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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

metE

Organism
Streptococcus mutans serotype c (strain ATCC 700610 / UA159)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.UniRule annotation

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi630ZincUniRule annotation1
Metal bindingi632ZincUniRule annotation1
Metal bindingi715ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.1.1.14. 5941.
UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseUniRule annotation (EC:2.1.1.14UniRule annotation)
Alternative name(s):
Cobalamin-independent methionine synthaseUniRule annotation
Methionine synthase, vitamin-B12 independent isozymeUniRule annotation
Gene namesi
Name:metEUniRule annotation
Ordered Locus Names:SMU_873
OrganismiStreptococcus mutans serotype c (strain ATCC 700610 / UA159)
Taxonomic identifieri210007 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000002512 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000986701 – 7455-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST745

Interactioni

Protein-protein interaction databases

STRINGi210007.SMU_873.

Structurei

Secondary structure

1745
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi17 – 26Combined sources10
Helixi32 – 52Combined sources21
Beta strandi56 – 61Combined sources6
Helixi68 – 75Combined sources8
Helixi81 – 83Combined sources3
Helixi90 – 98Combined sources9
Beta strandi101 – 103Combined sources3
Beta strandi108 – 110Combined sources3
Beta strandi117 – 119Combined sources3
Helixi134 – 146Combined sources13
Helixi147 – 149Combined sources3
Beta strandi150 – 155Combined sources6
Helixi157 – 162Combined sources6
Helixi170 – 190Combined sources21
Beta strandi195 – 199Combined sources5
Helixi201 – 204Combined sources4
Helixi207 – 211Combined sources5
Helixi212 – 225Combined sources14
Beta strandi230 – 234Combined sources5
Helixi243 – 246Combined sources4
Beta strandi252 – 261Combined sources10
Helixi262 – 270Combined sources9
Turni271 – 276Combined sources6
Beta strandi277 – 284Combined sources8
Beta strandi286 – 288Combined sources3
Helixi294 – 306Combined sources13
Beta strandi308 – 317Combined sources10
Helixi319 – 321Combined sources3
Helixi334 – 337Combined sources4
Helixi343 – 357Combined sources15
Helixi364 – 376Combined sources13
Helixi379 – 381Combined sources3
Helixi388 – 390Combined sources3
Helixi399 – 410Combined sources12
Turni430 – 432Combined sources3
Helixi444 – 464Combined sources21
Beta strandi467 – 469Combined sources3
Helixi481 – 484Combined sources4
Beta strandi487 – 491Combined sources5
Beta strandi498 – 501Combined sources4
Beta strandi504 – 506Combined sources3
Beta strandi510 – 517Combined sources8
Helixi523 – 531Combined sources9
Beta strandi537 – 542Combined sources6
Helixi544 – 550Combined sources7
Beta strandi555 – 557Combined sources3
Helixi559 – 579Combined sources21
Beta strandi584 – 589Combined sources6
Helixi592 – 595Combined sources4
Helixi600 – 618Combined sources19
Beta strandi619 – 621Combined sources3
Beta strandi625 – 631Combined sources7
Turni637 – 639Combined sources3
Helixi640 – 646Combined sources7
Beta strandi649 – 653Combined sources5
Helixi656 – 658Combined sources3
Helixi661 – 663Combined sources3
Helixi664 – 668Combined sources5
Beta strandi674 – 678Combined sources5
Helixi690 – 698Combined sources9
Helixi699 – 702Combined sources4
Helixi706 – 708Combined sources3
Beta strandi709 – 712Combined sources4
Beta strandi717 – 720Combined sources4
Helixi722 – 741Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NQ5X-ray1.90A2-745[»]
3L7RX-ray2.40A1-745[»]
3T0CX-ray2.19A1-745[»]
ProteinModelPortaliQ8CWX6.
SMRiQ8CWX6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8CWX6.

Family & Domainsi

Sequence similaritiesi

Belongs to the vitamin-B12 independent methionine synthase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4107QYE. Bacteria.
COG0620. LUCA.
KOiK00549.
OMAiGRNIWKN.
PhylomeDBiQ8CWX6.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiView protein in InterPro
IPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
PfamiView protein in Pfam
PF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8CWX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKVSSLGYP RLGENREWKK LIEAYWAGKV SKNDLFAGAK ELRLDFLKKQ
60 70 80 90 100
LNAGLDLIPV GDFSLYDHIL DLSVQFNIIP KRFAKEPIDI DLYFAIARGN
110 120 130 140 150
KENVASSMKK WFNTNYHYIV PEWSKQRPKL NNNRLLDLYL EAREVVGDKA
160 170 180 190 200
KPVITGPITY VALSTGVEDF TAAVKSLLPL YKQVFTELVK AGASYIQVDE
210 220 230 240 250
PIFVTDEGKD YLQAAKAVYA YFAKEVPDAK FIFQTYFEGL IDSQVLSQLP
260 270 280 290 300
VDAFGLDFVY GLEENLEAIK TGAFKGKEIF AGVIDGRNIW SSDFVKTSAL
310 320 330 340 350
LETIEEQSAA LTIQPSCSLL HVPVTTKNET DLDPVLRNGL AFADEKLTEV
360 370 380 390 400
KRLAEHLDGR EDPAYDLHIA HFDALQAADF RNVKLEDLSR VATKRPSDFA
410 420 430 440 450
KRRDIQQEKL HLPLLPTTTI GSFPQSREIR RTRLAWKRGD ISDAEYKQFI
460 470 480 490 500
QAEIERWIRI QEDLDLDVLV HGEFERVDMV EFFGQKLAGF TTTKFGWVQS
510 520 530 540 550
YGSRAVKPPI IYGDVQHLEP ITVEETVYAQ SLTDRPVKGM LTGPITITNW
560 570 580 590 600
SFERTDIPRD QLFNQIGLAI KDEIKLLENA GIAIIQVDEA ALREGLPLRK
610 620 630 640 650
SKQKAYLDDA VHAFHIATSS VKDETQIHTH MCYSKFDEII DAIRALDADV
660 670 680 690 700
ISIETSRSHG DIIESFETAV YPLGIGLGVY DIHSPRVPTK EEVVANIERP
710 720 730 740
LRQLSPTQFW VNPDCGLKTR QEPETIAALK VLVAATKEVR QKLGN
Length:745
Mass (Da):84,141
Last modified:March 1, 2003 - v1
Checksum:i9CD5B8905192D823
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014133 Genomic DNA. Translation: AAN58588.1.
RefSeqiNP_721282.1. NC_004350.2.
WP_002262021.1. NC_004350.2.

Genome annotation databases

EnsemblBacteriaiAAN58588; AAN58588; SMU_873.
GeneIDi1028236.
KEGGismu:SMU_873.
PATRICifig|210007.7.peg.779.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMETE_STRMU
AccessioniPrimary (citable) accession number: Q8CWX6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: March 1, 2003
Last modified: June 7, 2017
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families