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Protein

Sensor protein kinase WalK

Gene

walK

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system WalK/WalR that regulates genes involved in autolysis and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to WalR.By similarity1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.By similarity

Enzyme regulationi

ATPase activity is inhibited by derivatives of thiazolidinone, benzamide and furan in vitro.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein kinase WalKCurated (EC:2.7.13.3By similarity)
Gene namesi
Name:walK
Synonyms:yycG
Ordered Locus Names:SE_0019
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000001411 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003530651 – 610Sensor protein kinase WalKAdd BLAST610

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei387Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi176280.SE0019.

Structurei

3D structure databases

ProteinModelPortaliQ8CU87.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini206 – 258HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini263 – 334PASPROSITE-ProRule annotationAdd BLAST72
Domaini327 – 380PACPROSITE-ProRule annotationAdd BLAST54
Domaini384 – 602Histidine kinasePROSITE-ProRule annotationAdd BLAST219

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK07652.
OMAiSDGAIND.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CU87-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLKQLQSL HTKLVIVYVL LIIIGMQIIG LYFTNSLEKE LLDNFKKNIT
60 70 80 90 100
QYAKQLDVNI EKVYKDKDKG SVNAQKDIQD LLNEYANRQE IGEIRFIDKD
110 120 130 140 150
QIIMATTKQS NRGLINQKVN DGSVQKALSL GQTNDHMVLK DYGSGKERVW
160 170 180 190 200
VYNIPVKVDK QTIGDIYIES KINDVYNQLN NINQIFIVGT AISLFITVIL
210 220 230 240 250
GFFIARTITK PITDMRNQTV EMSKGNYTQR VKIYGNDEIG ELALAFNNLS
260 270 280 290 300
KRVQEAQANT ESEKRRLDSV ITHMSDGILA TDRRGRVRIA NDMALKMLGL
310 320 330 340 350
AKEDVIGYYM LGVLNLENEF SLEEIQENSD SFLLDINEEE GIIARVNFST
360 370 380 390 400
IVQETGFVTG YIAVLHDVTE QQQVERERRE FVANVSHELR TPLTSMNSYI
410 420 430 440 450
EALEEGAWQD KELAPSFLSV TREETERMIR LVNDLLQLSK MDNESDQITK
460 470 480 490 500
EIIDFNMFIN KIINRHEMAA KDTTFVREIP QQTIFAEIDP DKMTQVFDNV
510 520 530 540 550
ITNAMKYSRG EKRVEFHVKQ NALYNRMTIR IKDNGIGIPI NKVDKIFDRF
560 570 580 590 600
YRVDKARTRK MGGTGLGLAI SKEIVEAHNG RIWANSVEGQ GTSIFITLPC
610
EIIEDGDWDE
Length:610
Mass (Da):69,944
Last modified:March 1, 2003 - v1
Checksum:i1ECE9A660C6EF6D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY864800 Genomic DNA. Translation: AAW62232.1.
AE015929 Genomic DNA. Translation: AAO03616.1.
RefSeqiNP_763574.1. NC_004461.1.
WP_002437327.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO03616; AAO03616; SE_0019.
GeneIDi1056847.
KEGGisep:SE0019.
PATRICi19605881. VBIStaEpi113981_0019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY864800 Genomic DNA. Translation: AAW62232.1.
AE015929 Genomic DNA. Translation: AAO03616.1.
RefSeqiNP_763574.1. NC_004461.1.
WP_002437327.1. NC_004461.1.

3D structure databases

ProteinModelPortaliQ8CU87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176280.SE0019.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO03616; AAO03616; SE_0019.
GeneIDi1056847.
KEGGisep:SE0019.
PATRICi19605881. VBIStaEpi113981_0019.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
KOiK07652.
OMAiSDGAIND.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWALK_STAES
AccessioniPrimary (citable) accession number: Q8CU87
Secondary accession number(s): Q5G1P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.