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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Staphylococcus epidermidis (strain ATCC 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei554 – 5541Aminoacyl-adenylateUniRule annotation
Binding sitei598 – 5981ATPUniRule annotation
Metal bindingi885 – 8851ZincUniRule annotation
Metal bindingi888 – 8881ZincUniRule annotation
Metal bindingi905 – 9051ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-874-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SE_0868
OrganismiStaphylococcus epidermidis (strain ATCC 12228)
Taxonomic identifieri176280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000001411: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 916916Isoleucine--tRNA ligasePRO_0000098471Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi176280.SE0868.

Structurei

3D structure databases

ProteinModelPortaliQ8CSX1.
SMRiQ8CSX1. Positions 1-916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi595 – 5995"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8CSX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNYKDTLLMP KTDFPMRGGL PNKEPQIQEM WDNDEQYRKA LEKNKNNPSF
60 70 80 90 100
ILHDGPPYAN GNLHMGHALN KIIKDFIVRY KTMQGFYAPY VPGWDTHGLP
110 120 130 140 150
IEQALTKKGV DRKKMSVAEF REKCKEFALK QIDIQKKDFK RLGVRGDFNN
160 170 180 190 200
PYITLTPEYE AAQIRLFGEM ADKGLIYKGK KPVYWSPSSE SSLAEAEIEY
210 220 230 240 250
HDKRSASIYV AFDVKDSKGK VDSDAQFIIW TTTPWTIPSN VAITVHPELN
260 270 280 290 300
YGQYNVNGHK YIVAQALSEA VAEALGWDKD SIQLEKEFTG KELEFVEAQH
310 320 330 340 350
PFLDRVSLVI NGEHVTTDAG TGCVHTAPGH GEDDYIVGQK YDLPVISPLN
360 370 380 390 400
DKGVFTEEGG PFEGMFYDKA NKAVTDLLKE KDALLKLDFI THSYPHDWRT
410 420 430 440 450
KKPVIFRATP QWFASINKVR QDILDAIEDT NFKVDWGKTR IYNMIRDRGE
460 470 480 490 500
WVISRQRVWG VPLPVFYAEN GDIIMTKETV NHVADLFEKY GSNIWFEKEA
510 520 530 540 550
KELLPEGFSH PGSPNGEFTK ETDIMDVWFD SGSSHRGVLE TRPELSFPAD
560 570 580 590 600
LYFEGSDQYR GWFNSSITTA VATRGQAPYK FLLSHGFVMD GEGKKMSKSL
610 620 630 640 650
GNVIVPDQVV KQKGADIARL WVSSTDYLSD VRISDEILKQ TSDVYRKIRN
660 670 680 690 700
TLRFMLGNIN DFNPETDSIA ETNLLEVDRY LLNRLREFTA STINNYENFD
710 720 730 740 750
YLNIYQEVQN FINVELSNFY LDYGKDILYI EKKDSHKRRS MQTVLYQILI
760 770 780 790 800
DMTKLLAPIL VHTAEEVWSH TPHVKEESVH LSDMPKVVDV DEELLEKWNT
810 820 830 840 850
FMNLRDDVNR ALEQARNEKV IGKSLEAKVV IGNNETFNTA EFLQQFNDLQ
860 870 880 890 900
QLFIVSQVEV KDKVNDGVSY QYGDIHIKHA EGEKCERCWN YTEELGSVGE
910
LEHLCPRCQE VVKTLV
Length:916
Mass (Da):105,186
Last modified:March 1, 2003 - v1
Checksum:iC5155509A91FCEEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04465.1.
RefSeqiNP_764423.1. NC_004461.1.

Genome annotation databases

EnsemblBacteriaiAAO04465; AAO04465; SE_0868.
GeneIDi1056226.
KEGGisep:SE0868.
PATRICi19607535. VBIStaEpi113981_0841.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015929 Genomic DNA. Translation: AAO04465.1.
RefSeqiNP_764423.1. NC_004461.1.

3D structure databases

ProteinModelPortaliQ8CSX1.
SMRiQ8CSX1. Positions 1-916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi176280.SE0868.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO04465; AAO04465; SE_0868.
GeneIDi1056226.
KEGGisep:SE0868.
PATRICi19607535. VBIStaEpi113981_0841.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciSEPI176280:GCDG-874-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228)."
    Zhang Y.-Q., Ren S.-X., Li H.-L., Wang Y.-X., Fu G., Yang J., Qin Z.-Q., Miao Y.-G., Wang W.-Y., Chen R.-S., Shen Y., Chen Z., Yuan Z.-H., Zhao G.-P., Qu D., Danchin A., Wen Y.-M.
    Mol. Microbiol. 49:1577-1593(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 12228.

Entry informationi

Entry nameiSYI_STAES
AccessioniPrimary (citable) accession number: Q8CSX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.