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Q8CTX5

- PFLA_STAES

UniProt

Q8CTX5 - PFLA_STAES

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Protein

Pyruvate formate-lyase-activating enzyme

Gene
pflA, SE_0215
Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activityi

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-217-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
Short name:
PFL-activating enzyme
Gene namesi
Name:pflA
Ordered Locus Names:SE_0215
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001411: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Pyruvate formate-lyase-activating enzymePRO_0000271718Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176280.SE0215.

Structurei

3D structure databases

ProteinModelPortaliQ8CTX5.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1180.
KOiK04069.
OMAiGRSNANT.
OrthoDBiEOG64FKHC.

Family and domain databases

InterProiIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02493. PFLA. 1 hit.
PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CTX5-1 [UniParc]FASTAAdd to Basket

« Hide

MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE    50
VTVDEMVNEI LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV 100
HTCIDTSAGC VNDTPAFNRH FDELQKHTDL ILLDIKHIDN DKHIKLTGKP 150
NTHILKFARK LSDMKQPVWI RHVLVPGISD DKEDLIKLGE FINSLDNVEK 200
FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY VNFNGKIPVT 250
L 251
Length:251
Mass (Da):28,555
Last modified:March 1, 2003 - v1
Checksum:i90EC4BF0E80399E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO03812.1.
RefSeqiNP_763770.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO03812; AAO03812; SE_0215.
GeneIDi1056332.
KEGGisep:SE0215.
PATRICi19606231. VBIStaEpi113981_0194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO03812.1 .
RefSeqi NP_763770.1. NC_004461.1.

3D structure databases

ProteinModelPortali Q8CTX5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176280.SE0215.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO03812 ; AAO03812 ; SE_0215 .
GeneIDi 1056332.
KEGGi sep:SE0215.
PATRICi 19606231. VBIStaEpi113981_0194.

Phylogenomic databases

eggNOGi COG1180.
KOi K04069.
OMAi GRSNANT.
OrthoDBi EOG64FKHC.

Enzyme and pathway databases

BioCyci SEPI176280:GCDG-217-MONOMER.

Family and domain databases

InterProi IPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view ]
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02493. PFLA. 1 hit.
PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiPFLA_STAES
AccessioniPrimary (citable) accession number: Q8CTX5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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