Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8CT69 (SYW_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:SE_0685
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136681

Regions

Motif10 – 189"HIGH" region HAMAP-Rule MF_00140
Motif193 – 1975"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site1961ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CT69 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 274C1EB9EBC89356

FASTA32936,970
        10         20         30         40         50         60 
METLFSGIQP SGIPTIGNYI GALKQFVDVQ DDYECFFCIV DQHAITVPQD RLKLRKQIRQ 

        70         80         90        100        110        120 
LAAIYLATGI DPDKSTLFIQ SEVPAHVQAG WMLTTIASIG ELERMTQFKD KAQKRADGVP 

       130        140        150        160        170        180 
AGLLTYPPLM AADIVIYNTN IVPVGDDQKQ HMELTRNLVD RFNSRYNDVL VKPEVRMPKV 

       190        200        210        220        230        240 
GGRVMSLQDP TKKMSKSDDN QKNFISLLDE PHVAAKKIKS AVTDSDGIIK FDRENKPGIS 

       250        260        270        280        290        300 
NLLSIYSGLT NESIKNIESK YEGEGYGKFK GDLSEIVKDF LINFQEKYAS FYNSDDLDDI 

       310        320 
LDKGKEKAQK ASFKTLKKME KAMGLGRKR 

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015929 Genomic DNA. Translation: AAO04282.1.
RefSeqNP_764240.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CT69.
SMRQ8CT69. Positions 1-329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176280.SE0685.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO04282; AAO04282; SE_0685.
GeneID1055790.
KEGGsep:SE0685.
PATRIC19607163. VBIStaEpi113981_0659.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycSEPI176280:GCDG-688-MONOMER.
BRENDA6.1.1.2. 5875.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_STAES
AccessionPrimary (citable) accession number: Q8CT69
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries