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Q8CTX5

- PFLA_STAES

UniProt

Q8CTX5 - PFLA_STAES

Protein

Pyruvate formate-lyase-activating enzyme

Gene

pflA

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S-adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + dihydroflavodoxin + [formate C-acetyltransferase]-glycine = 5'-deoxyadenosine + L-methionine + flavodoxin semiquinone + [formate C-acetyltransferase]-glycin-2-yl radical.

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi29 – 291Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi33 – 331Iron-sulfur (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi36 – 361Iron-sulfur (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. [formate-C-acetyltransferase]-activating enzyme activity Source: UniProtKB-EC
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glucose metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSEPI176280:GCDG-217-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate formate-lyase-activating enzyme (EC:1.97.1.4)
    Short name:
    PFL-activating enzyme
    Gene namesi
    Name:pflA
    Ordered Locus Names:SE_0215
    OrganismiStaphylococcus epidermidis (strain ATCC 12228)
    Taxonomic identifieri176280 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000001411: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Pyruvate formate-lyase-activating enzymePRO_0000271718Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi176280.SE0215.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8CTX5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1180.
    KOiK04069.
    OMAiGRSNANT.
    OrthoDBiEOG64FKHC.

    Family and domain databases

    InterProiIPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR02493. PFLA. 1 hit.
    PROSITEiPS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8CTX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKGHLHSVE SMGTVDGPGL RYILFTQGCL LRCLYCHNPD TWKINEPSRE    50
    VTVDEMVNEI LPYKPYFEAS GGGVTVSGGE PLLQMPFLEQ LFKELKANGV 100
    HTCIDTSAGC VNDTPAFNRH FDELQKHTDL ILLDIKHIDN DKHIKLTGKP 150
    NTHILKFARK LSDMKQPVWI RHVLVPGISD DKEDLIKLGE FINSLDNVEK 200
    FEILPYHQLG VHKWKNLGIP YQLENVEPPD DEAVKEAYRY VNFNGKIPVT 250
    L 251
    Length:251
    Mass (Da):28,555
    Last modified:March 1, 2003 - v1
    Checksum:i90EC4BF0E80399E3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO03812.1.
    RefSeqiNP_763770.1. NC_004461.1.

    Genome annotation databases

    EnsemblBacteriaiAAO03812; AAO03812; SE_0215.
    GeneIDi1056332.
    KEGGisep:SE0215.
    PATRICi19606231. VBIStaEpi113981_0194.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015929 Genomic DNA. Translation: AAO03812.1 .
    RefSeqi NP_763770.1. NC_004461.1.

    3D structure databases

    ProteinModelPortali Q8CTX5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176280.SE0215.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAO03812 ; AAO03812 ; SE_0215 .
    GeneIDi 1056332.
    KEGGi sep:SE0215.
    PATRICi 19606231. VBIStaEpi113981_0194.

    Phylogenomic databases

    eggNOGi COG1180.
    KOi K04069.
    OMAi GRSNANT.
    OrthoDBi EOG64FKHC.

    Enzyme and pathway databases

    BioCyci SEPI176280:GCDG-217-MONOMER.

    Family and domain databases

    InterProi IPR012838. PFL_activating.
    IPR001989. Radical_activat_CS.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR02493. PFLA. 1 hit.
    PROSITEi PS01087. RADICAL_ACTIVATING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
      Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
      Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 12228.

    Entry informationi

    Entry nameiPFLA_STAES
    AccessioniPrimary (citable) accession number: Q8CTX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3