Q8CUH5 (FUMC_OCEIH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fumarate hydratase class II
Short name=Fumarase C
EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	OB1132

Organism

Oceanobacillus iheyensis (strain DSM 14371 / JCM 11309 / KCTC 3954 / HTE831) [Complete proteome] [HAMAP]

Taxonomic identifier

221109 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Oceanobacillus

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161293
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	99	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CUH5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 44242B3859AA4CDF	FASTA	461	50,732
	10 20 30 40 50 60 MDYRVEKDTI GEIQVPADKY WGAQTQRSKQ NFPIGNEKMP VEIIKAFAIL KRSTAEANFE 70 80 90 100 110 120 LGLMERDKME AIQYAADQVL NDSLTDHFPL VVWQTGSGTQ SNMNVNEVLA FVGNKWLQEQ 130 140 150 160 170 180 GSDLKLHPND DVNKSQSSND TYPTAMHIAA VLKLEDTVLP ALSQLKNTFA EKQSAFENIV 190 200 210 220 230 240 KIGRTHLQDA TPLTLGQEIS GWHRMLEKSE TMISESLEHL RELAIGGTAV GTGLNAHPDF 250 260 270 280 290 300 SEKVCKAIST FTNKKFISAK NKFHSLTSHD ETVYAHGALK GLAADLMKIA NDVRWLASGP 310 320 330 340 350 360 RCGIGEITIP ANEPGSSIMP GKVNPTQSEA VTMVVTQVMG NDAAIGFAAS QGNFELNVFK 370 380 390 400 410 420 PVIAYNFLQS SQLLADSIIS FDERCAVGIE PNHEQIEKNL NDSLMLVTAL NPHIGYENAA 430 440 450 460 KIAKKAFADN STLKETAVEL GLLTEEQFDE YVNPEEMTYP K

References

[1]	"Genome sequence of Oceanobacillus iheyensis isolated from the Iheya Ridge and its unexpected adaptive capabilities to extreme environments." Takami H., Takaki Y., Uchiyama I. Nucleic Acids Res. 30:3927-3935(2002) [PubMed: 12235376] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14371 / JCM 11309 / KCTC 3954 / HTE831.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000028 Genomic DNA. Translation: BAC13088.1.
RefSeq	NP_692053.1. NC_004193.1.
3D structure databases
ProteinModelPortal	Q8CUH5.
SMR	Q8CUH5. Positions 4-457.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1017212.
GenomeReviews	Gene locus OB1132 in contig BA000028_GR.
KEGG	oih:OB1132.
NMPDR	fig|221109.1.peg.1133.
PATRIC	22793268. VBIOceIhe82024_1151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284369.
OMA	RIEKDTM.
PhylomeDB	Q8CUH5.
ProtClustDB	PRK00485.
Enzyme and pathway databases
BioCyc	OIHE221109:OB1132-MONOMER.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR022761. Lyase1_N. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit.
KO	K01679.
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00149. FUMRATELYASE.
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR00979. FumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_OCEIH

Accession

Primary (citable) accession number: Q8CUH5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program