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Q8CT30

- PUR1_STAES

UniProt

Q8CT30 - PUR1_STAES

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Protein
Amidophosphoribosyltransferase
Gene
purF, SE_0768
Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei11 – 111For GATase activity By similarity
Metal bindingi356 – 3561Magnesium By similarity
Metal bindingi357 – 3571Magnesium By similarity

GO - Molecular functioni

  1. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-773-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPATase
Gene namesi
Name:purF
Ordered Locus Names:SE_0768
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001411: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Propeptidei1 – 1010 By similarity
PRO_0000029269
Chaini11 – 494484Amidophosphoribosyltransferase
PRO_0000029270Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi176280.SE0768.

Structurei

3D structure databases

ProteinModelPortaliQ8CT30.
SMRiQ8CT30. Positions 11-471.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 231221Glutamine amidotransferase type-2
Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0034.
KOiK00764.
OMAiSGIPYEM.
OrthoDBiEOG6KT2Q1.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8CT30-1 [UniParc]FASTAAdd to Basket

« Hide

MSNYSGLNEE CGVFGIWNHP EAAQLTYMGL HSLQHRGQEG AGIVVSNHET    50
LKGERGLGLL TEAIKDEHMS NIKGYPHAIG HVRYATSGNK GIENIQPFLY 100
HFYDMSVGIC HNGNLINAKS LRQNLEEQGA IFHSSSDTEV IMHLIRRSKA 150
PTFEEALKES LRLIKGGFTF AILTKDALYG VVDPNAIRPL VVGKMENGAY 200
ILASETCAID VLGAEFIQDI HAGEYVVITD EGIEVKTYTR QTTTAISAME 250
YIYFARPDST IAGKNVHAVR KASGKRLAQE NPAKADMVIG VPNSSLSAAS 300
GYAEEIGLPY EMGLVKNQYV ARTFIQPTQE LREQGVRVKL SAVKDIVDGK 350
DIVLVDDSIV RGTTIKRIVK MLKDSGANRI HVRIASPEFM FPSFYGIDVS 400
TTAELISASK SPEEIKNHIG ADSLAYLSVD GLIESIGLDY DAPYHGLCVE 450
SFTGDYPAGL YDYEKNYKKH LSERQKSYIA NNKHYFDSEG NLHV 494
Length:494
Mass (Da):54,431
Last modified:March 1, 2003 - v1
Checksum:i60CAA1C760B66C12
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO04365.1.
RefSeqiNP_764323.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO04365; AAO04365; SE_0768.
GeneIDi1057695.
KEGGisep:SE0768.
PATRICi19607331. VBIStaEpi113981_0740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015929 Genomic DNA. Translation: AAO04365.1 .
RefSeqi NP_764323.1. NC_004461.1.

3D structure databases

ProteinModelPortali Q8CT30.
SMRi Q8CT30. Positions 11-471.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 176280.SE0768.

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAO04365 ; AAO04365 ; SE_0768 .
GeneIDi 1057695.
KEGGi sep:SE0768.
PATRICi 19607331. VBIStaEpi113981_0740.

Phylogenomic databases

eggNOGi COG0034.
KOi K00764.
OMAi SGIPYEM.
OrthoDBi EOG6KT2Q1.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci SEPI176280:GCDG-773-MONOMER.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiPUR1_STAES
AccessioniPrimary (citable) accession number: Q8CT30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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