Reviewed, UniProtKB/Swiss-Prot Q8CWK6 (CYSH_VIBVU)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	VV1_1404

Organism

Vibrio vulnificus [Complete proteome] [HAMAP]

Taxonomic identifier

672 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Vibrionales › Vibrionaceae › Vibrio

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100654

Sequences

	Sequence		Length	Mass (Da)
	Q8CWK6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: C5078F647D681687	FASTA	258	29,448
	10 20 30 40 50 60 MLDSVASTLQ LSELLSLTKA EQSIRLAEIN VELEMLSAQE RVAWALQNLE GAHAVSSSFG 70 80 90 100 110 120 IQAAVMLHLV SKQQADIPVI LTDTGYLFPE TYQFIDELTK SLNLNLKVYR ANESANWQEA 130 140 150 160 170 180 RYGKLWEQGI EGIEKYNKLN KVEPMRRALN ELNVKTWFSG LRREQSQSRA GLPILSIQNG 190 200 210 220 230 240 VFKFLPVVDW SNKDVHYYLK EHGLSYHPLW EQGYLSVGDT HTTQKWEPGM SEEETRFFGL 250 KRECGLHEED NEQDGSGI

References

[1]	"Complete genome sequence of Vibrio vulnificus CMCP6." Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CMCP6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016795 Genomic DNA. Translation: AAO09853.1.
RefSeq	NP_760326.1.
3D structure databases
HSSP	HSSP built from PDB template 1SUR based on UniProtKB P17854.
SMR	Q8CWK6. Positions 7-235.
ModBase	Search...
Genome annotation databases
GeneID	1178325.
GenomeReviews	Gene locus VV1_1404 in contig AE016795_GR.
KEGG	vvu:VV1_1404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CWK6.
OMA	Q8CWK6. TRFNGLK.
Enzyme and pathway databases
BioCyc	VVUL216895:VV1_1404-MON.
BRENDA	1.8.4.8. 277169.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_VIBVU

Accession

Primary (citable) accession number: Q8CWK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)