Reviewed, UniProtKB/Swiss-Prot Q8CVZ3 (TORZ_ECOL6)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase 2
      Short name=TMAO reductase 2
      Short name=Trimethylamine oxidase 2
    EC=1.7.2.3

Gene names

Name:	torZ
Synonyms:	bisZ
Ordered Locus Names:	c2286

Organism

Escherichia coli O6 [Complete proteome] [HAMAP]

Taxonomic identifier

217992 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	809 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions. Can also reduce other N- and S-oxide compounds such as 4-methylmorpholine-N-oxide and biotin sulfoxide (BSO), but with a lower catalytic efficiency By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Miscellaneous	Expression of torYZ allows E.coli to grow anaerobically on a wider range of substrates than does expression of torCAD By similarity.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Tat-type signal Potential
	Chain	32 – 809	778	Trimethylamine-N-oxide reductase 2		PRO_0000019164

Sequences

	Sequence		Length	Mass (Da)
	Q8CVZ3-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 07087EB335CDEF22	FASTA	809	89,006
	10 20 30 40 50 60 MTLTRREFIK HSGIAAGTLV VTSAAPLPAW AEEKGGKILT AGRWGAMNVE VKDGKIVSST 70 80 90 100 110 120 GALAKTIPNS LQSTAADQVH TTARIQHPMV RKSYLDNPLQ PVKGRGEDTY VQVSWEQALK 130 140 150 160 170 180 LIHEQHDRIR KANGPSAIFA GSYGWRSSGV LHKAQTLLQR YMNLAGGYSG HSGDYSTGAA 190 200 210 220 230 240 QVIMPHVVGS VEVYEQQTSW PLILENSQVV VLWGMNPLNT LKIAWSSTDE QGLEYFHQLK 250 260 270 280 290 300 KSGKPVIAID PIRSETIEFF GDNATWIAPN MGTDVALMLG IAHTLMTQGK HDKVFLEKYT 310 320 330 340 350 360 TGYPQFEEYL TGKSDNTPKS AAWAAEITGV PEAQIVKLAE LMAANRTMLM AGWGIQRQQY 370 380 390 400 410 420 GEQKHWMLVT LAAMLGQIGT PGGGFGFSYH YSNGGNPTRV GGVLPEMSAA IAGQASEAAD 430 440 450 460 470 480 DGGMTAIPVA RIVDALENPG GKYQHNGKEQ TYPNIKMIWW AGGGNFTHHQ DTNRLIKAWQ 490 500 510 520 530 540 KPEMIVVSEC YWTAAAKHAD IVLPITTSFE RNDLTMTGDY SNQHIVLMKQ AVAPQFEARN 550 560 570 580 590 600 DFDVFADLAE LLKPGGKEIY TEGKDEMAWL KFFYDAAQKG ARAQRVTMPM FNAFWQQNKL 610 620 630 640 650 660 IEMRRSEKNE QYIRYGDFRA DPVKNALGTP SGKIEIYSRT LEKFGYKDCP AHPTWLAPDE 670 680 690 700 710 720 WKGTADEKQL QLLTAHPAHR LHSQLNYAEL RKKYAVADRE PITIHTEDAA RFGIANGDLV 730 740 750 760 770 780 RVWNKRGQIL TGAVVTDGIK KGVVCVHEGA WPDLENGLCK NGSANVLTAD IPSSQLANAC 790 800 AGNSALVYIE KYTGNALKLT AFDQPAVQA

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed: 12471157] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O6:H1 / CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014075 Genomic DNA. Translation: AAN80743.1. Different initiation.
RefSeq	NP_754178.1.
3D structure databases
HSSP	HSSP built from PDB template 1E61 based on UniProtKB Q52675.
ModBase	Search...
Genome annotation databases
GeneID	1036854.
GenomeReviews	Gene locus c2286 in contig AE014075_GR.
KEGG	ecc:c2286.
NMPDR	fig|199310.1.peg.2221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CVZ3.
OMA	Q8CVZ3. MIREEVM.
Enzyme and pathway databases
BRENDA	1.7.2.3. 292881.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006658. BisC. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006657. MPT_dinuc_bd. IPR006311. Tat. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR00509. bisC_fam. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORZ_ECOL6

Accession

Primary (citable) accession number: Q8CVZ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 45 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)