Reviewed, UniProtKB/Swiss-Prot Q8CXC9 (GPR_OCEIH)

Names and origin

Protein names

Recommended name:
    Germination protease
    EC=3.4.24.78
Alternative name(s):
    Spore protease
    GPR endopeptidase
    Germination proteinase

Gene names

Name:	gpr
Ordered Locus Names:	OB1975

Organism

Oceanobacillus iheyensis [Complete proteome] [HAMAP]

Taxonomic identifier

182710 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Initiates the rapid degradation of small, acid-soluble proteins during spore germination By similarity.
Catalytic activity	Endopeptidase action with P4 Glu or Asp, P1 preferably Glu > Asp, P1' hydrophobic and P2' Ala. HAMAP MF_00626
Subunit structure	Homotetramer By similarity.
Post-translational modification	Autoproteolytically processed. The inactive tetrameric zymogen termed p46 autoprocesses to a smaller form termed p41, which is active only during spore germination By similarity.
Sequence similarities	Belongs to the peptidase A25 family.

Ontologies

Keywords
Molecular function	Hydrolase Protease
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP spore germination Inferred from electronic annotation. Source: HAMAP
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 13	13	By similarity		PRO_0000026878
	Chain	14 – 367	354	Germination protease HAMAP MF_00626		PRO_0000026879

Sequences

	Sequence		Length	Mass (Da)
	Q8CXC9-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3F4985F73F2CE5C7	FASTA	367	40,467
	10 20 30 40 50 60 MEEQQIPFQV RTDLAIEAKD MYTESKPEET NDKEIKGVTF KERSVKDIKV SYVDIDEEGE 70 80 90 100 110 120 KLLGKKPGSY VTIYADGVKK QDTDRQGQAA QVLAKELEDL MRKNNVTKES TCLVVGLGNW 130 140 150 160 170 180 NVTPDALGPM TVEKVLVTSH LFRLQYETVA QGYRDVAAVT PGVMGVTGIE TSDIIFGIVE 190 200 210 220 230 240 KYKPDLVIAV DALASRSINR VNETIQLSDT GIHPGSGVGN KRKEISKKTL GIPVIAIGVP 250 260 270 280 290 300 TVVDAVTITS DTIDYVLKHF GREWKEKDDP SKSLTPAGMS FGNRKLTDED LPNMEKRKTV 310 320 330 340 350 360 LGIVGELSDE EKRKLITEVL TPLGHNLMVT PKEVDGFMID MAEVLANGIN AALHEKVDVD NFANYSR

References

[1]

"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000028 Genomic DNA. Translation: BAC13931.1.
RefSeq	NP_692896.1.
3D structure databases
HSSP	HSSP built from PDB template 1C8B based on UniProtKB P22321.
ModBase	Search...
Protein family/group databases
MEROPS	A25.001.
Genome annotation databases
GeneID	1018452.
GenomeReviews	Gene locus OB1975 in contig BA000028_GR.
KEGG	oih:OB1975.
NMPDR	fig|221109.1.peg.1975.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CXC9.
OMA	Q8CXC9. LMVTRHL.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1975-MON.
BRENDA	3.4.24.78. 278212.
Family and domain databases
HAMAP	MF_00626. [Tree]
InterPro	IPR005080. Peptidase_A25. [Graphical view]
Gene3D	G3DSA:3.40.50.10100. Peptidase_A25. 1 hit.
Pfam	PF03418. Peptidase_A25. 1 hit. [Graphical view]
PIRSF	PIRSF019549. Peptidase_A25. 1 hit.
ProDom	PD041835. Peptidase_A25. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01441. GPR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPR_OCEIH

Accession

Primary (citable) accession number: Q8CXC9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)