ANHPVKDLLA GKTYLTSQNH SYAVNVSSLV 

       310        320        330        340        350        360 
NTDLELTQIA LNDDTVEGIR HTAFPAFSVQ YHPEASPGPE DTNFLFDEFL NLIKASKVKQ 


GGEVYA

« Hide

Hide | Top

References

[1]"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments."
Takami H., Takaki Y., Uchiyama I.
Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Hide | Top

Cross-references

Sequence databases

BA000028 Genomic DNA. Translation: BAC13446.1.
RefSeqNP_692411.1.

3D structure databases

HSSPHSSP built from PDB template 1CE8 based on UniProtKB P00907.
ModBaseSearch...

Genome annotation databases

GeneID1017748.
GenomeReviewsGene locus OB1490 in contig BA000028_GR.
KEGGoih:OB1490.
NMPDRfig|221109.1.peg.1492.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8CXH8.
OMAQ8CXH8. EADIPFF.

Enzyme and pathway databases

BioCycOIHE221109:OB1490-MON.
BRENDA6.3.5.5. 278212.

Family and domain databases

HAMAPMF_01209.
[Tree]
InterProIPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
[Graphical view]
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
PRINTSPR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameCARA_OCEIH
AccessionPrimary (citable) accession number: Q8CXH8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 6, 2003
Last sequence update: March 1, 2003
Last modified: June 16, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents