Q8CWY4 (NADE_STRMU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SMU_465

Organism

Streptococcus mutans [Complete proteome] [HAMAP]

Taxonomic identifier

1309 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152205
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CWY4 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 43FD906A263730DC	FASTA	274	30,174
	10 20 30 40 50 60 MSLQEDIITQ LGVKPKIDAQ EEIRKSIDFL KAYMKKHGFL KSYVLGISGG QDSSLAGRLA 70 80 90 100 110 120 QLAIEELRHE TGDNGYKFIA IRLPYGVQAD EDDAQRALNF IQPDVSLAIN IKPAVDGEVA 130 140 150 160 170 180 ALAEAGVQVS DFNKGNIKAR QRMISQYAVA GENGGAVIGT DHAAENITGF FTKFGDGGAD 190 200 210 220 230 240 ILPLYRLNKR QGKQLLAELG ADKALYEKIP TADLEENKPG IADEVALGVT YNDIDDYLEG 250 260 270 KQVSPAAQKI IENWWNKTEH KRHLPISIFD DFWK

References

[1]

"Genome sequence of Streptococcus mutans UA159, a cariogenic dental pathogen." Ajdic D.J., McShan W.M., McLaughlin R.E., Savic G., Chang J., Carson M.B., Primeaux C., Tian R., Kenton S., Jia H.G., Lin S.P., Qian Y., Li S., Zhu H., Najar F.Z., Lai H., White J., Roe B.A., Ferretti J.J. Proc. Natl. Acad. Sci. U.S.A. 99:14434-14439(2002) [PubMed: 12397186] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700610 / UA159 / Serotype c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014133 Genomic DNA. Translation: AAN58214.1.
RefSeq	NP_720908.1. NC_004350.2.
3D structure databases
ProteinModelPortal	Q8CWY4.
SMR	Q8CWY4. Positions 2-274.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000014273; EBSTRP00000013755; EBSTRG00000014273.
GeneID	1027968.
GenomeReviews	Gene locus SMU_465 in contig AE014133_GR.
KEGG	smu:SMU_465.
NMPDR	fig|210007.1.peg.421.
PATRIC	19663151. VBIStrMut61772_0408.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027750.
HOGENOM	HBG351567.
OMA	IAQYEIA.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	SMUT210007:SMU_465-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STRMU

Accession

Primary (citable) accession number: Q8CWY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program