Reviewed, UniProtKB/Swiss-Prot Q8CTX5 (PFLA_STAES)

Names and origin

Protein names

Recommended name:
    Pyruvate formate-lyase-activating enzyme
      Short name=PFL-activating enzyme
    EC=1.97.1.4

Gene names

Name:	pflA
Ordered Locus Names:	SE_0215

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine By similarity.
Catalytic activity	S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the organic radical-activating enzymes family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW oxygen and reactive oxygen species metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW [formate-C-acetyltransferase]-activating enzyme activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Pyruvate formate-lyase-activating enzyme		PRO_0000271718
Sites
	Metal binding	29	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	33	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	36	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CTX5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 90EC4BF0E80399E3	FASTA	251	28,555
	10 20 30 40 50 60 MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE VTVDEMVNEI 70 80 90 100 110 120 LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV HTCIDTSAGC VNDTPAFNRH 130 140 150 160 170 180 FDELQKHTDL ILLDIKHIDN DKHIKLTGKP NTHILKFARK LSDMKQPVWI RHVLVPGISD 190 200 210 220 230 240 DKEDLIKLGE FINSLDNVEK FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY 250 VNFNGKIPVT L

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015929 Genomic DNA. Translation: AAO03812.1.
RefSeq	NP_763770.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1056332.
GenomeReviews	Gene locus SE_0215 in contig AE015929_GR.
KEGG	sep:SE0215.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8CTX5.
OMA	Q8CTX5. RFVVFMQ.
Enzyme and pathway databases
BioCyc	SEPI176280:SE_0215-MON.
Family and domain databases
InterPro	IPR012838. PFLA. IPR001989. Radical_activat_CS. IPR007197. Radical_SAM. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
TIGRFAMs	TIGR02493. PFLA. 1 hit.
PROSITE	PS01087. RADICAL_ACTIVATING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PFLA_STAES

Accession

Primary (citable) accession number: Q8CTX5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)