Q8CT26 (PUR2_STAES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	SE_0772

Organism

Staphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]

Taxonomic identifier

176280 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus epidermidis

Protein attributes

Sequence length	413 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence AAO04369.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 413	413	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151484
Regions
	Domain	108 – 310	203	ATP-grasp
	Nucleotide binding	134 – 190	57	ATP By similarity
Sites
	Metal binding	280	1	Magnesium or manganese By similarity
	Metal binding	282	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8CT26 [UniParc]. Last modified July 19, 2005. Version 2. Checksum: 4EEA202347FD889A	FASTA	413	45,326
	10 20 30 40 50 60 MKVLIVGAGG REHALASKIN QSPIVDKVYA IPGNDAMVNI AEVHSEIAES DHQAILHFAQ 70 80 90 100 110 120 HNAIDWVIIG PEQPLIDGLA DLLRNANIKV FGPGQDAAQI EGSKLFAKQL MDKYRIPTAE 130 140 150 160 170 180 YKEVSSRNEA LQYVETCDLP IVIKKDGLAA GKGVIIAFTR EDALDGVKKI YQEEKGKVVF 190 200 210 220 230 240 ESYLEGEEFS LMTFVNGDYA VPFDCIAQDH KRAFDNDQGP NTGGMGAYCP VPHIDASVLE 250 260 270 280 290 300 QTNKEIAQPI AKAMAQEGHD FFGLLYIGAI LTKDGPKVIE FNARFGDPEA QVLLTRLESD 310 320 330 340 350 360 LMQLIIDLEN CQPIHFNWKD EAVVGVMLAS KGYPGSYEKG HEISGFNLDS HYYVSGLKKE 370 380 390 400 410 GQCFVNSGGR VILAIGEGPT VEKAQANAYE HARQIKSDNL FYRNDIGNKA ITK

References

[1]

"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)." Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M. Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015929 Genomic DNA. Translation: AAO04369.1. Different initiation.
RefSeq	NP_764327.1. NC_004461.1.
3D structure databases
ProteinModelPortal	Q8CT26.
ModBase	Search...
Protein-protein interaction databases
STRING	176280.SE0772.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAO04369; AAO04369; SE_0772.
GeneID	1055717.
KEGG	sep:SE0772.
PATRIC	19607339. VBIStaEpi113981_0744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0151.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK13790.
Enzyme and pathway databases
BioCyc	SEPI176280:GCDG-777-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_STAES

Accession

Primary (citable) accession number: Q8CT26

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 29, 2013
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program