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Q8CT26 (PUR2_STAES) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:SE_0772
OrganismStaphylococcus epidermidis (strain ATCC 12228) [Complete proteome] [HAMAP]
Taxonomic identifier176280 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length413 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAO04369.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 413413Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151484

Regions

Domain108 – 310203ATP-grasp
Nucleotide binding134 – 19057ATP By similarity

Sites

Metal binding2801Magnesium or manganese By similarity
Metal binding2821Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8CT26 [UniParc].

Last modified July 19, 2005. Version 2.
Checksum: 4EEA202347FD889A

FASTA41345,326
        10         20         30         40         50         60 
MKVLIVGAGG REHALASKIN QSPIVDKVYA IPGNDAMVNI AEVHSEIAES DHQAILHFAQ 

        70         80         90        100        110        120 
HNAIDWVIIG PEQPLIDGLA DLLRNANIKV FGPGQDAAQI EGSKLFAKQL MDKYRIPTAE 

       130        140        150        160        170        180 
YKEVSSRNEA LQYVETCDLP IVIKKDGLAA GKGVIIAFTR EDALDGVKKI YQEEKGKVVF 

       190        200        210        220        230        240 
ESYLEGEEFS LMTFVNGDYA VPFDCIAQDH KRAFDNDQGP NTGGMGAYCP VPHIDASVLE 

       250        260        270        280        290        300 
QTNKEIAQPI AKAMAQEGHD FFGLLYIGAI LTKDGPKVIE FNARFGDPEA QVLLTRLESD 

       310        320        330        340        350        360 
LMQLIIDLEN CQPIHFNWKD EAVVGVMLAS KGYPGSYEKG HEISGFNLDS HYYVSGLKKE 

       370        380        390        400        410 
GQCFVNSGGR VILAIGEGPT VEKAQANAYE HARQIKSDNL FYRNDIGNKA ITK 

« Hide

References

[1]"Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015929 Genomic DNA. Translation: AAO04369.1. Different initiation.
RefSeqNP_764327.1. NC_004461.1.

3D structure databases

ProteinModelPortalQ8CT26.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176280.SE0772.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAO04369; AAO04369; SE_0772.
GeneID1055717.
KEGGsep:SE0772.
PATRIC19607339. VBIStaEpi113981_0744.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK13790.

Enzyme and pathway databases

BioCycSEPI176280:GCDG-777-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_STAES
AccessionPrimary (citable) accession number: Q8CT26
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: July 19, 2005
Last modified: February 19, 2014
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways