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Q8CTX5 (PFLA_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate formate-lyase-activating enzyme

Short name=PFL-activating enzyme
EC=1.97.1.4
Gene names
Name:pflA
Ordered Locus Names:SE_0215
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.

Catalytic activity

S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the organic radical-activating enzymes family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Pyruvate formate-lyase-activating enzyme
PRO_0000271718

Sites

Metal binding291Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding331Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding361Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CTX5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 90EC4BF0E80399E3

FASTA25128,555
        10         20         30         40         50         60 
MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE VTVDEMVNEI 

        70         80         90        100        110        120 
LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV HTCIDTSAGC VNDTPAFNRH 

       130        140        150        160        170        180 
FDELQKHTDL ILLDIKHIDN DKHIKLTGKP NTHILKFARK LSDMKQPVWI RHVLVPGISD 

       190        200        210        220        230        240 
DKEDLIKLGE FINSLDNVEK FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY 

       250 
VNFNGKIPVT L 

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015929 Genomic DNA. Translation: AAO03812.1.
RefSeqNP_763770.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CTX5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176280.SE0215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO03812; AAO03812; SE_0215.
GeneID1056332.
KEGGsep:SE0215.
PATRIC19606231. VBIStaEpi113981_0194.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1180.
KOK04069.
OMAYVHSVES.
OrthoDBEOG64FKHC.
ProtClustDBCLSK884495.

Enzyme and pathway databases

BioCycSEPI176280:GCDG-217-MONOMER.

Family and domain databases

InterProIPR012838. PFL_activating.
IPR001989. Radical_activat_CS.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
TIGRFAMsTIGR02493. PFLA. 1 hit.
PROSITEPS01087. RADICAL_ACTIVATING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFLA_STAES
AccessionPrimary (citable) accession number: Q8CTX5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: March 1, 2003
Last modified: November 13, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families