Q8CVS2 (PTRA_ECOL6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protease 3
EC=3.4.24.55

Alternative name(s):
Pitrilysin
Protease III
Protease pi

Gene names

Name:	ptrA
Synonyms:	ptr
Ordered Locus Names:	c3415

Organism

Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC) [Complete proteome] [HAMAP]

Taxonomic identifier

199310 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	962 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endopeptidase that degrades small peptides of less than 7 kDa, such as glucagon and insulin By similarity.
Catalytic activity	Preferential cleavage of 16-Tyr-|-Leu-17 and 25-Phe-|-Tyr-26 bonds of oxidized insulin B chain. Also acts on other substrates of Mw less than 7 kDa such as insulin and glucagon.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Magnesium Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 962	939	Protease 3		PRO_0000026760
Sites
	Active site	91	1	Proton acceptor By similarity
	Metal binding	88	1	Zinc By similarity
	Metal binding	92	1	Zinc By similarity
	Metal binding	169	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CVS2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: DEDD2CA2A9AADF8D	FASTA	962	107,892
	10 20 30 40 50 60 MPRSIWFKAL LLFVALWAPL SQAETGWQPI QETIRKSDKD NRQYQAIRLD NGMVVLLVSD 70 80 90 100 110 120 PQAVKSLSAL VVPVGSLEDP EAYQGLAHYL EHMSLMGSKK YPQADSLAEY LKMHGGSHNA 130 140 150 160 170 180 STAPYRTAFY LEVENDALPG AVDRLADAIA EPLLDKKYAE RERNAVNAEL TMARTRDGMR 190 200 210 220 230 240 MAQVSAETIN PAHPGSKFSG GNLETLSDKP GNPVQQALKD FHEKYYSANL MKAVIYSNKP 250 260 270 280 290 300 LPELAKMAAD TFGRVPNKES KKPEITVPVV TDAQKGIIIH YVPALPRKVL RVEFRIDNNS 310 320 330 340 350 360 AKFRSKTDEL ITYLIGNRSP GTLSDWLQKQ GLVEGISANS DPIVNGNSGV LAISASLTDK 370 380 390 400 410 420 GLANRDQVVA AIFSYLNLLR EKGIDKQYFD ELANVLDIDF RYPSITRDMD YVEWLADTMI 430 440 450 460 470 480 RVPVEHTLDA VNIADRYDAK AVKERLAMMT PQNARIWYIS PKEPHNKTAY FVDAPYQVDK 490 500 510 520 530 540 ISEQTFADWQ KKAANIALSL PELNPYIPDD FSLIKSEKKY DHPELIVDES NLRVVYAPSR 550 560 570 580 590 600 YFASEPKADV SLILRNPKAM DSARNQVMFA LNDYLAGLAL DQLSNQASVG GISFSTNANN 610 620 630 640 650 660 GLMVNANGYT QRLPQLFQAL LEGYFSYTAT EDQLEQAKSW YNQMMDSAEK GKAFEQAIMP 670 680 690 700 710 720 AQMLSQVPYF SRDERRKILP SITLKEVLAY RDALKSGARP EFMVIGNMTE AQATTLARHV 730 740 750 760 770 780 QKQLGADGSE WCRNKDVVVD KKQSVIFEKA GNSTDSALAA IFVPTGYDEY TSSAYSSLLG 790 800 810 820 830 840 QIVQPWFYNQ LRTEEQLGYA VFAFPMSVGR QWGMGFLLQS NDKQPSFLWE RYKAFFPTAE 850 860 870 880 890 900 AKLRTMKPEE FAQIQQAVIT QMLQAPQTLG EEASKLSKDF DRGNMRFDSR DKIVAQIKLL 910 920 930 940 950 960 TPQKLADFFH QAVVEPQGMA ILSQISGSQN GKAEYVHPEG WKVWENVSAL QQTMPLMSEK NE

References

[1]

"Extensive mosaic structure revealed by the complete genome sequence of uropathogenic Escherichia coli." Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D., Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F., Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T., Donnenberg M.S., Blattner F.R. Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CFT073 / ATCC 700928 / UPEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014075 Genomic DNA. Translation: AAN81860.1.
RefSeq	NP_755290.1. NC_004431.1.
3D structure databases
ProteinModelPortal	Q8CVS2.
SMR	Q8CVS2. Positions 24-960.
ModBase	Search...
Protein-protein interaction databases
STRING	199310.c3415.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN81860; AAN81860; c3415.
GeneID	1039287.
KEGG	ecc:c3415.
PATRIC	18284658. VBIEscCol75197_3215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000124324.
KO	K01407.
OMA	QAKSWYA.
ProtClustDB	PRK15101.
Family and domain databases
Gene3D	3.30.830.10. 4 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 2 hits. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 4 hits.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTRA_ECOL6

Accession

Primary (citable) accession number: Q8CVS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program