Q8CX61 (DHA_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine dehydrogenase
EC=1.4.1.1

Gene names

Name:	ald
Ordered Locus Names:	OB3225

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is a key factor in the assimilation of L-alanine as an energy source through the tricarboxylic acid cycle during sporulation By similarity.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alanine dehydrogenase		PRO_0000198993
Regions
	Nucleotide binding	169 – 199	31	NAD By similarity
Sites
	Active site	96	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8CX61 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EAC9F969A2041BC0	FASTA	376	39,619
	10 20 30 40 50 60 MIIGVPKEIK NNENRVAMTP AGVVHLINAG HTVQIEKGAG LGSNFADAEY KEAGAELIDS 70 80 90 100 110 120 AASVWENADM IMKVKEPLSS EYKYFRKGLI LFTYLHLAAA PELTKALVDS EVTAIAYETI 130 140 150 160 170 180 TVNGTLPLLT PMSEVAGRMA TQIGAQYLEK SEGGKGILLG GIPGVSRGKV TVIGGGVVGT 190 200 210 220 230 240 HAAKIALGLG AEVTIIDLNP VRLRQLDDIF GSSIQTLMSN PYNIAEAVKD SDLVIGSVLI 250 260 270 280 290 300 PGRKAPKLVT DEMIQSMQPG SVLVDVAIDQ GGNFETVDHP TTHDEPIYVK HDVLHYAVAN 310 320 330 340 350 360 IPGAVPRTAT VGLTNVTVPY AVQIASKGAV KAIQDNPAIL TGVNVMNGKV TYEAVAADLG 370 YDFVSPEDAI KDAELV

References

[1]	"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC15181.1.
RefSeq	NP_694147.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8CX61.
SMR	Q8CX61. Positions 1-357.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1016523.
GenomeReviews	Gene locus OB3225 in contig BA000028_GR.
KEGG	oih:OB3225.
NMPDR	fig|221109.1.peg.3227.
PATRIC	22797548. VBIOceIhe82024_3278.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289735.
OMA	HEYRVAA.
PhylomeDB	Q8CX61.
Enzyme and pathway databases
BioCyc	OIHE221109:OB3225-MONOMER.
Family and domain databases
InterPro	IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR008141. Ala_DH/Pyr_transHydgase. IPR007886. AlaDH/PNT_N. IPR007698. AlaDH/PNT_NAD(H)-bd. [Graphical view]
KO	K00259.
PANTHER	PTHR10160:SF3. PTHR10160:SF3. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
PIRSF	PIRSF000183. Alanine_dh. 1 hit.
SMART	SM01002. AlaDh_PNT_C. 1 hit. SM01003. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. AlaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA_OCEIH

Accession

Primary (citable) accession number: Q8CX61

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program