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Q8CT28 (PUR3_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylglycinamide formyltransferase

EC=2.1.2.2
Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART
Gene names
Name:purN
Ordered Locus Names:SE_0770
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacillalesStaphylococcus

Protein attributes

Sequence length188 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the GART family.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionphosphoribosylglycinamide formyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 188188Phosphoribosylglycinamide formyltransferase
PRO_0000074951

Regions

Region12 – 1435'-phosphoribosylglycinamide binding By similarity
Region91 – 94410-formyltetrahydrofolate binding By similarity

Sites

Active site1101Proton donor By similarity
Binding site66110-formyltetrahydrofolate By similarity
Binding site108110-formyltetrahydrofolate By similarity
Binding site146110-formyltetrahydrofolate; via amide nitrogen By similarity
Binding site17515'-phosphoribosylglycinamide By similarity
Site1461Raises pKa of active site His By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CT28 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7A0D37C071678E8A

FASTA18820,965
        10         20         30         40         50         60 
MTNIAIFASG SGSNFENIVK HIQTGQLSGI NVTALYTDNE GVPCIDRAKN LNIPIHINKP 

        70         80         90        100        110        120 
KDFSSKSLYE QHLLKLLSSE EVQWIVLAGY MRLVGQDLLQ AYEGRILNIH PSLLPKFKGL 

       130        140        150        160        170        180 
DAIGQALESG DTVTGSTVHY VDSGMDTGEI IEQQQCDIKP DDTKEQLEDR VKHLEYELYP 


RVIAKIIK 

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed: 12950922] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015929 Genomic DNA. Translation: AAO04367.1.
RefSeqNP_764325.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CT28.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ8CT28.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000040105; EBSTAP00000038738; EBSTAG00000040103.
GeneID1056737.
GenomeReviewsGene locus SE_0770 in contig AE015929_GR.
KEGGsep:SE0770.
PATRIC19607335. VBIStaEpi113981_0742.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0299.
GeneTreeEBGT00050000024715.
HOGENOMHBG649382.
OMASSGFMRI.
PhylomeDBQ8CT28.
ProtClustDBCLSK885118.

Enzyme and pathway databases

BioCycSEPI176280:SE_0770-MONOMER.

Family and domain databases

InterProIPR002376. Formyl_transf_N.
IPR004607. PurN_trans.
[Graphical view]
Gene3DG3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KOK11175.
PfamPF00551. Formyl_trans_N. 1 hit.
[Graphical view]
SUPFAMSSF53328. formyl_transf. 1 hit.
TIGRFAMsTIGR00639. PurN. 1 hit.
PROSITEPS00373. GART. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR3_STAES
AccessionPrimary (citable) accession number: Q8CT28
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2003
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families